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| Name | TE buffer (1X) pH 8.0 low EDTA |
| Category | Molecular biology |
| PH | 8 |
| EDTA | Low |
| Concentration | 1X |
| Application | DNA/RNA storage and resuspension |
| Composition | Tris-HCl, EDTA |
| Storage Condition | Room temperature |
| Stability | Stable for long-term storage |
| Sterilization | Autoclavable |
| Compatibility | Compatible with nucleic acid applications |
| Formulation | Ready-to-use liquid |
| Volume | 500mL |
| Shelf Life | 1 year |
| Manufacturer | Various |
FAQ
What is TE buffer (1X) pH 8.0 with low EDTA used for in molecular biology?
TE buffer (Tris-EDTA buffer) is commonly used for the storage and handling of nucleic acids, especially DNA. This buffer helps in maintaining the stability and integrity of DNA molecules by protecting them from degradation.
What is the purpose of using EDTA in TE buffer?
EDTA (Ethylenediaminetetraacetic acid) is added to TE buffer to chelate divalent metal ions such as magnesium, which can catalyze the degradation of DNA by nucleases. By sequestering these metal ions, EDTA helps in preventing DNA degradation and maintaining the stability of nucleic acids.
Why is pH 8.0 important in TE buffer for molecular biology applications?
The pH of TE buffer is typically set to 8.0, which is close to the physiological pH of most biological systems. This pH is optimal for the stability of DNA and RNA molecules, as well as for many enzymatic reactions commonly used in molecular biology experiments.
What is the significance of using low EDTA concentration in TE buffer?
Low EDTA concentration in TE buffer helps in minimizing the chelation of essential metal ions required for enzymatic reactions. This ensures that the buffer maintains the stability of nucleic acids while allowing for the proper functioning of enzymes in molecular biology applications.
How should TE buffer (1X) pH 8.0 with low EDTA be stored for maximum effectiveness?
TE buffer should be stored at room temperature or at 4°C to maintain its stability and effectiveness. It is important to protect the buffer from light and moisture to prevent any contamination or degradation of its components.
TE buffer (Tris-EDTA buffer) is commonly used for the storage and handling of nucleic acids, especially DNA. This buffer helps in maintaining the stability and integrity of DNA molecules by protecting them from degradation.
What is the purpose of using EDTA in TE buffer?
EDTA (Ethylenediaminetetraacetic acid) is added to TE buffer to chelate divalent metal ions such as magnesium, which can catalyze the degradation of DNA by nucleases. By sequestering these metal ions, EDTA helps in preventing DNA degradation and maintaining the stability of nucleic acids.
Why is pH 8.0 important in TE buffer for molecular biology applications?
The pH of TE buffer is typically set to 8.0, which is close to the physiological pH of most biological systems. This pH is optimal for the stability of DNA and RNA molecules, as well as for many enzymatic reactions commonly used in molecular biology experiments.
What is the significance of using low EDTA concentration in TE buffer?
Low EDTA concentration in TE buffer helps in minimizing the chelation of essential metal ions required for enzymatic reactions. This ensures that the buffer maintains the stability of nucleic acids while allowing for the proper functioning of enzymes in molecular biology applications.
How should TE buffer (1X) pH 8.0 with low EDTA be stored for maximum effectiveness?
TE buffer should be stored at room temperature or at 4°C to maintain its stability and effectiveness. It is important to protect the buffer from light and moisture to prevent any contamination or degradation of its components.
