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| Name | Bradford - Solution for protein determination |
| Manufacturer | Sigma-Aldrich |
| Type | Chemical reagent |
| Application | Protein determination |
| Method | Bradford assay |
| Formulation | Ready-to-use solution |
| Storage Conditions | Store at room temperature |
| Shelf Life | 12 months |
| Packaging | Bottle |
| Volume | 500 mL |
| Sensitivity | High sensitivity |
| Specificity | High specificity |
| Concentration Range | 0.1-1.0 mg/mL |
| Detection Wavelength | 595 nm |
| Compatibility | Compatible with various protein samples |
| Safety Information | Handle with care and wear appropriate personal protective equipment |
FAQ
What is Bradford Solution?
Bradford Solution is a protein determination reagent used for quantifying the concentration of proteins in a sample. It is commonly used in laboratories for a quick and easy measurement of protein levels in various biological samples.
How does Bradford Solution work?
Bradford Solution works based on the principle of protein-dye binding. Proteins in the sample bind to the Coomassie Blue dye in the reagent, causing a shift in the absorption spectrum of the dye. This shift can be measured spectrophotometrically to determine the concentration of proteins in the sample.
What are the advantages of using Bradford Solution for protein determination?
One of the main advantages of using Bradford Solution is its sensitivity. It can detect proteins in concentrations as low as 1 μg/ml, making it suitable for a wide range of protein samples. Additionally, Bradford Solution is fast and easy to use, providing quick results without complex procedures.
How is Bradford Solution different from other protein determination methods?
Bradford Solution is based on a colorimetric assay, while other methods such as Lowry assay or BCA assay rely on different principles for protein quantification. The choice of method depends on the sample type, concentration range, and desired sensitivity. Bradford Solution is often preferred for its simplicity and speed.
Is Bradford Solution suitable for all types of protein samples?
Bradford Solution is compatible with most protein samples, including purified proteins, cell lysates, and protein mixtures. However, it may not be suitable for certain samples with interfering substances that can affect the colorimetric reaction. It is always recommended to validate the method with a standard protein sample before analyzing unknown samples.
Bradford Solution is a protein determination reagent used for quantifying the concentration of proteins in a sample. It is commonly used in laboratories for a quick and easy measurement of protein levels in various biological samples.
How does Bradford Solution work?
Bradford Solution works based on the principle of protein-dye binding. Proteins in the sample bind to the Coomassie Blue dye in the reagent, causing a shift in the absorption spectrum of the dye. This shift can be measured spectrophotometrically to determine the concentration of proteins in the sample.
What are the advantages of using Bradford Solution for protein determination?
One of the main advantages of using Bradford Solution is its sensitivity. It can detect proteins in concentrations as low as 1 μg/ml, making it suitable for a wide range of protein samples. Additionally, Bradford Solution is fast and easy to use, providing quick results without complex procedures.
How is Bradford Solution different from other protein determination methods?
Bradford Solution is based on a colorimetric assay, while other methods such as Lowry assay or BCA assay rely on different principles for protein quantification. The choice of method depends on the sample type, concentration range, and desired sensitivity. Bradford Solution is often preferred for its simplicity and speed.
Is Bradford Solution suitable for all types of protein samples?
Bradford Solution is compatible with most protein samples, including purified proteins, cell lysates, and protein mixtures. However, it may not be suitable for certain samples with interfering substances that can affect the colorimetric reaction. It is always recommended to validate the method with a standard protein sample before analyzing unknown samples.
