Coomassie® Brilliant Blue R-250 (C.I. 42660)

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Name Coomassie® Brilliant Blue R-250
Product Code C.I. 42660
Color Blue
Form Powder
Solubility Soluble in water
Usage Staining protein gels
Storage Conditions Store at room temperature
Purity Greater than 97%
Molecular Weight 854.01 g/mol
Chemical Formula C45H44N3O7S2
Manufacturing Method Synthetic
Appearance Dark blue solid
Application Protein staining
Shelf Life 2 years
Melting Point 290-292°C
Boiling Point Not applicable
FAQ
What is Coomassie® Brilliant Blue R-250 (C.I. 42660)?
Coomassie® Brilliant Blue R-250 (C.I. 42660) is a widely used stain in protein electrophoresis and western blotting techniques. It is a dye that binds to proteins, allowing for their visualization and quantification.

How is Coomassie® Brilliant Blue R-250 used in protein electrophoresis?
Coomassie® Brilliant Blue R-250 is added to the protein samples prior to running them on a gel. After electrophoresis, the gel is soaked in a solution containing the dye, which binds to the proteins. The stained proteins can then be visualized and analyzed.

What are the advantages of using Coomassie® Brilliant Blue R-250 in protein electrophoresis?
Coomassie® Brilliant Blue R-250 is known for its sensitivity and compatibility with a wide range of protein samples. It is a cost-effective option for protein staining and provides clear, sharp bands for easy interpretation of results.

Can Coomassie® Brilliant Blue R-250 be used in western blotting?
Yes, Coomassie® Brilliant Blue R-250 can be used in western blotting as a protein stain. After transferring proteins from a gel to a membrane, the membrane can be stained with the dye to visualize the proteins of interest.

Is Coomassie® Brilliant Blue R-250 suitable for my research needs?
Coomassie® Brilliant Blue R-250 is a versatile protein stain that is suitable for a wide range of research applications. Whether you are performing routine protein electrophoresis or advanced western blotting experiments, Coomassie® Brilliant Blue R-250 can help you achieve accurate and reliable results.
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