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| Name | Safranine O (C.I. 50240) |
| Type | clinical diagnosis product |
| Application | staining agent |
| Color | red |
| Chemical Formula | C20H19ClN4 |
| Molecular Weight | 350.84 g/mol |
| Solubility | soluble in water |
| Storage Condition | store in a cool, dry place |
| Purity | 98% |
| CAS Number | 477-73-6 |
| Pubchem CID | 5810 |
| Density | 1.3 g/cm3 |
| Melting Point | 265-266°C |
| Boiling Point | 568.9±50.0°C at 760 mmHg |
| Flash Point | 297.5±30.1°C |
| Usage | used in histology and clinical chemistry for staining biological samples |
FAQ
What is Safranine O (C.I. 50240) and how is it used for clinical diagnosis?
Safranine O (C.I. 50240) is a synthetic dye that is commonly used in histology and microbiology for staining tissues and bacteria. In clinical diagnosis, Safranine O is employed to aid in the detection of specific cells or structures in biological samples. By binding to certain components within the specimen, Safranine O can help highlight particular features under a microscope, allowing healthcare professionals to make more accurate diagnoses.
How does Safranine O work in clinical diagnosis?
Safranine O is a basic dye that has an affinity for acidic structures, such as nucleic acids and some proteins. In clinical diagnosis, Safranine O can be applied to tissue samples or specimens on a slide. The dye binds to specific targets within the sample, causing the structures of interest to take on a distinct color. This staining process enhances the visibility of the target structures, making it easier for healthcare professionals to identify and analyze them microscopically.
What are the benefits of using Safranine O for clinical diagnosis?
The use of Safranine O in clinical diagnosis offers several advantages. Firstly, the dye is highly versatile and can be used to stain a wide range of biological samples. Secondly, Safranine O produces clear and reliable staining results, aiding in the accurate interpretation of microscopic findings. Additionally, Safranine O staining is relatively simple and cost-effective, making it a valuable tool for diagnostic laboratories.
Are there any considerations or limitations when using Safranine O for clinical diagnosis?
While Safranine O is a valuable staining agent in clinical diagnosis, there are certain considerations to keep in mind. The dye may not be suitable for all types of samples or targets, as its specificity can vary. Additionally, over- or under-staining with Safranine O can affect the quality of the results obtained. It is essential for healthcare professionals to follow standardized protocols and best practices when using Safranine O for clinical diagnosis to ensure accurate and reliable outcomes.
How can healthcare professionals incorporate Safranine O into their clinical diagnostic workflows?
Healthcare professionals can incorporate Safranine O into their clinical diagnostic workflows by following established staining protocols and guidelines. Prior to using Safranine O, it is crucial to prepare the dye solution correctly and ensure that the sample is properly fixed and processed. By applying Safranine O according to recommended techniques, healthcare professionals can optimize the staining process and achieve consistent, high-quality results in clinical diagnosis.
By utilizing the staining capabilities of Safranine O, healthcare professionals can enhance their diagnostic capabilities and provide accurate and timely assessments of patient samples.
Safranine O (C.I. 50240) is a synthetic dye that is commonly used in histology and microbiology for staining tissues and bacteria. In clinical diagnosis, Safranine O is employed to aid in the detection of specific cells or structures in biological samples. By binding to certain components within the specimen, Safranine O can help highlight particular features under a microscope, allowing healthcare professionals to make more accurate diagnoses.
How does Safranine O work in clinical diagnosis?
Safranine O is a basic dye that has an affinity for acidic structures, such as nucleic acids and some proteins. In clinical diagnosis, Safranine O can be applied to tissue samples or specimens on a slide. The dye binds to specific targets within the sample, causing the structures of interest to take on a distinct color. This staining process enhances the visibility of the target structures, making it easier for healthcare professionals to identify and analyze them microscopically.
What are the benefits of using Safranine O for clinical diagnosis?
The use of Safranine O in clinical diagnosis offers several advantages. Firstly, the dye is highly versatile and can be used to stain a wide range of biological samples. Secondly, Safranine O produces clear and reliable staining results, aiding in the accurate interpretation of microscopic findings. Additionally, Safranine O staining is relatively simple and cost-effective, making it a valuable tool for diagnostic laboratories.
Are there any considerations or limitations when using Safranine O for clinical diagnosis?
While Safranine O is a valuable staining agent in clinical diagnosis, there are certain considerations to keep in mind. The dye may not be suitable for all types of samples or targets, as its specificity can vary. Additionally, over- or under-staining with Safranine O can affect the quality of the results obtained. It is essential for healthcare professionals to follow standardized protocols and best practices when using Safranine O for clinical diagnosis to ensure accurate and reliable outcomes.
How can healthcare professionals incorporate Safranine O into their clinical diagnostic workflows?
Healthcare professionals can incorporate Safranine O into their clinical diagnostic workflows by following established staining protocols and guidelines. Prior to using Safranine O, it is crucial to prepare the dye solution correctly and ensure that the sample is properly fixed and processed. By applying Safranine O according to recommended techniques, healthcare professionals can optimize the staining process and achieve consistent, high-quality results in clinical diagnosis.
By utilizing the staining capabilities of Safranine O, healthcare professionals can enhance their diagnostic capabilities and provide accurate and timely assessments of patient samples.
