EC HPLC column EC 250/4.6 NUCLEOSIL 100-3 C18 length: 250 mm, ID: 4.6 mm

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Brand EC
Model HPLC column EC 250/4.6 NUCLEOSIL 100-3 C18
Length 250 mm
Diameter 4.6 mm
Particle Size 3 µm
Material C18
Packing Type Fully porous silica
Pore Size 100 Å
PH Range 2-8
Max Pressure 400 bar
Temperature Range up to 60°C
Analyte Type Organic compounds
Compatibility Acetonitrile, methanol, water
Applications HPLC, LC-MS
Surface Area 330 m²/g
Endcapping Yes
Carbon Load 18%
Description The EC 250/4.6 NUCLEOSIL 100-3 C18 HPLC column is a high-performance liquid chromatography column with a length of 250 mm, inner diameter of 4.6 mm, and particle size of 3 µm. It is packed with fully porous silica material (C18) with a pore size of 100 Å. The column is designed for separating organic compounds and is compatible with various solvents such as acetonitrile, methanol, and water. It has a pH range of 2-8, a maximum pressure of 400 bar, and can be operated at temperatures up to 60°C. The column is commonly used in HPLC and LC-MS applications, providing a surface area of 330 m²/g for efficient analyte separation. Additionally, the column is endcapped to minimize secondary interactions and has a carbon load of 18%.
FAQ
What is the EC HPLC column EC 250/4.6 NUCLEOSIL 100-3 C18?
The EC HPLC column EC 250/4.6 NUCLEOSIL 100-3 C18 is a high-performance liquid chromatography (HPLC) column with a length of 250 mm and an inner diameter of 4.6 mm. It is packed with NUCLEOSIL 100-3 C18 stationary phase material, which is known for its high efficiency and excellent peak shape.

What are the key features of the EC 250/4.6 NUCLEOSIL 100-3 C18 column?
The EC 250/4.6 NUCLEOSIL 100-3 C18 column offers high resolution and reproducibility, making it ideal for a wide range of HPLC applications. It has a long column length of 250 mm, which allows for better separation of complex mixtures. The 4.6 mm inner diameter ensures compatibility with most HPLC systems. The NUCLEOSIL 100-3 C18 stationary phase material provides excellent retention and separation of analytes.

What are the recommended applications for the EC 250/4.6 NUCLEOSIL 100-3 C18 column?
The EC 250/4.6 NUCLEOSIL 100-3 C18 column is suitable for the analysis of a variety of compounds, including pharmaceuticals, natural products, environmental samples, and more. It is commonly used for the separation of polar and non-polar compounds in complex mixtures. This column is well-suited for both analytical and preparative HPLC applications.

What are the benefits of using the EC 250/4.6 NUCLEOSIL 100-3 C18 column?
The EC 250/4.6 NUCLEOSIL 100-3 C18 column offers several benefits, including high efficiency, excellent peak shape, and good retention of analytes. It provides reproducible results and has a long lifespan, making it a cost-effective choice for HPLC applications. The NUCLEOSIL 100-3 C18 stationary phase material ensures consistent performance and reliable separations.

How should I care for and maintain the EC 250/4.6 NUCLEOSIL 100-3 C18 column?
To ensure optimal performance of the EC 250/4.6 NUCLEOSIL 100-3 C18 column, it is important to follow proper care and maintenance procedures. Always store the column in a clean and dry environment, away from direct sunlight and extreme temperatures. Before and after each use, flush the column with appropriate mobile phase to remove any contaminants. Regularly check for leaks, pressure fluctuations, and changes in retention times, which may indicate column damage or deterioration. In case of any issues, contact the manufacturer or supplier for further assistance.
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