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| Column Name | EC 250/4.6 NUCLEOSIL 50-5 C18 |
| Length | 250 mm |
| Inner Diameter | 4.6 mm |
| Packing Material | NUCLEOSIL 50-5 C18 |
| Pack Size | 1 product |
| Particle Size | 5 µm |
| Carbon Load | 18% |
| Pore Size | 100 Å |
| USP Classification | L1 |
| PH Range | 2-8 |
| Maximum Pressure | 400 bar |
| Compatibility | Reversed Phase |
| Recommended Applications | Pharmaceutical, Environmental, Food and Beverage Analysis |
| Brand | EC |
| Product Code | EC 250/4.6 NUCLEOSIL 50-5 C18 |
FAQ
What are the key features of the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18?
The EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18 features a length of 250 mm and an inner diameter of 4.6 mm. It is packed with a high-quality C18 stationary phase material that ensures excellent separation efficiency and peak symmetry. This column is designed for use in liquid chromatography applications, particularly in HPLC systems.
What are the benefits of using the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18?
Using the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18 offers several benefits, including:
- High separation efficiency: The C18 stationary phase provides excellent peak resolution for optimal chromatographic separation.
- Consistent performance: The column is designed for long-term stability and reproducibility, ensuring reliable results with each use.
- Wide compatibility: The column is compatible with a variety of solvents and mobile phases, making it versatile for different analytical applications.
- Cost-effective: The column comes in a pack of 1, offering value for money and reducing the need for frequent replacements.
- Easy to use: The column is designed for simple installation and operation, making it suitable for both experienced and novice chromatographers.
How can I optimize the performance of the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18?
To optimize the performance of the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18, consider the following tips:
- Use appropriate mobile phases: Select the right solvent system and gradient conditions to achieve the desired separation and resolution.
- Maintain proper column temperature: Ensure that the column is operated at the recommended temperature to prevent peak broadening and improve reproducibility.
- Regularly monitor column pressure: Check the backpressure of the column during operation to detect any potential issues and maintain peak sharpness.
- Use guard columns: Consider using a guard column to protect the analytical column from contaminants and extend its lifespan.
- Follow manufacturer's guidelines: Adhere to the recommended operating conditions and maintenance procedures provided by the column manufacturer to ensure optimal performance.
What is the recommended application of the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18?
The EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18 is suitable for a wide range of applications, including the separation and analysis of organic compounds, pharmaceuticals, natural products, environmental samples, and more. It is commonly used in research, quality control, and routine analysis laboratories for the detection and quantification of target analytes in complex sample matrices.
Can the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18 be used for method development and validation?
Yes, the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18 is well-suited for method development and validation in HPLC applications. Its high separation efficiency, reproducibility, and stability make it ideal for optimizing chromatographic conditions, establishing analytical methods, and validating the performance of the analytical system. By following standard guidelines and protocols, chromatographers can effectively develop and validate methods using this column to ensure accurate and reliable results.
The EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18 features a length of 250 mm and an inner diameter of 4.6 mm. It is packed with a high-quality C18 stationary phase material that ensures excellent separation efficiency and peak symmetry. This column is designed for use in liquid chromatography applications, particularly in HPLC systems.
What are the benefits of using the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18?
Using the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18 offers several benefits, including:
- High separation efficiency: The C18 stationary phase provides excellent peak resolution for optimal chromatographic separation.
- Consistent performance: The column is designed for long-term stability and reproducibility, ensuring reliable results with each use.
- Wide compatibility: The column is compatible with a variety of solvents and mobile phases, making it versatile for different analytical applications.
- Cost-effective: The column comes in a pack of 1, offering value for money and reducing the need for frequent replacements.
- Easy to use: The column is designed for simple installation and operation, making it suitable for both experienced and novice chromatographers.
How can I optimize the performance of the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18?
To optimize the performance of the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18, consider the following tips:
- Use appropriate mobile phases: Select the right solvent system and gradient conditions to achieve the desired separation and resolution.
- Maintain proper column temperature: Ensure that the column is operated at the recommended temperature to prevent peak broadening and improve reproducibility.
- Regularly monitor column pressure: Check the backpressure of the column during operation to detect any potential issues and maintain peak sharpness.
- Use guard columns: Consider using a guard column to protect the analytical column from contaminants and extend its lifespan.
- Follow manufacturer's guidelines: Adhere to the recommended operating conditions and maintenance procedures provided by the column manufacturer to ensure optimal performance.
What is the recommended application of the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18?
The EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18 is suitable for a wide range of applications, including the separation and analysis of organic compounds, pharmaceuticals, natural products, environmental samples, and more. It is commonly used in research, quality control, and routine analysis laboratories for the detection and quantification of target analytes in complex sample matrices.
Can the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18 be used for method development and validation?
Yes, the EC HPLC column EC 250/4.6 NUCLEOSIL 50-5 C18 is well-suited for method development and validation in HPLC applications. Its high separation efficiency, reproducibility, and stability make it ideal for optimizing chromatographic conditions, establishing analytical methods, and validating the performance of the analytical system. By following standard guidelines and protocols, chromatographers can effectively develop and validate methods using this column to ensure accurate and reliable results.
