Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica

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Name Tris-(2-Carboxyethyl)-Phosphine Hydrochloride
Synonyms N/A
Molecular Formula C6H12ClO6P2
Molecular Weight 286.536 g/mol
CAS Number 51805-45-9
Appearance White to off-white powder
Solubility Soluble in water, ethanol, and methanol
Storage Conditions Store at room temperature, away from light
Purity ≥98%
Usage Reducing agent in protein biochemistry
Applications Used in protein folding and disulfide bond reduction
Handling Avoid inhalation, ingestion, and skin contact. Use in a well-ventilated area
Shelf Life 2 years when stored properly
FAQ
What is Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica and how is it used in biochemistry studies?

Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica is a potent reducing agent used in various biochemical applications. It is commonly used to reduce disulfide bonds in proteins and peptides, making it an essential tool in protein research and analysis. This compound is particularly effective in maintaining the reduced state of proteins during purification, allowing researchers to study the structure and function of proteins in their native state.

What are the advantages of using Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica in biochemical studies?

One of the main advantages of using Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica is its high stability and reactivity as a reducing agent. Unlike other reducing agents, Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica is highly soluble in water and remains active under a wide range of pH conditions. This makes it ideal for use in complex biological systems where maintaining protein stability is crucial.

How does Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica compare to other reducing agents commonly used in biochemistry?

Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica offers several advantages over other reducing agents commonly used in biochemistry. Firstly, it is odorless and colorless, making it easy to handle and work with in the laboratory. Additionally, Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica is highly stable and does not react with oxygen or other biological molecules, ensuring accurate and reliable results in experiments.

What are some common applications of Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica in biochemistry research?

Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica is widely used in biochemistry research for various applications, including protein purification, protein analysis, and structural biology studies. It is particularly useful in reducing disulfide bonds in proteins, enabling researchers to study the native structure and function of proteins. Additionally, Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica is commonly used in Western blotting, mass spectrometry, and other analytical techniques to analyze protein samples.

What precautions should be taken when working with Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica in the laboratory?

When working with Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica in the laboratory, it is important to follow standard safety precautions for handling chemicals. This includes wearing appropriate personal protective equipment, such as gloves and goggles, and working in a well-ventilated area to avoid inhalation of fumes. Additionally, Tris-(2-Carboxyethyl)-Phosphine Hydrochloride BioChemica should be stored in a cool, dry place away from heat and moisture to prevent degradation.
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