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| Name | Fluorescein Sodium (C.I. 45350) |
| Cas Number | 518-47-8 |
| Molecular Formula | C20H10Na2O5 |
| Molecular Weight | 376.27 g/mol |
| Appearance | Dark orange to red powder |
| Solubility | Soluble in water |
| Melting Point | 316.5 °C |
| Boiling Point | 705.3 °C |
| Purity | ≥98% |
| Storage Conditions | Store in a cool, dry place |
| Usage | Fluorescent tracer |
| Applications | Medical diagnostics, Biochemical research |
FAQ
What is Fluorescein Sodium (C.I. 45350) and how is it used for analysis?
Fluorescein Sodium (C.I. 45350) is a synthetic organic compound commonly used in various analytical techniques such as fluorescence microscopy, flow cytometry, and spectrophotometry. It is highly fluorescent, making it an ideal probe for studying biological samples, detecting chemical compounds, and tracing fluid flow in various systems.
What are the benefits of using Fluorescein Sodium (C.I. 45350) for analysis?
Fluorescein Sodium offers several advantages for analytical purposes. Its intense fluorescence allows for sensitive detection of target molecules or structures in complex samples. Its stability and solubility in aqueous solutions make it easy to work with in a laboratory setting. Additionally, Fluorescein Sodium is relatively inexpensive and readily available, making it a cost-effective choice for research and analytical applications.
How does Fluorescein Sodium (C.I. 45350) work in fluorescence-based analysis techniques?
In fluorescence-based analysis techniques, Fluorescein Sodium is excited by a light source at a specific wavelength, causing it to fluoresce and emit light at a longer wavelength. This emitted fluorescence can be detected and quantified to provide information about the presence, concentration, or distribution of the target molecule or structure in the sample. By tailoring the excitation and emission wavelengths, researchers can selectively visualize specific components of interest in their samples.
What are some common applications of Fluorescein Sodium (C.I. 45350) in analytical chemistry?
Fluorescein Sodium is widely used in analytical chemistry for a variety of applications. It can be conjugated to antibodies or proteins to create fluorescently labeled probes for immunofluorescence assays and flow cytometry. It can also be used as a fluorescent tracer to visualize fluid flow in industrial processes or biological systems. Additionally, Fluorescein Sodium is utilized in environmental monitoring, pharmaceutical analysis, and cell biology research for its versatility and sensitivity.
How can researchers optimize their use of Fluorescein Sodium (C.I. 45350) for accurate and reliable analysis?
To achieve accurate and reliable results with Fluorescein Sodium, researchers should consider several factors. These include optimizing the excitation and emission settings of their fluorescence detection equipment, calibrating the instrument using known concentrations of Fluorescein Sodium standards, and carefully controlling experimental variables such as pH, temperature, and sample preparation. By following best practices for fluorescence-based analysis techniques, researchers can maximize the sensitivity and specificity of their assays using Fluorescein Sodium (C.I. 45350).
Fluorescein Sodium (C.I. 45350) is a synthetic organic compound commonly used in various analytical techniques such as fluorescence microscopy, flow cytometry, and spectrophotometry. It is highly fluorescent, making it an ideal probe for studying biological samples, detecting chemical compounds, and tracing fluid flow in various systems.
What are the benefits of using Fluorescein Sodium (C.I. 45350) for analysis?
Fluorescein Sodium offers several advantages for analytical purposes. Its intense fluorescence allows for sensitive detection of target molecules or structures in complex samples. Its stability and solubility in aqueous solutions make it easy to work with in a laboratory setting. Additionally, Fluorescein Sodium is relatively inexpensive and readily available, making it a cost-effective choice for research and analytical applications.
How does Fluorescein Sodium (C.I. 45350) work in fluorescence-based analysis techniques?
In fluorescence-based analysis techniques, Fluorescein Sodium is excited by a light source at a specific wavelength, causing it to fluoresce and emit light at a longer wavelength. This emitted fluorescence can be detected and quantified to provide information about the presence, concentration, or distribution of the target molecule or structure in the sample. By tailoring the excitation and emission wavelengths, researchers can selectively visualize specific components of interest in their samples.
What are some common applications of Fluorescein Sodium (C.I. 45350) in analytical chemistry?
Fluorescein Sodium is widely used in analytical chemistry for a variety of applications. It can be conjugated to antibodies or proteins to create fluorescently labeled probes for immunofluorescence assays and flow cytometry. It can also be used as a fluorescent tracer to visualize fluid flow in industrial processes or biological systems. Additionally, Fluorescein Sodium is utilized in environmental monitoring, pharmaceutical analysis, and cell biology research for its versatility and sensitivity.
How can researchers optimize their use of Fluorescein Sodium (C.I. 45350) for accurate and reliable analysis?
To achieve accurate and reliable results with Fluorescein Sodium, researchers should consider several factors. These include optimizing the excitation and emission settings of their fluorescence detection equipment, calibrating the instrument using known concentrations of Fluorescein Sodium standards, and carefully controlling experimental variables such as pH, temperature, and sample preparation. By following best practices for fluorescence-based analysis techniques, researchers can maximize the sensitivity and specificity of their assays using Fluorescein Sodium (C.I. 45350).
