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| Name | Silylation reagent BSTFA pack of 1x10 mL |
| Pack Size | 1x10 mL |
| Product Type | Silylation reagent |
| Total Volume | 10 mL |
| Reagent Type | BSTFA |
| Packaging | Glass vial |
| Application | Sample derivatization for gas chromatography (GC) |
| Purity | High purity |
| Storage Condition | Store at room temperature |
| Shelf Life | 2 years |
| Manufacturer | Not specified |
FAQ
What is BSTFA and how is it used in silylation reactions?
BSTFA (N,O-bis(trimethylsilyl)trifluoroacetamide) is a silylation reagent commonly used in analytical chemistry to derivatize polar compounds for analysis by gas chromatography (GC) or mass spectrometry (MS). It reacts with hydroxyl, carboxyl, and amino functional groups to form trimethylsilyl derivatives, which are more volatile and thermally stable, making them ideal for GC analysis.
How is BSTFA different from other silylation reagents?
BSTFA is preferred for silylation reactions due to its high reactivity, rapid derivatization, and stability of the resulting derivatives. It is known for its ability to form trimethylsilyl derivatives efficiently, even for compounds that are difficult to silylate with other reagents. BSTFA also has a relatively low vapor pressure, making it safe to handle in the laboratory.
What are the key advantages of using BSTFA in silylation reactions?
BSTFA offers several advantages in silylation reactions, including:
1. High reactivity: BSTFA reacts quickly with target functional groups, minimizing reaction times and improving efficiency.
2. Stability: BSTFA forms stable trimethylsilyl derivatives that are resistant to hydrolysis and thermal degradation, ensuring reliable results.
3. Versatility: BSTFA can be used for a wide range of compounds, including polar and non-polar molecules, making it a versatile reagent for silylation reactions.
4. Compatibility: BSTFA is compatible with a variety of solvents and additives commonly used in analytical chemistry, providing flexibility in experimental conditions.
5. Low background noise: BSTFA derivatives exhibit low background noise in GC and MS analyses, enhancing detection sensitivity and accuracy.
How should BSTFA be handled and stored to ensure optimal performance?
To maintain the quality and efficacy of BSTFA, it is important to follow proper handling and storage practices:
1. Storage: BSTFA should be stored in a cool, dry place away from direct sunlight and sources of heat to prevent degradation.
2. Handling: When handling BSTFA, wear appropriate personal protective equipment, including gloves and safety goggles, to protect against skin contact and inhalation.
3. Ventilation: Use BSTFA in a well-ventilated laboratory fume hood to minimize exposure to vapors and ensure a safe working environment.
4. Contamination: Avoid cross-contamination by using clean, dry glassware and tools for silylation reactions and storing BSTFA away from other reagents or chemicals.
5. Transport: When transporting BSTFA, ensure that the container is tightly sealed and securely packaged to prevent spills or leaks.
What are some common applications of BSTFA in analytical chemistry and research?
BSTFA is widely used in analytical chemistry and research for various applications, including:
1. Derivatization of amino acids, peptides, and proteins for GC-MS analysis.
2. Silylation of fatty acids, steroids, and other lipids to improve volatility and stability in GC.
3. Analysis of carbohydrates, sugars, and alcohols by silylation for GC-based detection.
4. Detection of environmental pollutants, pharmaceuticals, and metabolites in complex samples using GC-MS.
5. Identification of trace organic compounds in forensic, environmental, and food safety analyses.
Remember to consult the product's safety data sheet and technical documentation for detailed instructions on handling, storage, and disposal of BSTFA.
BSTFA (N,O-bis(trimethylsilyl)trifluoroacetamide) is a silylation reagent commonly used in analytical chemistry to derivatize polar compounds for analysis by gas chromatography (GC) or mass spectrometry (MS). It reacts with hydroxyl, carboxyl, and amino functional groups to form trimethylsilyl derivatives, which are more volatile and thermally stable, making them ideal for GC analysis.
How is BSTFA different from other silylation reagents?
BSTFA is preferred for silylation reactions due to its high reactivity, rapid derivatization, and stability of the resulting derivatives. It is known for its ability to form trimethylsilyl derivatives efficiently, even for compounds that are difficult to silylate with other reagents. BSTFA also has a relatively low vapor pressure, making it safe to handle in the laboratory.
What are the key advantages of using BSTFA in silylation reactions?
BSTFA offers several advantages in silylation reactions, including:
1. High reactivity: BSTFA reacts quickly with target functional groups, minimizing reaction times and improving efficiency.
2. Stability: BSTFA forms stable trimethylsilyl derivatives that are resistant to hydrolysis and thermal degradation, ensuring reliable results.
3. Versatility: BSTFA can be used for a wide range of compounds, including polar and non-polar molecules, making it a versatile reagent for silylation reactions.
4. Compatibility: BSTFA is compatible with a variety of solvents and additives commonly used in analytical chemistry, providing flexibility in experimental conditions.
5. Low background noise: BSTFA derivatives exhibit low background noise in GC and MS analyses, enhancing detection sensitivity and accuracy.
How should BSTFA be handled and stored to ensure optimal performance?
To maintain the quality and efficacy of BSTFA, it is important to follow proper handling and storage practices:
1. Storage: BSTFA should be stored in a cool, dry place away from direct sunlight and sources of heat to prevent degradation.
2. Handling: When handling BSTFA, wear appropriate personal protective equipment, including gloves and safety goggles, to protect against skin contact and inhalation.
3. Ventilation: Use BSTFA in a well-ventilated laboratory fume hood to minimize exposure to vapors and ensure a safe working environment.
4. Contamination: Avoid cross-contamination by using clean, dry glassware and tools for silylation reactions and storing BSTFA away from other reagents or chemicals.
5. Transport: When transporting BSTFA, ensure that the container is tightly sealed and securely packaged to prevent spills or leaks.
What are some common applications of BSTFA in analytical chemistry and research?
BSTFA is widely used in analytical chemistry and research for various applications, including:
1. Derivatization of amino acids, peptides, and proteins for GC-MS analysis.
2. Silylation of fatty acids, steroids, and other lipids to improve volatility and stability in GC.
3. Analysis of carbohydrates, sugars, and alcohols by silylation for GC-based detection.
4. Detection of environmental pollutants, pharmaceuticals, and metabolites in complex samples using GC-MS.
5. Identification of trace organic compounds in forensic, environmental, and food safety analyses.
Remember to consult the product's safety data sheet and technical documentation for detailed instructions on handling, storage, and disposal of BSTFA.
