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| Product Name | Tetrabutylammonium Hydrogen Sulfate for HPLC |
| CAS Number | 32503-27-8 |
| Molecular Formula | C16H36N.HSO4 |
| Molecular Weight | 325.50 g/mol |
| Appearance | White to off-white powder |
| Purity | ≥99% |
| Solubility | Soluble in water and organic solvents |
| Storage Conditions | Store in a cool, dry place |
| Shelf Life | 2 years |
| Usage | Used as a phase-transfer catalyst in HPLC |
| Melting Point | 140-145°C |
| Boiling Point | Not available |
| Density | Not available |
| Safety Information | Avoid contact with skin and eyes. Wear appropriate protective clothing and gloves |
FAQ
What is Tetrabutylammonium Hydrogen Sulfate (TBAHS) and how is it used in
HPLC?
Tetrabutylammonium Hydrogen Sulfate (TBAHS) is a salt compound that is commonly used as an ion pairing reagent in High-Performance Liquid Chromatography (HPLC) analyses. It is typically used in reverse-phase chromatography to improve the resolution and retention of charged, water-soluble compounds by forming ion pairs with them. This allows for better separation and identification of analytes in complex sample matrices.
What are the benefits of using Tetrabutylammonium Hydrogen Sulfate in HPLC?
One of the main benefits of using Tetrabutylammonium Hydrogen Sulfate in HPLC is its ability to enhance the separation of charged compounds, particularly those that are highly polar or hydrophilic. By forming ion pairs with these compounds, TBAHS helps to improve their retention on the column and reduce their interactions with the stationary phase, resulting in sharper peaks and better resolution. Additionally, TBAHS can also improve the sensitivity and reproducibility of HPLC analyses, making it an invaluable tool for researchers and analysts working with challenging samples.
How is Tetrabutylammonium Hydrogen Sulfate typically added to HPLC mobile phases?
Tetrabutylammonium Hydrogen Sulfate is usually added to the mobile phase in HPLC as a solution in an organic solvent such as methanol or acetonitrile. The concentration of TBAHS in the mobile phase can vary depending on the specific application and the analytes being separated, but is typically in the range of 0.1-10 mM. It is important to ensure that the TBAHS solution is properly mixed with the mobile phase before use to ensure uniform distribution throughout the system.
Are there any considerations or precautions to keep in mind when using Tetrabutylammonium Hydrogen Sulfate in HPLC?
While Tetrabutylammonium Hydrogen Sulfate is a commonly used reagent in HPLC analyses, there are some important considerations to keep in mind when working with this compound. For example, TBAHS can interact with some stationary phases and columns, so it is important to optimize the mobile phase composition and conditions to avoid any adverse effects on chromatographic performance. Additionally, TBAHS is a toxic compound and should be handled with care, using appropriate safety equipment and procedures to minimize exposure.
What are some potential applications or areas of research where Tetrabutylammonium Hydrogen Sulfate can be beneficial in HPLC?
Tetrabutylammonium Hydrogen Sulfate has a wide range of applications in HPLC analyses, particularly in the separation of charged or highly polar compounds. Some potential areas of research where TBAHS can be beneficial include pharmaceutical analysis, environmental monitoring, food and beverage testing, and bioanalytical studies. In each of these areas, TBAHS can help to improve the separation and identification of analytes, leading to more accurate and reliable results.
Tetrabutylammonium Hydrogen Sulfate (TBAHS) is a salt compound that is commonly used as an ion pairing reagent in High-Performance Liquid Chromatography (HPLC) analyses. It is typically used in reverse-phase chromatography to improve the resolution and retention of charged, water-soluble compounds by forming ion pairs with them. This allows for better separation and identification of analytes in complex sample matrices.
What are the benefits of using Tetrabutylammonium Hydrogen Sulfate in HPLC?
One of the main benefits of using Tetrabutylammonium Hydrogen Sulfate in HPLC is its ability to enhance the separation of charged compounds, particularly those that are highly polar or hydrophilic. By forming ion pairs with these compounds, TBAHS helps to improve their retention on the column and reduce their interactions with the stationary phase, resulting in sharper peaks and better resolution. Additionally, TBAHS can also improve the sensitivity and reproducibility of HPLC analyses, making it an invaluable tool for researchers and analysts working with challenging samples.
How is Tetrabutylammonium Hydrogen Sulfate typically added to HPLC mobile phases?
Tetrabutylammonium Hydrogen Sulfate is usually added to the mobile phase in HPLC as a solution in an organic solvent such as methanol or acetonitrile. The concentration of TBAHS in the mobile phase can vary depending on the specific application and the analytes being separated, but is typically in the range of 0.1-10 mM. It is important to ensure that the TBAHS solution is properly mixed with the mobile phase before use to ensure uniform distribution throughout the system.
Are there any considerations or precautions to keep in mind when using Tetrabutylammonium Hydrogen Sulfate in HPLC?
While Tetrabutylammonium Hydrogen Sulfate is a commonly used reagent in HPLC analyses, there are some important considerations to keep in mind when working with this compound. For example, TBAHS can interact with some stationary phases and columns, so it is important to optimize the mobile phase composition and conditions to avoid any adverse effects on chromatographic performance. Additionally, TBAHS is a toxic compound and should be handled with care, using appropriate safety equipment and procedures to minimize exposure.
What are some potential applications or areas of research where Tetrabutylammonium Hydrogen Sulfate can be beneficial in HPLC?
Tetrabutylammonium Hydrogen Sulfate has a wide range of applications in HPLC analyses, particularly in the separation of charged or highly polar compounds. Some potential areas of research where TBAHS can be beneficial include pharmaceutical analysis, environmental monitoring, food and beverage testing, and bioanalytical studies. In each of these areas, TBAHS can help to improve the separation and identification of analytes, leading to more accurate and reliable results.
