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Name | Guanidine Thiocyanate 6 mol/l (6 M in 0.1 M Tris; pH 7.5) |
Category | Molecular Biology Product |
Concentration | 6 mol/l |
PH | 7.5 |
Buffer | 0.1 M Tris |
Storage Condition | Store at room temperature |
Appearance | Clear liquid |
Solubility | Soluble in water |
Usage | Commonly used in nucleic acid isolation and purification procedures |
Stability | Stable for long term storage |
Molecular Weight | 118.16 g/mol |
Boiling Point | 186°C |
Melting Point | 116-118°C |
Flash Point | 136°C |
Density | 1.6 g/cm3 |
Chemical Formula | C2H6N4S |
Cas Number | 593-84-0 |
FAQ
What is Guanidine Thiocyanate 6 mol/L and how is it used in molecular biology?
Guanidine Thiocyanate is a chaotropic agent commonly used in molecular biology to disrupt protein structures and denature proteins. It is often used in conjunction with other reagents to isolate RNA, DNA, and proteins from biological samples.
What are the advantages of using Guanidine Thiocyanate 6 mol/L in molecular biology experiments?
Guanidine Thiocyanate has several advantages in molecular biology applications. It effectively denatures proteins, disrupts protein structures, and helps in the isolation of RNA, DNA, and proteins. It also helps to stabilize RNA and inhibit RNase activity, making it an ideal reagent for RNA extraction.
How should Guanidine Thiocyanate 6 mol/L be stored and handled in the laboratory?
Guanidine Thiocyanate should be stored at room temperature or below in a dry, well-ventilated area. It should be handled with care, using appropriate personal protective equipment such as gloves and goggles. The reagent should be kept away from heat, sparks, and open flames. It is also important to avoid contact with skin, eyes, and clothing, and to wash hands thoroughly after handling.
What safety precautions should be taken when working with Guanidine Thiocyanate 6 mol/L in the laboratory?
When working with Guanidine Thiocyanate, it is important to follow all safety precautions outlined in the material safety data sheet (MSDS). Wear appropriate personal protective equipment, including gloves, goggles, and lab coat. Avoid contact with skin, eyes, and clothing, and do not ingest or inhale the reagent. In case of contact, rinse affected area with plenty of water and seek medical attention if necessary.
Can Guanidine Thiocyanate 6 mol/L be used in conjunction with other reagents for molecular biology experiments?
Yes, Guanidine Thiocyanate can be used in conjunction with other reagents for various molecular biology experiments. It is often used in combination with phenol-chloroform extraction, ethanol precipitation, and other purification techniques to isolate nucleic acids and proteins from complex biological samples. Additionally, it can be used in RNA extraction kits and protocols for efficient RNA isolation.
Guanidine Thiocyanate is a chaotropic agent commonly used in molecular biology to disrupt protein structures and denature proteins. It is often used in conjunction with other reagents to isolate RNA, DNA, and proteins from biological samples.
What are the advantages of using Guanidine Thiocyanate 6 mol/L in molecular biology experiments?
Guanidine Thiocyanate has several advantages in molecular biology applications. It effectively denatures proteins, disrupts protein structures, and helps in the isolation of RNA, DNA, and proteins. It also helps to stabilize RNA and inhibit RNase activity, making it an ideal reagent for RNA extraction.
How should Guanidine Thiocyanate 6 mol/L be stored and handled in the laboratory?
Guanidine Thiocyanate should be stored at room temperature or below in a dry, well-ventilated area. It should be handled with care, using appropriate personal protective equipment such as gloves and goggles. The reagent should be kept away from heat, sparks, and open flames. It is also important to avoid contact with skin, eyes, and clothing, and to wash hands thoroughly after handling.
What safety precautions should be taken when working with Guanidine Thiocyanate 6 mol/L in the laboratory?
When working with Guanidine Thiocyanate, it is important to follow all safety precautions outlined in the material safety data sheet (MSDS). Wear appropriate personal protective equipment, including gloves, goggles, and lab coat. Avoid contact with skin, eyes, and clothing, and do not ingest or inhale the reagent. In case of contact, rinse affected area with plenty of water and seek medical attention if necessary.
Can Guanidine Thiocyanate 6 mol/L be used in conjunction with other reagents for molecular biology experiments?
Yes, Guanidine Thiocyanate can be used in conjunction with other reagents for various molecular biology experiments. It is often used in combination with phenol-chloroform extraction, ethanol precipitation, and other purification techniques to isolate nucleic acids and proteins from complex biological samples. Additionally, it can be used in RNA extraction kits and protocols for efficient RNA isolation.