Back
| Name | Tris Hydrochloride for molecular biology |
| Product Type | Buffer |
| Usage | Common buffer for molecular biology applications |
| Purity | ≥ 99% |
| Form | Powder |
| Solubility | Soluble in water |
| Storage Conditions | Store at room temperature |
| PH | 7.0-9.0 |
| Molecular Weight | 157.60 g/mol |
| CAS Number | 1185-53-1 |
| EC Number | 214-227-5 |
FAQ
What is Tris Hydrochloride and what is its role in molecular biology?
Tris Hydrochloride is a buffer solution commonly used in molecular biology applications. It helps maintain a stable pH in the reaction environment, which is crucial for the functionality of enzymes and other molecules used in experiments. Tris Hydrochloride is also known for its high solubility in water, making it easy to prepare solutions with precise concentrations.
How is Tris Hydrochloride prepared and what are the recommended storage conditions?
Tris Hydrochloride can be prepared by dissolving the powder in water to the desired concentration. It is important to mix the solution thoroughly to ensure uniformity. Once prepared, Tris Hydrochloride should be stored at room temperature in a dry place away from direct sunlight. It is also recommended to store the solution in a tightly sealed container to prevent contamination.
What are the key advantages of using Tris Hydrochloride in molecular biology experiments?
One of the main advantages of using Tris Hydrochloride is its ability to maintain a stable pH level, which is crucial for the success of many molecular biology techniques. Additionally, Tris Hydrochloride is compatible with a wide range of biological molecules and enzymes, making it a versatile choice for different applications. Its high solubility and ease of preparation also make it a convenient option for researchers.
Are there any specific precautions to be taken while working with Tris Hydrochloride?
While Tris Hydrochloride is generally considered safe to use, there are a few precautions that researchers should keep in mind. Avoid inhaling the powder or solution, as it may cause irritation to the respiratory system. It is also important to wear appropriate personal protective equipment, such as gloves and goggles, when handling Tris Hydrochloride. In case of accidental contact with the skin or eyes, rinse thoroughly with water and seek medical attention if necessary.
How can Tris Hydrochloride be used in specific molecular biology techniques such as PCR or gel electrophoresis?
Tris Hydrochloride can be used as a buffer solution in various molecular biology techniques, including PCR and gel electrophoresis. In PCR, Tris Hydrochloride helps maintain the optimal pH for the activity of the DNA polymerase enzyme, which is essential for amplifying DNA sequences. In gel electrophoresis, Tris Hydrochloride is used as a buffer in both the running buffer and the gel itself, ensuring the proper migration of DNA molecules through the gel.
What are the recommended concentrations of Tris Hydrochloride for different molecular biology applications?
The optimal concentration of Tris Hydrochloride may vary depending on the specific application. For general use as a buffer solution, a concentration of 10-50 mM is commonly recommended. However, researchers should refer to literature references or consult with colleagues for specific protocols that may require different concentrations of Tris Hydrochloride. It is important to accurately prepare the solution to ensure reliable results in experiments.
Overall, Tris Hydrochloride is a versatile and reliable buffer solution that plays a crucial role in maintaining pH stability in molecular biology experiments. Its compatibility with a wide range of biological molecules and enzymes, along with its ease of preparation and storage, make it a popular choice among researchers. By following recommended guidelines for handling and using Tris Hydrochloride, scientists can achieve consistent and reproducible results in their experiments.
Tris Hydrochloride is a buffer solution commonly used in molecular biology applications. It helps maintain a stable pH in the reaction environment, which is crucial for the functionality of enzymes and other molecules used in experiments. Tris Hydrochloride is also known for its high solubility in water, making it easy to prepare solutions with precise concentrations.
How is Tris Hydrochloride prepared and what are the recommended storage conditions?
Tris Hydrochloride can be prepared by dissolving the powder in water to the desired concentration. It is important to mix the solution thoroughly to ensure uniformity. Once prepared, Tris Hydrochloride should be stored at room temperature in a dry place away from direct sunlight. It is also recommended to store the solution in a tightly sealed container to prevent contamination.
What are the key advantages of using Tris Hydrochloride in molecular biology experiments?
One of the main advantages of using Tris Hydrochloride is its ability to maintain a stable pH level, which is crucial for the success of many molecular biology techniques. Additionally, Tris Hydrochloride is compatible with a wide range of biological molecules and enzymes, making it a versatile choice for different applications. Its high solubility and ease of preparation also make it a convenient option for researchers.
Are there any specific precautions to be taken while working with Tris Hydrochloride?
While Tris Hydrochloride is generally considered safe to use, there are a few precautions that researchers should keep in mind. Avoid inhaling the powder or solution, as it may cause irritation to the respiratory system. It is also important to wear appropriate personal protective equipment, such as gloves and goggles, when handling Tris Hydrochloride. In case of accidental contact with the skin or eyes, rinse thoroughly with water and seek medical attention if necessary.
How can Tris Hydrochloride be used in specific molecular biology techniques such as PCR or gel electrophoresis?
Tris Hydrochloride can be used as a buffer solution in various molecular biology techniques, including PCR and gel electrophoresis. In PCR, Tris Hydrochloride helps maintain the optimal pH for the activity of the DNA polymerase enzyme, which is essential for amplifying DNA sequences. In gel electrophoresis, Tris Hydrochloride is used as a buffer in both the running buffer and the gel itself, ensuring the proper migration of DNA molecules through the gel.
What are the recommended concentrations of Tris Hydrochloride for different molecular biology applications?
The optimal concentration of Tris Hydrochloride may vary depending on the specific application. For general use as a buffer solution, a concentration of 10-50 mM is commonly recommended. However, researchers should refer to literature references or consult with colleagues for specific protocols that may require different concentrations of Tris Hydrochloride. It is important to accurately prepare the solution to ensure reliable results in experiments.
Overall, Tris Hydrochloride is a versatile and reliable buffer solution that plays a crucial role in maintaining pH stability in molecular biology experiments. Its compatibility with a wide range of biological molecules and enzymes, along with its ease of preparation and storage, make it a popular choice among researchers. By following recommended guidelines for handling and using Tris Hydrochloride, scientists can achieve consistent and reproducible results in their experiments.
