Back
| Name | Leupeptin Hemisulfate |
| Cas Number | 103476-89-7 |
| Molecular Formula | C20H38N6O4S |
| Molecular Weight | 464.63 g/mol |
| Appearance | White to off-white solid |
| Solubility | Soluble in water |
| Storage Condition | Store at -20°C |
| Purity | ≥98% |
| Usage | Protease inhibitor |
| Specific Activity | ≥100,000 CU/mg |
| Source | Synthetic origin |
| Shelf Life | 2 years |
| Pubchem Id | 4330 |
| MdL Number | MFCD00082200 |
| Availability | In stock |
| Shipping Restrictions | Ships worldwide |
FAQ
What is Leupeptin Hemisulfate and how does it work in biological research?
Leupeptin is a reversible inhibitor of cysteine and serine proteases. It is commonly used in biological research to inhibit proteolysis and prevent protein degradation. Leupeptin Hemisulfate, a salt form of leupeptin, is often used to increase the stability of proteins in cell lysates and tissue extracts.
What are the main applications of Leupeptin Hemisulfate in research?
Leupeptin Hemisulfate is widely used in research applications such as protein purification, western blotting, enzyme inhibition studies, and cell culture. It can help researchers to preserve the integrity of proteins during various experimental procedures and prevent unwanted protein degradation.
What are the benefits of using Leupeptin Hemisulfate in protein purification?
When purifying proteins from complex biological samples, proteases can degrade the target protein, leading to poor yield and compromised purity. By adding Leupeptin Hemisulfate to the purification buffer, researchers can prevent proteolysis and improve the overall efficiency of the purification process.
How does Leupeptin Hemisulfate compare to other protease inhibitors available on the market?
Leupeptin Hemisulfate is known for its broad-spectrum activity against a wide range of proteases, making it a versatile choice for researchers working with different types of proteins. Its reversible inhibition mechanism allows researchers to control the activity of proteases in a precise manner, enhancing the reproducibility of experimental results.
What are some best practices for using Leupeptin Hemisulfate in biological research?
To ensure the effectiveness of Leupeptin Hemisulfate, researchers should store the compound at -20°C and protect it from exposure to light and moisture. It is recommended to prepare fresh working solutions of Leupeptin Hemisulfate before each experiment and avoid repeated freeze-thaw cycles to maintain its stability.
Leupeptin is a reversible inhibitor of cysteine and serine proteases. It is commonly used in biological research to inhibit proteolysis and prevent protein degradation. Leupeptin Hemisulfate, a salt form of leupeptin, is often used to increase the stability of proteins in cell lysates and tissue extracts.
What are the main applications of Leupeptin Hemisulfate in research?
Leupeptin Hemisulfate is widely used in research applications such as protein purification, western blotting, enzyme inhibition studies, and cell culture. It can help researchers to preserve the integrity of proteins during various experimental procedures and prevent unwanted protein degradation.
What are the benefits of using Leupeptin Hemisulfate in protein purification?
When purifying proteins from complex biological samples, proteases can degrade the target protein, leading to poor yield and compromised purity. By adding Leupeptin Hemisulfate to the purification buffer, researchers can prevent proteolysis and improve the overall efficiency of the purification process.
How does Leupeptin Hemisulfate compare to other protease inhibitors available on the market?
Leupeptin Hemisulfate is known for its broad-spectrum activity against a wide range of proteases, making it a versatile choice for researchers working with different types of proteins. Its reversible inhibition mechanism allows researchers to control the activity of proteases in a precise manner, enhancing the reproducibility of experimental results.
What are some best practices for using Leupeptin Hemisulfate in biological research?
To ensure the effectiveness of Leupeptin Hemisulfate, researchers should store the compound at -20°C and protect it from exposure to light and moisture. It is recommended to prepare fresh working solutions of Leupeptin Hemisulfate before each experiment and avoid repeated freeze-thaw cycles to maintain its stability.
