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| Product Name | Tetrabutylammonium di-Hydrogen Phosphate for HPLC |
| Chemical Formula | C16H36N O4P |
| CAS Number | 31441-58-0 |
| Molecular Weight | 345.43 g/mol |
| Appearance | White powder |
| Purity | ≥98% |
| Solubility | Soluble in water |
| Storage Conditions | Store at room temperature |
| Applications | Used as an ion-pairing reagent for HPLC analysis |
| Shelf Life | 2 years |
| Handling Precautions | Avoid inhalation or ingestion, wear protective gloves and clothing |
FAQ
What is Tetrabutylammonium di-Hydrogen Phosphate and its relevance in
HPLC?
Tetrabutylammonium di-Hydrogen Phosphate is a commonly used ion pairing reagent in reverse phase high-performance liquid chromatography (HPLC). It is used to enhance the separation of ionic compounds that are not retained or poorly retained by traditional reversed-phase columns due to their hydrophilicity or ionic nature.
How does Tetrabutylammonium di-Hydrogen Phosphate improve HPLC separations?
Tetrabutylammonium di-Hydrogen Phosphate acts as an ion pairing agent by forming electrostatic interactions with the charged functional groups of the analytes, making them more hydrophobic and increasing their retention on the reversed-phase column. This leads to improved peak shape, resolution, and sensitivity of the analytes in HPLC separations.
What are the advantages of using Tetrabutylammonium di-Hydrogen Phosphate in HPLC?
Some of the key advantages of using Tetrabutylammonium di-Hydrogen Phosphate in HPLC include:
- Improved retention and separation of ionic compounds
- Enhanced peak shape and resolution
- Increased sensitivity and accuracy of analyses
- Compatibility with a wide range of column chemistries and detection techniques
- Cost-effective solution for challenging separations
How should Tetrabutylammonium di-Hydrogen Phosphate be used in HPLC applications?
Tetrabutylammonium di-Hydrogen Phosphate is typically added to the mobile phase in concentrations ranging from 0.5 to 10 mM, depending on the specific analytes and separation conditions. It is important to optimize the concentration of the ion pairing reagent, as higher concentrations may lead to peak broadening and reduced sensitivity. Tetrabutylammonium di-Hydrogen Phosphate should be dissolved in the mobile phase solvent and filtered through a 0.2 µm membrane filter before use to prevent column blockage and assure the quality of the analysis.
Are there any specific considerations or precautions to keep in mind when using Tetrabutylammonium di-Hydrogen Phosphate in HPLC?
While Tetrabutylammonium di-Hydrogen Phosphate offers significant benefits in HPLC separations, there are some precautions to be aware of when using this ion pairing reagent:
- Tetrabutylammonium di-Hydrogen Phosphate is hygroscopic and should be stored in a cool, dry place to prevent moisture absorption and degradation.
- Care should be taken to avoid contamination of the mobile phase or column with particulate matter, as this can lead to blockages and chromatographic issues.
- The pH of the mobile phase should be monitored and adjusted as needed to ensure the stability and performance of the ion pairing reagent.
- Proper waste disposal procedures should be followed when discarding mobile phase solutions containing Tetrabutylammonium di-Hydrogen Phosphate to prevent environmental contamination.
Tetrabutylammonium di-Hydrogen Phosphate is a commonly used ion pairing reagent in reverse phase high-performance liquid chromatography (HPLC). It is used to enhance the separation of ionic compounds that are not retained or poorly retained by traditional reversed-phase columns due to their hydrophilicity or ionic nature.
How does Tetrabutylammonium di-Hydrogen Phosphate improve HPLC separations?
Tetrabutylammonium di-Hydrogen Phosphate acts as an ion pairing agent by forming electrostatic interactions with the charged functional groups of the analytes, making them more hydrophobic and increasing their retention on the reversed-phase column. This leads to improved peak shape, resolution, and sensitivity of the analytes in HPLC separations.
What are the advantages of using Tetrabutylammonium di-Hydrogen Phosphate in HPLC?
Some of the key advantages of using Tetrabutylammonium di-Hydrogen Phosphate in HPLC include:
- Improved retention and separation of ionic compounds
- Enhanced peak shape and resolution
- Increased sensitivity and accuracy of analyses
- Compatibility with a wide range of column chemistries and detection techniques
- Cost-effective solution for challenging separations
How should Tetrabutylammonium di-Hydrogen Phosphate be used in HPLC applications?
Tetrabutylammonium di-Hydrogen Phosphate is typically added to the mobile phase in concentrations ranging from 0.5 to 10 mM, depending on the specific analytes and separation conditions. It is important to optimize the concentration of the ion pairing reagent, as higher concentrations may lead to peak broadening and reduced sensitivity. Tetrabutylammonium di-Hydrogen Phosphate should be dissolved in the mobile phase solvent and filtered through a 0.2 µm membrane filter before use to prevent column blockage and assure the quality of the analysis.
Are there any specific considerations or precautions to keep in mind when using Tetrabutylammonium di-Hydrogen Phosphate in HPLC?
While Tetrabutylammonium di-Hydrogen Phosphate offers significant benefits in HPLC separations, there are some precautions to be aware of when using this ion pairing reagent:
- Tetrabutylammonium di-Hydrogen Phosphate is hygroscopic and should be stored in a cool, dry place to prevent moisture absorption and degradation.
- Care should be taken to avoid contamination of the mobile phase or column with particulate matter, as this can lead to blockages and chromatographic issues.
- The pH of the mobile phase should be monitored and adjusted as needed to ensure the stability and performance of the ion pairing reagent.
- Proper waste disposal procedures should be followed when discarding mobile phase solutions containing Tetrabutylammonium di-Hydrogen Phosphate to prevent environmental contamination.
