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| Name | Thiazolyl Blue Tetrazolium Bromide (MTT) |
| Brand | BioChemica |
| Product Type | Biochemical |
| Form | Powder |
| Color | Yellow |
| Solubility | Soluble in water, DMSO, ethanol |
| Molecular Weight | 414.29 g/mol |
| Melting Point | 256-259°C |
| Storage Condition | Store at room temperature |
| Shelf Life | 2 years |
| Applications | Cell viability assay, cytotoxicity assay, cell proliferation assay |
| Cas Number | 298-93-1 |
| Pubchem Cid | 420660 |
| Smiles | CN1C=NC2=C1C(=O)N(C=C2)C3=CC(=C(C=C3)OC)Cl.[Br-] |
| Inchi | InChI=1S/C18H16N5O.BrH/c1-22-16-10-12-13(11-17(16)23(2)18(24)15(12)22)21-14-6-4-3-5-7-14;/h3-11,22H,1-2H3;1H/q+1;/p-1 |
FAQ
What is Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica?
MTT BioChemica is a chemical compound commonly used in biological assays to measure cell viability and proliferation. It is a yellow tetrazolium salt that is converted by living cells into a dark blue formazan product, allowing for the quantification of cell viability.
How is MTT BioChemica used in biological assays?
MTT BioChemica is typically added to cell cultures, where it is taken up and metabolized by viable cells. Metabolically active cells reduce MTT to formazan, which can be solubilized and quantified using spectrophotometric methods. This allows researchers to assess cell viability, proliferation, and cytotoxicity in a variety of experimental settings.
What are the advantages of using MTT BioChemica in cell viability assays?
MTT BioChemica offers several advantages for researchers conducting cell viability assays. It is a well-established and widely used method that is cost-effective and straightforward to perform. Additionally, MTT assays are sensitive, allowing for the detection of small changes in cell viability, making them ideal for studying the effects of various compounds and treatments on cell health.
Can MTT BioChemica be used in different cell types and experimental conditions?
Yes, MTT BioChemica is a versatile assay that can be used with a wide range of cell types and experimental conditions. It has been successfully employed in studies of cancer cells, stem cells, primary cells, and immortalized cell lines. Additionally, MTT assays can be modified to assess different endpoints, such as cell proliferation, cytotoxicity, and drug sensitivity.
Are there any limitations or considerations when using MTT BioChemica in biological assays?
While MTT BioChemica is a reliable and widely used assay, there are some limitations and considerations to keep in mind. Not all cells are capable of metabolizing MTT to formazan, so it is essential to validate the assay in specific cell types. Additionally, factors such as cell density, incubation time, and treatment conditions can affect the results of MTT assays, so careful optimization and controls are essential for accurate data interpretation.
MTT BioChemica is a chemical compound commonly used in biological assays to measure cell viability and proliferation. It is a yellow tetrazolium salt that is converted by living cells into a dark blue formazan product, allowing for the quantification of cell viability.
How is MTT BioChemica used in biological assays?
MTT BioChemica is typically added to cell cultures, where it is taken up and metabolized by viable cells. Metabolically active cells reduce MTT to formazan, which can be solubilized and quantified using spectrophotometric methods. This allows researchers to assess cell viability, proliferation, and cytotoxicity in a variety of experimental settings.
What are the advantages of using MTT BioChemica in cell viability assays?
MTT BioChemica offers several advantages for researchers conducting cell viability assays. It is a well-established and widely used method that is cost-effective and straightforward to perform. Additionally, MTT assays are sensitive, allowing for the detection of small changes in cell viability, making them ideal for studying the effects of various compounds and treatments on cell health.
Can MTT BioChemica be used in different cell types and experimental conditions?
Yes, MTT BioChemica is a versatile assay that can be used with a wide range of cell types and experimental conditions. It has been successfully employed in studies of cancer cells, stem cells, primary cells, and immortalized cell lines. Additionally, MTT assays can be modified to assess different endpoints, such as cell proliferation, cytotoxicity, and drug sensitivity.
Are there any limitations or considerations when using MTT BioChemica in biological assays?
While MTT BioChemica is a reliable and widely used assay, there are some limitations and considerations to keep in mind. Not all cells are capable of metabolizing MTT to formazan, so it is essential to validate the assay in specific cell types. Additionally, factors such as cell density, incubation time, and treatment conditions can affect the results of MTT assays, so careful optimization and controls are essential for accurate data interpretation.
