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| Name | SYBR Green® staining reagent, DNA free |
| Brand | Thermo Fisher Scientific |
| Type | Staining reagent |
| Composition | SYBR Green dye |
| Quantity | 500 µL |
| Concentration | 10,000X |
| Compatibility | DNA free |
| Application | DNA staining in gel electrophoresis |
| Excitation Wavelength | 488 nm |
| Emission Wavelength | 522 nm |
| Storage Conditions | Store at -20°C in the dark |
| Shelf Life | 1 year |
| Package Included | 1 tube of SYBR Green staining reagent, DNA free |
FAQ
What is SYBR Green® staining reagent?
SYBR Green® staining reagent is a versatile tool used in molecular biology to detect nucleic acids, particularly DNA. It is commonly used for visualizing and quantifying DNA in applications such as agarose gel electrophoresis, real-time PCR, and other nucleic acid detection assays.
Is SYBR Green® staining reagent DNA-free?
Yes, SYBR Green® staining reagent is specifically designed to be DNA-free, ensuring that it does not interfere with DNA samples being analyzed. This feature makes it an ideal choice for sensitive applications where DNA contamination could lead to misleading results.
How does SYBR Green® staining reagent work?
SYBR Green® staining reagent binds to double-stranded DNA by intercalating between the bases, resulting in a significant increase in fluorescence. This fluorescence can be visualized using a UV light source or a fluorescence imager, allowing researchers to analyze and quantify the DNA present in their samples.
What are the advantages of using SYBR Green® staining reagent?
SYBR Green® staining reagent offers several advantages, including high sensitivity, wide dynamic range, and low background fluorescence. It is also cost-effective and easy to use, making it a valuable tool for researchers looking to accurately quantify and visualize DNA in their experiments.
How can I incorporate SYBR Green® staining reagent into my research?
SYBR Green® staining reagent can be used in a variety of applications, including DNA electrophoresis, real-time PCR, and nucleic acid quantification assays. By following the recommended protocols and guidelines for each specific application, you can effectively utilize SYBR Green® staining reagent to enhance the quality and accuracy of your research.
SYBR Green® staining reagent is a versatile tool used in molecular biology to detect nucleic acids, particularly DNA. It is commonly used for visualizing and quantifying DNA in applications such as agarose gel electrophoresis, real-time PCR, and other nucleic acid detection assays.
Is SYBR Green® staining reagent DNA-free?
Yes, SYBR Green® staining reagent is specifically designed to be DNA-free, ensuring that it does not interfere with DNA samples being analyzed. This feature makes it an ideal choice for sensitive applications where DNA contamination could lead to misleading results.
How does SYBR Green® staining reagent work?
SYBR Green® staining reagent binds to double-stranded DNA by intercalating between the bases, resulting in a significant increase in fluorescence. This fluorescence can be visualized using a UV light source or a fluorescence imager, allowing researchers to analyze and quantify the DNA present in their samples.
What are the advantages of using SYBR Green® staining reagent?
SYBR Green® staining reagent offers several advantages, including high sensitivity, wide dynamic range, and low background fluorescence. It is also cost-effective and easy to use, making it a valuable tool for researchers looking to accurately quantify and visualize DNA in their experiments.
How can I incorporate SYBR Green® staining reagent into my research?
SYBR Green® staining reagent can be used in a variety of applications, including DNA electrophoresis, real-time PCR, and nucleic acid quantification assays. By following the recommended protocols and guidelines for each specific application, you can effectively utilize SYBR Green® staining reagent to enhance the quality and accuracy of your research.
