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| Name | Tris Hydrochloride |
| Product Type | Buffer solution |
| Chemical Formula | C4H11NO3.HCl |
| Molecular Weight | 157.60 g/mol |
| Appearance | White crystalline powder |
| Solubility | Soluble in water |
| PH Range | 7.0-9.0 |
| Storage Conditions | Store in a cool, dry place |
| Shelf Life | 2 years |
| Applications | Used as a buffer in biochemistry and molecular biology |
| Purity | Minimum 99.0% |
| Cas Number | 1185-53-1 |
FAQ
What is Tris Hydrochloride and what are its uses in buffer solutions?
Tris Hydrochloride is a commonly used buffer component in biochemical and molecular biology research. It helps maintain a stable pH in solutions, making it ideal for various laboratory applications such as protein purification, enzyme assays, and DNA electrophoresis.
How does Tris Hydrochloride work as a buffer solution?
Tris Hydrochloride acts as a buffer solution by accepting or donating protons to maintain a constant pH in a solution. It has a pKa value of around 8.1, which allows it to effectively buffer solutions in the physiological pH range of 7.2 to 9.2.
What are the advantages of using Tris Hydrochloride over other buffer components?
One of the main advantages of using Tris Hydrochloride as a buffer component is its ability to maintain a stable pH over a broad range of temperatures. It is also relatively inexpensive and easy to prepare, making it a popular choice for many researchers.
Can Tris Hydrochloride be used in combination with other buffer components?
Yes, Tris Hydrochloride can be used in combination with other buffer components to create custom buffer systems tailored to specific experimental needs. It is often used in combination with other buffers such as HEPES or phosphate buffers to increase buffering capacity or adjust pH.
Are there any precautions to consider when using Tris Hydrochloride in buffer solutions?
While Tris Hydrochloride is generally safe to use in laboratory settings, it is important to handle it with caution as it can be irritating to the skin, eyes, and respiratory tract. It is recommended to wear appropriate personal protective equipment when working with Tris Hydrochloride and to follow proper handling and disposal procedures.
Tris Hydrochloride is a commonly used buffer component in biochemical and molecular biology research. It helps maintain a stable pH in solutions, making it ideal for various laboratory applications such as protein purification, enzyme assays, and DNA electrophoresis.
How does Tris Hydrochloride work as a buffer solution?
Tris Hydrochloride acts as a buffer solution by accepting or donating protons to maintain a constant pH in a solution. It has a pKa value of around 8.1, which allows it to effectively buffer solutions in the physiological pH range of 7.2 to 9.2.
What are the advantages of using Tris Hydrochloride over other buffer components?
One of the main advantages of using Tris Hydrochloride as a buffer component is its ability to maintain a stable pH over a broad range of temperatures. It is also relatively inexpensive and easy to prepare, making it a popular choice for many researchers.
Can Tris Hydrochloride be used in combination with other buffer components?
Yes, Tris Hydrochloride can be used in combination with other buffer components to create custom buffer systems tailored to specific experimental needs. It is often used in combination with other buffers such as HEPES or phosphate buffers to increase buffering capacity or adjust pH.
Are there any precautions to consider when using Tris Hydrochloride in buffer solutions?
While Tris Hydrochloride is generally safe to use in laboratory settings, it is important to handle it with caution as it can be irritating to the skin, eyes, and respiratory tract. It is recommended to wear appropriate personal protective equipment when working with Tris Hydrochloride and to follow proper handling and disposal procedures.
