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| Name | Bromocresol Green |
| Grade | ACS |
| Product Type | Analysis |
| Appearance | Green solid |
| Formula | C21H14Br4O5S |
| Molar Mass | 698.98 g/mol |
| Melting Point | 220-224°C |
| Solubility | Soluble in water and alcohol |
| Storage Condition | Store at room temperature in a dry and well-ventilated area |
| Shelf Life | At least 3 years when stored properly |
| Applications | Used as a pH indicator in analytical chemistry |
| Purity | ≥98% |
| Cas Number | 76-60-8 |
FAQ
What is Bromocresol Green and how is it used for analysis?
Bromocresol Green is a pH indicator dye that is commonly used in laboratory analysis. It changes color in response to changes in pH, making it a valuable tool for determining the acidity or alkalinity of a solution. In addition to its use as a pH indicator, Bromocresol Green is also used in protein electrophoresis and as a tracking dye in DNA agarose gel electrophoresis.
What are the advantages of using Bromocresol Green for analysis?
One of the main advantages of using Bromocresol Green is its high sensitivity to pH changes. It has a color transition range of 3.8-5.4, which makes it ideal for accurately determining the pH of a solution. Additionally, Bromocresol Green is stable and relatively easy to work with, making it a popular choice for a wide range of laboratory applications.
How is Bromocresol Green typically used in protein electrophoresis?
In protein electrophoresis, Bromocresol Green is often added to the sample loading buffer to track the progress of the electrophoresis process. By monitoring the movement of the Bromocresol Green dye through the gel, researchers can track the migration of proteins and ensure that the electrophoresis is proceeding as expected. Bromocresol Green is also used as a visual indicator of the pH gradient in isoelectric focusing gels, helping researchers to identify and analyze proteins based on their isoelectric point.
What are some common uses of Bromocresol Green in DNA agarose gel electrophoresis?
In DNA agarose gel electrophoresis, Bromocresol Green is often added to the DNA loading buffer to track the migration of DNA fragments through the gel. The dye migrates with the DNA bands during electrophoresis, allowing researchers to monitor the progress of the separation process. Bromocresol Green helps to visualize the DNA bands and determine the size of the fragments based on their relative migration distance.
Are there any safety considerations to keep in mind when working with Bromocresol Green?
While Bromocresol Green is generally considered safe to work with, it is important to take proper precautions when handling the dye. It is recommended to wear gloves and eye protection when using Bromocresol Green to avoid skin contact or accidental ingestion. Additionally, care should be taken to avoid inhaling the dye or exposing it to open flames, as it may be harmful if inhaled or combustible. Proper disposal procedures should also be followed to minimize environmental impact.
Bromocresol Green is a pH indicator dye that is commonly used in laboratory analysis. It changes color in response to changes in pH, making it a valuable tool for determining the acidity or alkalinity of a solution. In addition to its use as a pH indicator, Bromocresol Green is also used in protein electrophoresis and as a tracking dye in DNA agarose gel electrophoresis.
What are the advantages of using Bromocresol Green for analysis?
One of the main advantages of using Bromocresol Green is its high sensitivity to pH changes. It has a color transition range of 3.8-5.4, which makes it ideal for accurately determining the pH of a solution. Additionally, Bromocresol Green is stable and relatively easy to work with, making it a popular choice for a wide range of laboratory applications.
How is Bromocresol Green typically used in protein electrophoresis?
In protein electrophoresis, Bromocresol Green is often added to the sample loading buffer to track the progress of the electrophoresis process. By monitoring the movement of the Bromocresol Green dye through the gel, researchers can track the migration of proteins and ensure that the electrophoresis is proceeding as expected. Bromocresol Green is also used as a visual indicator of the pH gradient in isoelectric focusing gels, helping researchers to identify and analyze proteins based on their isoelectric point.
What are some common uses of Bromocresol Green in DNA agarose gel electrophoresis?
In DNA agarose gel electrophoresis, Bromocresol Green is often added to the DNA loading buffer to track the migration of DNA fragments through the gel. The dye migrates with the DNA bands during electrophoresis, allowing researchers to monitor the progress of the separation process. Bromocresol Green helps to visualize the DNA bands and determine the size of the fragments based on their relative migration distance.
Are there any safety considerations to keep in mind when working with Bromocresol Green?
While Bromocresol Green is generally considered safe to work with, it is important to take proper precautions when handling the dye. It is recommended to wear gloves and eye protection when using Bromocresol Green to avoid skin contact or accidental ingestion. Additionally, care should be taken to avoid inhaling the dye or exposing it to open flames, as it may be harmful if inhaled or combustible. Proper disposal procedures should also be followed to minimize environmental impact.
