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| Name | Fuchsin Basic (C.I. 42510) |
| Color | Red |
| Chemical Formula | C20H17N3Cl |
| Solubility | Soluble in water |
| Application | Clinical diagnosis |
| Use | Staining biological samples |
| Storage | Store in a cool, dry place |
| CAS Number | 632-99-5 |
| Molar Mass | 321.82 g/mol |
| Appearance | Powder |
| Purity | Minimum 95% |
| Stability | Stable under recommended storage conditions |
| Melting Point | 280-283°C |
| Boiling Point | Not available |
| Hazards | May cause irritation |
| Safety Precautions | Avoid contact with skin and eyes |
| Shelf Life | At least 2 years |
| Packaging | Available in different sized containers |
| Odor | Characteristic odor |
FAQ
What is Fuchsin Basic (C.I. 42510) and how is it used in clinical diagnosis?
Fuchsin Basic (C.I. 42510) is a synthetic dye that is commonly used in histology and clinical microbiology for staining biological samples. It is particularly useful in identifying acid-fast organisms such as Mycobacterium tuberculosis, the causative agent of tuberculosis. The bright pink color of Fuchsin Basic allows for easy visualization of these organisms under a microscope.
How does Fuchsin Basic (C.I. 42510) aid in the diagnosis of tuberculosis?
Fuchsin Basic (C.I. 42510) is an essential component of the Ziehl-Neelsen staining method, also known as the acid-fast stain, which is used to detect acid-fast bacteria like Mycobacterium tuberculosis. The dye penetrates the waxy cell wall of these organisms, allowing them to retain the pink color even after being exposed to acid-alcohol, which decolorizes other bacteria. This distinctive staining pattern helps in the accurate identification of tuberculosis bacteria in clinical samples.
What are the benefits of using Fuchsin Basic (C.I. 42510) for clinical diagnosis?
Fuchsin Basic (C.I. 42510) offers several advantages for clinical diagnosis, including its high sensitivity and specificity in detecting acid-fast bacteria. Its bright pink color provides clear contrast against the background, making it easier to identify and differentiate organisms of interest. Additionally, the staining process is relatively simple and fast, allowing for timely diagnosis and treatment of tuberculosis.
Are there any potential limitations or drawbacks to using Fuchsin Basic (C.I. 42510) in clinical diagnosis?
While Fuchsin Basic (C.I. 42510) is highly effective in staining acid-fast bacteria, it is essential to follow the staining protocol diligently to avoid false-positive or false-negative results. Overstaining or insufficient decolorization can lead to misinterpretation of the staining pattern, affecting the accuracy of diagnosis. Additionally, some laboratories may choose to use alternative staining methods or dyes based on the specific requirements of their testing procedures.
How can healthcare professionals ensure the proper use of Fuchsin Basic (C.I. 42510) for clinical diagnosis?
Healthcare professionals should be trained on the correct use of Fuchsin Basic (C.I. 42510) and familiarize themselves with the staining protocol recommended for detecting acid-fast bacteria. It is essential to handle the dye with care and follow strict safety measures to prevent any accidental exposure. Regular maintenance and quality control of staining equipment and reagents are also crucial to ensure reliable and reproducible results in clinical diagnosis.
Fuchsin Basic (C.I. 42510) is a synthetic dye that is commonly used in histology and clinical microbiology for staining biological samples. It is particularly useful in identifying acid-fast organisms such as Mycobacterium tuberculosis, the causative agent of tuberculosis. The bright pink color of Fuchsin Basic allows for easy visualization of these organisms under a microscope.
How does Fuchsin Basic (C.I. 42510) aid in the diagnosis of tuberculosis?
Fuchsin Basic (C.I. 42510) is an essential component of the Ziehl-Neelsen staining method, also known as the acid-fast stain, which is used to detect acid-fast bacteria like Mycobacterium tuberculosis. The dye penetrates the waxy cell wall of these organisms, allowing them to retain the pink color even after being exposed to acid-alcohol, which decolorizes other bacteria. This distinctive staining pattern helps in the accurate identification of tuberculosis bacteria in clinical samples.
What are the benefits of using Fuchsin Basic (C.I. 42510) for clinical diagnosis?
Fuchsin Basic (C.I. 42510) offers several advantages for clinical diagnosis, including its high sensitivity and specificity in detecting acid-fast bacteria. Its bright pink color provides clear contrast against the background, making it easier to identify and differentiate organisms of interest. Additionally, the staining process is relatively simple and fast, allowing for timely diagnosis and treatment of tuberculosis.
Are there any potential limitations or drawbacks to using Fuchsin Basic (C.I. 42510) in clinical diagnosis?
While Fuchsin Basic (C.I. 42510) is highly effective in staining acid-fast bacteria, it is essential to follow the staining protocol diligently to avoid false-positive or false-negative results. Overstaining or insufficient decolorization can lead to misinterpretation of the staining pattern, affecting the accuracy of diagnosis. Additionally, some laboratories may choose to use alternative staining methods or dyes based on the specific requirements of their testing procedures.
How can healthcare professionals ensure the proper use of Fuchsin Basic (C.I. 42510) for clinical diagnosis?
Healthcare professionals should be trained on the correct use of Fuchsin Basic (C.I. 42510) and familiarize themselves with the staining protocol recommended for detecting acid-fast bacteria. It is essential to handle the dye with care and follow strict safety measures to prevent any accidental exposure. Regular maintenance and quality control of staining equipment and reagents are also crucial to ensure reliable and reproducible results in clinical diagnosis.
