X-Glucuro CHA Salt BioChemica

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Product Name X-Glucuro CHA Salt BioChemica
CAS Number 114263-16-3
Molecular Formula C21H28Cl2N2O7
Molecular Weight 504.36 g/mol
Appearance White to off-white solid
Solubility Soluble in water
Storage Conditions Store at room temperature
Purity Minimum 95%
Application Biochemical research
Synonyms 5-Bromo-4-chloro-3-indoxyl-alpha-D-glucuronide CHA salt
Handling Precautions Avoid contact with skin and eyes
FAQ
What is X-Glucuro CHA Salt BioChemica and what are its applications?
X-Glucuro CHA Salt BioChemica is a chemical compound that is commonly used in biochemical research to detect the activity of β-glucuronidase enzymes. It is frequently utilized as a substrate in colorimetric assays to measure β-glucuronidase activity in various samples. This compound has a wide range of applications in molecular biology, biochemistry, and enzymology research.

How does X-Glucuro CHA Salt BioChemica work in detecting β-glucuronidase activity?
X-Glucuro CHA Salt BioChemica is a chromogenic substrate that can be cleaved by β-glucuronidase, an enzyme commonly found in bacteria and some eukaryotic cells. When β-glucuronidase catalyzes the hydrolysis of X-Glucuro CHA Salt BioChemica, it releases a blue dye that can be easily detected and quantified using spectrophotometry. This colorimetric method allows researchers to measure the presence and activity of β-glucuronidase in biological samples.

What are the advantages of using X-Glucuro CHA Salt BioChemica in β-glucuronidase assays?
X-Glucuro CHA Salt BioChemica offers several advantages when used in β-glucuronidase assays. Firstly, its colorimetric detection method provides a simple and efficient way to measure enzyme activity in a wide range of samples. Additionally, this compound is stable and can be stored for extended periods, making it a convenient choice for biochemical research. Moreover, X-Glucuro CHA Salt BioChemica is highly specific to β-glucuronidase, ensuring accurate and reliable results in enzymatic assays.

Can X-Glucuro CHA Salt BioChemica be used in combination with other substrates or reagents?
Yes, X-Glucuro CHA Salt BioChemica can be used in combination with other substrates or reagents to enhance the detection of β-glucuronidase activity. Researchers often employ X-Glucuro CHA Salt BioChemica alongside other colorimetric substrates to conduct multiplex assays and measure the activity of multiple enzymes simultaneously. Additionally, this compound can be paired with inhibitors or activators of β-glucuronidase to study the regulation of enzyme activity in different biological contexts.

What are the recommended storage and handling procedures for X-Glucuro CHA Salt BioChemica?
X-Glucuro CHA Salt BioChemica should be stored in a cool, dry place away from light and moisture to maintain its stability and integrity. It is essential to handle this compound with care and follow proper safety precautions when working with biochemical reagents. Prior to use, researchers should ensure that X-Glucuro CHA Salt BioChemica is dissolved in the appropriate solvent and stored at the recommended temperature to prevent degradation or contamination.
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