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| Name | Guanidine Thiocyanate |
| Product Type | Molecular biology reagent |
| Chemical Formula | CH4N2S |
| Molecular Weight | 118.16 g/mol |
| Appearance | White crystalline powder |
| Solubility | Soluble in water |
| Purity | ≥ 99% |
| Storage Conditions | Store at room temperature |
| Usage | Commonly used for nucleic acid extraction and purification |
| Benefits | Denatures proteins and dissolves cell membranes |
| Applications | RNA isolation, DNA extraction, PCR |
| Risk Phrases | Harmful if swallowed, causes skin irritation, may cause respiratory irritation |
| Safety Precautions | Avoid inhalation, ingestion, and contact with skin and eyes |
| Handling Precautions | Use in a well-ventilated area and wear appropriate protective clothing |
FAQ
What is Guanidine Thiocyanate and how is it used in molecular biology?
Guanidine Thiocyanate is a powerful denaturant commonly used in molecular biology applications. It is often used in nucleic acid extraction protocols to disrupt the structure of proteins and RNA, allowing for the isolation of high-quality DNA. Guanidine Thiocyanate is also used in RNA extraction methods, as it helps solubilize RNA and inhibit RNase enzymes that can degrade nucleic acids.
How does Guanidine Thiocyanate compare to other denaturants in molecular biology applications?
Guanidine Thiocyanate is known for its ability to completely denature proteins and RNA, making it a popular choice in molecular biology applications. Compared to other denaturants like Guanidine Hydrochloride or urea, Guanidine Thiocyanate is often preferred for its efficiency and reliability in nucleic acid extraction protocols.
What are some key benefits of using Guanidine Thiocyanate in molecular biology experiments?
Some key benefits of using Guanidine Thiocyanate in molecular biology experiments include its ability to efficiently denature proteins and RNA, its compatibility with a wide range of nucleic acid extraction protocols, and its effectiveness in inhibiting RNase enzymes. Additionally, Guanidine Thiocyanate is known for its stability and long shelf life, making it a reliable reagent for laboratory use.
Are there any specific precautions or considerations to keep in mind when working with Guanidine Thiocyanate?
When working with Guanidine Thiocyanate, it is important to wear appropriate personal protective equipment, such as gloves and lab coats, to prevent skin contact. Additionally, Guanidine Thiocyanate should be handled in a well-ventilated area to avoid inhalation of fumes. It is also important to store Guanidine Thiocyanate in a cool, dry place away from direct sunlight to maintain its stability and effectiveness.
How can Guanidine Thiocyanate be integrated into different molecular biology protocols?
Guanidine Thiocyanate can be incorporated into a variety of molecular biology protocols, including nucleic acid extraction, RNA isolation, and protein denaturation. It can be used in conjunction with other reagents and techniques to achieve optimal results in experiments such as PCR, sequencing, and cloning. Its versatility and compatibility make it a valuable tool in the molecular biology laboratory.
Guanidine Thiocyanate is a powerful denaturant commonly used in molecular biology applications. It is often used in nucleic acid extraction protocols to disrupt the structure of proteins and RNA, allowing for the isolation of high-quality DNA. Guanidine Thiocyanate is also used in RNA extraction methods, as it helps solubilize RNA and inhibit RNase enzymes that can degrade nucleic acids.
How does Guanidine Thiocyanate compare to other denaturants in molecular biology applications?
Guanidine Thiocyanate is known for its ability to completely denature proteins and RNA, making it a popular choice in molecular biology applications. Compared to other denaturants like Guanidine Hydrochloride or urea, Guanidine Thiocyanate is often preferred for its efficiency and reliability in nucleic acid extraction protocols.
What are some key benefits of using Guanidine Thiocyanate in molecular biology experiments?
Some key benefits of using Guanidine Thiocyanate in molecular biology experiments include its ability to efficiently denature proteins and RNA, its compatibility with a wide range of nucleic acid extraction protocols, and its effectiveness in inhibiting RNase enzymes. Additionally, Guanidine Thiocyanate is known for its stability and long shelf life, making it a reliable reagent for laboratory use.
Are there any specific precautions or considerations to keep in mind when working with Guanidine Thiocyanate?
When working with Guanidine Thiocyanate, it is important to wear appropriate personal protective equipment, such as gloves and lab coats, to prevent skin contact. Additionally, Guanidine Thiocyanate should be handled in a well-ventilated area to avoid inhalation of fumes. It is also important to store Guanidine Thiocyanate in a cool, dry place away from direct sunlight to maintain its stability and effectiveness.
How can Guanidine Thiocyanate be integrated into different molecular biology protocols?
Guanidine Thiocyanate can be incorporated into a variety of molecular biology protocols, including nucleic acid extraction, RNA isolation, and protein denaturation. It can be used in conjunction with other reagents and techniques to achieve optimal results in experiments such as PCR, sequencing, and cloning. Its versatility and compatibility make it a valuable tool in the molecular biology laboratory.
