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Name | Coomassie® Brilliant Blue R-250 |
Product Code | C.I. 42660 |
Color | Blue |
Form | Powder |
Solubility | Soluble in water |
Usage | Staining protein gels |
Storage Conditions | Store at room temperature |
Purity | Greater than 97% |
Molecular Weight | 854.01 g/mol |
Chemical Formula | C45H44N3O7S2 |
Manufacturing Method | Synthetic |
Appearance | Dark blue solid |
Application | Protein staining |
Shelf Life | 2 years |
Melting Point | 290-292°C |
Boiling Point | Not applicable |
FAQ
What is Coomassie® Brilliant Blue R-250 (C.I. 42660)?
Coomassie® Brilliant Blue R-250 (C.I. 42660) is a widely used stain in protein electrophoresis and western blotting techniques. It is a dye that binds to proteins, allowing for their visualization and quantification.
How is Coomassie® Brilliant Blue R-250 used in protein electrophoresis?
Coomassie® Brilliant Blue R-250 is added to the protein samples prior to running them on a gel. After electrophoresis, the gel is soaked in a solution containing the dye, which binds to the proteins. The stained proteins can then be visualized and analyzed.
What are the advantages of using Coomassie® Brilliant Blue R-250 in protein electrophoresis?
Coomassie® Brilliant Blue R-250 is known for its sensitivity and compatibility with a wide range of protein samples. It is a cost-effective option for protein staining and provides clear, sharp bands for easy interpretation of results.
Can Coomassie® Brilliant Blue R-250 be used in western blotting?
Yes, Coomassie® Brilliant Blue R-250 can be used in western blotting as a protein stain. After transferring proteins from a gel to a membrane, the membrane can be stained with the dye to visualize the proteins of interest.
Is Coomassie® Brilliant Blue R-250 suitable for my research needs?
Coomassie® Brilliant Blue R-250 is a versatile protein stain that is suitable for a wide range of research applications. Whether you are performing routine protein electrophoresis or advanced western blotting experiments, Coomassie® Brilliant Blue R-250 can help you achieve accurate and reliable results.
Coomassie® Brilliant Blue R-250 (C.I. 42660) is a widely used stain in protein electrophoresis and western blotting techniques. It is a dye that binds to proteins, allowing for their visualization and quantification.
How is Coomassie® Brilliant Blue R-250 used in protein electrophoresis?
Coomassie® Brilliant Blue R-250 is added to the protein samples prior to running them on a gel. After electrophoresis, the gel is soaked in a solution containing the dye, which binds to the proteins. The stained proteins can then be visualized and analyzed.
What are the advantages of using Coomassie® Brilliant Blue R-250 in protein electrophoresis?
Coomassie® Brilliant Blue R-250 is known for its sensitivity and compatibility with a wide range of protein samples. It is a cost-effective option for protein staining and provides clear, sharp bands for easy interpretation of results.
Can Coomassie® Brilliant Blue R-250 be used in western blotting?
Yes, Coomassie® Brilliant Blue R-250 can be used in western blotting as a protein stain. After transferring proteins from a gel to a membrane, the membrane can be stained with the dye to visualize the proteins of interest.
Is Coomassie® Brilliant Blue R-250 suitable for my research needs?
Coomassie® Brilliant Blue R-250 is a versatile protein stain that is suitable for a wide range of research applications. Whether you are performing routine protein electrophoresis or advanced western blotting experiments, Coomassie® Brilliant Blue R-250 can help you achieve accurate and reliable results.