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| Name | Putrescine dihydrochloride |
| Formula | C4H14Cl2N2 |
| Molar Mass | 167.08 g/mol |
| Appearance | White crystalline powder |
| Solubility | Soluble in water |
| Melting Point | 190-193°C |
| Boiling Point | 159-163°C |
| Density | 1.16 g/cm3 |
| Purity | ≥98% |
| Storage Conditions | Store in a cool, dry place |
| Hazard Warnings | Harmful if swallowed, irritating to skin and eyes |
FAQ
What is putrescine dihydrochloride and how is it used?
Putrescine dihydrochloride is a compound that is commonly used in the field of molecular biology and biochemistry. It is a derivative of putrescine, a polyamine that plays an important role in cell growth and proliferation. Putrescine dihydrochloride is often used as a chemical reagent in laboratory settings for various applications, including cell culture, DNA/RNA extraction, and protein purification.
How does putrescine dihydrochloride work in molecular biology experiments?
Putrescine dihydrochloride works by regulating the activity of enzymes involved in DNA replication, transcription, and translation. It can also stabilize nucleic acids and proteins, making it a valuable tool for researchers studying gene expression, protein synthesis, and other cellular processes. Additionally, putrescine dihydrochloride can aid in the formation of polyamine complexes that interact with DNA and RNA molecules, influencing their structure and function.
What are some common applications of putrescine dihydrochloride in research?
Putrescine dihydrochloride is widely used in molecular biology and biochemistry research for a variety of purposes. Some common applications include:
- Cell culture: Putrescine dihydrochloride can be added to cell culture media to promote cell growth and viability, particularly in fast-dividing cell lines.
- DNA and RNA extraction: Putrescine dihydrochloride can help to stabilize nucleic acids during extraction procedures, resulting in higher yields of pure DNA and RNA.
- Protein purification: Putrescine dihydrochloride can be used as a stabilizing agent during protein purification processes, improving the overall yield and quality of the final protein product.
- Enzyme assays: Putrescine dihydrochloride is often included in enzyme assay buffers to enhance the activity and stability of enzymes, leading to more accurate and reliable results.
- Gene expression studies: Putrescine dihydrochloride can influence the expression of certain genes by modulating the activity of transcription factors and other regulatory proteins, making it useful for studying gene regulation.
Overall, putrescine dihydrochloride is a versatile chemical reagent that has numerous applications in molecular biology research.
What are the advantages of using putrescine dihydrochloride in laboratory experiments?
There are several advantages to using putrescine dihydrochloride in laboratory experiments, including:
- Enhanced cell growth: Putrescine dihydrochloride can promote cell growth and proliferation in cell culture systems, making it easier to maintain and expand cell lines.
- Increased nucleic acid stability: Putrescine dihydrochloride can help to stabilize DNA and RNA molecules during extraction and purification procedures, leading to higher yields of intact nucleic acids.
- Improved protein stability: Putrescine dihydrochloride can enhance the stability of proteins during purification and storage, reducing the risk of denaturation and degradation.
- Enhanced enzyme activity: Putrescine dihydrochloride can improve the activity and efficiency of enzymes in biochemical assays, resulting in more sensitive and reliable measurements.
- Modulation of gene expression: Putrescine dihydrochloride can influence the expression of specific genes by interacting with regulatory proteins, providing researchers with a tool to study gene regulation mechanisms.
Overall, the use of putrescine dihydrochloride can help researchers achieve better results in their experiments by enhancing cell growth, stabilizing nucleic acids and proteins, improving enzyme activity, and modulating gene expression.
Putrescine dihydrochloride is a compound that is commonly used in the field of molecular biology and biochemistry. It is a derivative of putrescine, a polyamine that plays an important role in cell growth and proliferation. Putrescine dihydrochloride is often used as a chemical reagent in laboratory settings for various applications, including cell culture, DNA/RNA extraction, and protein purification.
How does putrescine dihydrochloride work in molecular biology experiments?
Putrescine dihydrochloride works by regulating the activity of enzymes involved in DNA replication, transcription, and translation. It can also stabilize nucleic acids and proteins, making it a valuable tool for researchers studying gene expression, protein synthesis, and other cellular processes. Additionally, putrescine dihydrochloride can aid in the formation of polyamine complexes that interact with DNA and RNA molecules, influencing their structure and function.
What are some common applications of putrescine dihydrochloride in research?
Putrescine dihydrochloride is widely used in molecular biology and biochemistry research for a variety of purposes. Some common applications include:
- Cell culture: Putrescine dihydrochloride can be added to cell culture media to promote cell growth and viability, particularly in fast-dividing cell lines.
- DNA and RNA extraction: Putrescine dihydrochloride can help to stabilize nucleic acids during extraction procedures, resulting in higher yields of pure DNA and RNA.
- Protein purification: Putrescine dihydrochloride can be used as a stabilizing agent during protein purification processes, improving the overall yield and quality of the final protein product.
- Enzyme assays: Putrescine dihydrochloride is often included in enzyme assay buffers to enhance the activity and stability of enzymes, leading to more accurate and reliable results.
- Gene expression studies: Putrescine dihydrochloride can influence the expression of certain genes by modulating the activity of transcription factors and other regulatory proteins, making it useful for studying gene regulation.
Overall, putrescine dihydrochloride is a versatile chemical reagent that has numerous applications in molecular biology research.
What are the advantages of using putrescine dihydrochloride in laboratory experiments?
There are several advantages to using putrescine dihydrochloride in laboratory experiments, including:
- Enhanced cell growth: Putrescine dihydrochloride can promote cell growth and proliferation in cell culture systems, making it easier to maintain and expand cell lines.
- Increased nucleic acid stability: Putrescine dihydrochloride can help to stabilize DNA and RNA molecules during extraction and purification procedures, leading to higher yields of intact nucleic acids.
- Improved protein stability: Putrescine dihydrochloride can enhance the stability of proteins during purification and storage, reducing the risk of denaturation and degradation.
- Enhanced enzyme activity: Putrescine dihydrochloride can improve the activity and efficiency of enzymes in biochemical assays, resulting in more sensitive and reliable measurements.
- Modulation of gene expression: Putrescine dihydrochloride can influence the expression of specific genes by interacting with regulatory proteins, providing researchers with a tool to study gene regulation mechanisms.
Overall, the use of putrescine dihydrochloride can help researchers achieve better results in their experiments by enhancing cell growth, stabilizing nucleic acids and proteins, improving enzyme activity, and modulating gene expression.
