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| Name | Tris buffer pH 7.5 (1 M) |
| Brand | Molecular Biology |
| Type | Buffer solution |
| PH | 7.5 |
| Concentration | 1 M |
| Formulation | Tris base |
| Quantity | 1 L |
| Storage Conditions | Store at room temperature |
| Shelf Life | 1 year |
| Applications | N/A |
| Compatibility | Compatible with most biological assays |
| Stability | Stable for long-term storage |
| Packaging | Bottle |
| Sterilization | Sterile filtered |
| Purity | High purity |
| Solubility | Highly soluble in water |
| Recommended Usage | Dilute to desired concentration before use |
FAQ
What is Tris buffer pH 7.5 (1 M) and why is it used in molecular biology?
Tris buffer pH 7.5 (1 M) is a solution composed of Tris(hydroxymethyl)aminomethane, also known as Tris base, at a pH of 7.5 and a concentration of 1 Molar. Tris buffer is commonly used in molecular biology as a buffering agent to maintain a stable pH in various biological and biochemical experiments. The pH of the buffer is crucial for the proper functioning of enzymes and other biological molecules, making Tris buffer pH 7.5 (1 M) a popular choice for many molecular biology applications.
How is Tris buffer pH 7.5 (1 M) prepared and stored?
Tris buffer pH 7.5 (1 M) can be prepared by dissolving the appropriate amount of Tris base in water and adjusting the pH to 7.5 using a strong acid, such as hydrochloric acid. Once prepared, the buffer should be stored at room temperature and protected from light to prevent degradation. It is important to label the container clearly to avoid confusion with other solutions in the laboratory.
What are some common applications of Tris buffer pH 7.5 (1 M) in molecular biology?
Tris buffer pH 7.5 (1 M) is commonly used in molecular biology for various applications, including electrophoresis, nucleic acid isolation and purification, protein extraction, enzymatic reactions, and cell culture. The buffer's ability to maintain a stable pH in a wide range of experimental conditions makes it a versatile and essential reagent in the laboratory.
How does Tris buffer pH 7.5 (1 M) compare to other buffering agents?
Tris buffer pH 7.5 (1 M) is preferred over other buffering agents, such as phosphate and acetate buffers, for certain applications in molecular biology due to its stability at physiological pH and its lower interference with enzymatic reactions. Additionally, Tris buffer is less prone to precipitation at lower temperatures, making it suitable for experiments conducted at refrigerated conditions.
What are some precautions to consider when working with Tris buffer pH 7.5 (1 M) in the laboratory?
When working with Tris buffer pH 7.5 (1 M) in the laboratory, it is essential to wear appropriate personal protective equipment, such as gloves and safety goggles, to prevent skin and eye irritation. The buffer should be handled with care to avoid spillage and inhalation of the dust. In case of accidental exposure, rinse the affected area with water immediately and seek medical attention if necessary.
Tris buffer pH 7.5 (1 M) is a solution composed of Tris(hydroxymethyl)aminomethane, also known as Tris base, at a pH of 7.5 and a concentration of 1 Molar. Tris buffer is commonly used in molecular biology as a buffering agent to maintain a stable pH in various biological and biochemical experiments. The pH of the buffer is crucial for the proper functioning of enzymes and other biological molecules, making Tris buffer pH 7.5 (1 M) a popular choice for many molecular biology applications.
How is Tris buffer pH 7.5 (1 M) prepared and stored?
Tris buffer pH 7.5 (1 M) can be prepared by dissolving the appropriate amount of Tris base in water and adjusting the pH to 7.5 using a strong acid, such as hydrochloric acid. Once prepared, the buffer should be stored at room temperature and protected from light to prevent degradation. It is important to label the container clearly to avoid confusion with other solutions in the laboratory.
What are some common applications of Tris buffer pH 7.5 (1 M) in molecular biology?
Tris buffer pH 7.5 (1 M) is commonly used in molecular biology for various applications, including electrophoresis, nucleic acid isolation and purification, protein extraction, enzymatic reactions, and cell culture. The buffer's ability to maintain a stable pH in a wide range of experimental conditions makes it a versatile and essential reagent in the laboratory.
How does Tris buffer pH 7.5 (1 M) compare to other buffering agents?
Tris buffer pH 7.5 (1 M) is preferred over other buffering agents, such as phosphate and acetate buffers, for certain applications in molecular biology due to its stability at physiological pH and its lower interference with enzymatic reactions. Additionally, Tris buffer is less prone to precipitation at lower temperatures, making it suitable for experiments conducted at refrigerated conditions.
What are some precautions to consider when working with Tris buffer pH 7.5 (1 M) in the laboratory?
When working with Tris buffer pH 7.5 (1 M) in the laboratory, it is essential to wear appropriate personal protective equipment, such as gloves and safety goggles, to prevent skin and eye irritation. The buffer should be handled with care to avoid spillage and inhalation of the dust. In case of accidental exposure, rinse the affected area with water immediately and seek medical attention if necessary.
