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| Name | Guanidine Hydrochloride |
| Category | Chemical compound |
| Applications | Molecular biology, protein denaturation, RNA extraction |
| Purity | 98% |
| Form | White crystalline powder |
| Solubility | Soluble in water |
| Storage | Store at room temperature |
| Molecular Weight | 95.53 g/mol |
| Boiling Point | 186°C |
| Melting Point | 185-189°C |
| Cas Number | 50-01-1 |
| Molar Mass | 95.527 g/mol |
FAQ
What is Guanidine Hydrochloride and how is it used in molecular biology?
Guanidine Hydrochloride is a chaotropic agent commonly used in molecular biology for various applications. It is primarily used to denature proteins by disrupting hydrogen bonding and disrupting hydrophobic interactions. This allows for efficient extraction of DNA, RNA, and proteins from biological samples.
Can Guanidine Hydrochloride be used for RNA isolation?
Yes, Guanidine Hydrochloride is commonly used for RNA isolation. It helps in the denaturation of proteins and RNA binding proteins, allowing for the efficient extraction of RNA from biological samples. Additionally, it helps in the inactivation of RNases, which are enzymes that degrade RNA.
How does Guanidine Hydrochloride aid in protein purification?
Guanidine Hydrochloride is often used in protein purification protocols to denature proteins and disrupt protein-protein interactions. This helps in solubilizing proteins and allows for their purification by techniques such as chromatography. Guanidine Hydrochloride is preferred over other denaturing agents due to its chaotropic properties.
What are the advantages of using Guanidine Hydrochloride over other chaotropic agents in molecular biology?
Guanidine Hydrochloride offers several advantages over other chaotropic agents in molecular biology. It is highly soluble in water, making it easy to prepare solutions of varying concentrations. It is also relatively inexpensive and readily available, making it a cost-effective choice for labs. Additionally, Guanidine Hydrochloride is stable at room temperature, which allows for convenient storage.
Can Guanidine Hydrochloride be used for cell lysis in molecular biology experiments?
Yes, Guanidine Hydrochloride can be used for cell lysis in molecular biology experiments. It helps in breaking down cell membranes and denaturing proteins, releasing cellular components such as DNA, RNA, and proteins. Guanidine Hydrochloride is commonly used in protocols for nucleic acid isolation and protein extraction from cells.
Guanidine Hydrochloride is a chaotropic agent commonly used in molecular biology for various applications. It is primarily used to denature proteins by disrupting hydrogen bonding and disrupting hydrophobic interactions. This allows for efficient extraction of DNA, RNA, and proteins from biological samples.
Can Guanidine Hydrochloride be used for RNA isolation?
Yes, Guanidine Hydrochloride is commonly used for RNA isolation. It helps in the denaturation of proteins and RNA binding proteins, allowing for the efficient extraction of RNA from biological samples. Additionally, it helps in the inactivation of RNases, which are enzymes that degrade RNA.
How does Guanidine Hydrochloride aid in protein purification?
Guanidine Hydrochloride is often used in protein purification protocols to denature proteins and disrupt protein-protein interactions. This helps in solubilizing proteins and allows for their purification by techniques such as chromatography. Guanidine Hydrochloride is preferred over other denaturing agents due to its chaotropic properties.
What are the advantages of using Guanidine Hydrochloride over other chaotropic agents in molecular biology?
Guanidine Hydrochloride offers several advantages over other chaotropic agents in molecular biology. It is highly soluble in water, making it easy to prepare solutions of varying concentrations. It is also relatively inexpensive and readily available, making it a cost-effective choice for labs. Additionally, Guanidine Hydrochloride is stable at room temperature, which allows for convenient storage.
Can Guanidine Hydrochloride be used for cell lysis in molecular biology experiments?
Yes, Guanidine Hydrochloride can be used for cell lysis in molecular biology experiments. It helps in breaking down cell membranes and denaturing proteins, releasing cellular components such as DNA, RNA, and proteins. Guanidine Hydrochloride is commonly used in protocols for nucleic acid isolation and protein extraction from cells.
