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| Name | Ammonium Sulfate |
| Category | Chemical compound |
| Formula | (NH4)2SO4 |
| Molecular Weight | 132.14 g/mol |
| Appearance | White crystalline solid |
| Solubility | Soluble in water |
| Purity | Minimum 99.0% |
| Storage Condition | Store at room temperature |
| Usage | Commonly used for protein purification and precipitation in molecular biology |
| Shelf Life | Indefinite if stored properly |
| CAS Number | 7783-20-2 |
| EC Number | 231-984-1 |
FAQ
What is Ammonium Sulfate and how is it used in molecular biology?
Ammonium Sulfate is a commonly used reagent in molecular biology for protein purification. It is a white, crystalline solid that is highly soluble in water and separates easily from aqueous solutions, making it ideal for precipitation and purification processes. Ammonium Sulfate is often used to fractionate proteins based on their solubility.
Why is Ammonium Sulfate preferred for protein purification?
Ammonium Sulfate is preferred for protein purification because of its ability to selectively precipitate proteins based on their solubility at different concentrations of the salt. This allows for the separation of proteins from contaminants and other impurities in a protein sample. Additionally, Ammonium Sulfate is cost-effective, readily available, and easy to use, making it a popular choice for protein purification experiments.
How does Ammonium Sulfate work in protein purification?
Ammonium Sulfate works by altering the solubility of proteins in solution. As the concentration of Ammonium Sulfate in the solution increases, the solubility of proteins decreases. This leads to the precipitation of proteins at specific concentrations of Ammonium Sulfate, allowing for their separation from the solution. The precipitated proteins can then be collected and further purified using other techniques.
What are the benefits of using Ammonium Sulfate for protein purification?
There are several benefits to using Ammonium Sulfate for protein purification. Firstly, it is a versatile reagent that can be used to precipitate a wide range of proteins. Secondly, Ammonium Sulfate is easy to use and does not require specialized equipment or expertise. Additionally, it is cost-effective and readily available, making it suitable for both small-scale and large-scale protein purification experiments. Lastly, Ammonium Sulfate allows for the selective precipitation of proteins, resulting in high purity and yield of the target protein.
Are there any potential limitations or considerations when using Ammonium Sulfate for protein purification?
While Ammonium Sulfate is an effective reagent for protein purification, there are some limitations and considerations to keep in mind. One potential limitation is that some proteins may not precipitate efficiently or selectively with Ammonium Sulfate, requiring alternative purification methods. Additionally, care must be taken when handling Ammonium Sulfate as it can be corrosive and irritate the skin and eyes. It is important to use appropriate personal protective equipment and follow safety guidelines when working with this reagent.
Ammonium Sulfate is a commonly used reagent in molecular biology for protein purification. It is a white, crystalline solid that is highly soluble in water and separates easily from aqueous solutions, making it ideal for precipitation and purification processes. Ammonium Sulfate is often used to fractionate proteins based on their solubility.
Why is Ammonium Sulfate preferred for protein purification?
Ammonium Sulfate is preferred for protein purification because of its ability to selectively precipitate proteins based on their solubility at different concentrations of the salt. This allows for the separation of proteins from contaminants and other impurities in a protein sample. Additionally, Ammonium Sulfate is cost-effective, readily available, and easy to use, making it a popular choice for protein purification experiments.
How does Ammonium Sulfate work in protein purification?
Ammonium Sulfate works by altering the solubility of proteins in solution. As the concentration of Ammonium Sulfate in the solution increases, the solubility of proteins decreases. This leads to the precipitation of proteins at specific concentrations of Ammonium Sulfate, allowing for their separation from the solution. The precipitated proteins can then be collected and further purified using other techniques.
What are the benefits of using Ammonium Sulfate for protein purification?
There are several benefits to using Ammonium Sulfate for protein purification. Firstly, it is a versatile reagent that can be used to precipitate a wide range of proteins. Secondly, Ammonium Sulfate is easy to use and does not require specialized equipment or expertise. Additionally, it is cost-effective and readily available, making it suitable for both small-scale and large-scale protein purification experiments. Lastly, Ammonium Sulfate allows for the selective precipitation of proteins, resulting in high purity and yield of the target protein.
Are there any potential limitations or considerations when using Ammonium Sulfate for protein purification?
While Ammonium Sulfate is an effective reagent for protein purification, there are some limitations and considerations to keep in mind. One potential limitation is that some proteins may not precipitate efficiently or selectively with Ammonium Sulfate, requiring alternative purification methods. Additionally, care must be taken when handling Ammonium Sulfate as it can be corrosive and irritate the skin and eyes. It is important to use appropriate personal protective equipment and follow safety guidelines when working with this reagent.
