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| Product Name | 2-Propanol for HPLC |
| Supplier | Sigma-Aldrich |
| Purity | ≥ 99.9% |
| Grade | HPLC grade |
| Molecular Weight | 60.10 g/mol |
| Appearance | Clear colorless liquid |
| Boiling Point | 82.6 °C |
| Melting Point | -88.5 °C |
| Density | 0.785 g/mL at 25 °C |
| Solubility | Miscible with water, ethanol, ether |
| Storage Condition | Store at room temperature |
| Cas Number | 67-63-0 |
FAQ
What is 2-Propanol and how is it used in HPLC?
2-Propanol, also known as isopropanol or IPA, is a common solvent used in various analytical techniques, including high-performance liquid chromatography (HPLC). In HPLC, 2-Propanol is typically used as a mobile phase solvent due to its ability to dissolve a wide range of compounds, making it suitable for separating and analyzing complex mixtures.
Why is 2-Propanol a preferred solvent for HPLC applications?
2-Propanol is a popular choice for HPLC applications due to its favorable characteristics as a solvent. It is miscible in water and many organic solvents, which allows for excellent chromatographic performance and effective separation of analytes. Additionally, 2-Propanol has a low UV cut-off, making it suitable for UV detection in HPLC systems. Its volatility, low viscosity, and low UV absorption make it an ideal choice for a wide range of HPLC separations.
What are the advantages of using 2-Propanol in HPLC over other solvents?
There are several advantages to using 2-Propanol in HPLC over other solvents. Firstly, 2-Propanol has a lower UV cut-off compared to other common solvents like methanol or acetonitrile, allowing for more sensitive UV detection in HPLC analyses. Additionally, 2-Propanol is less toxic than some other solvents, making it a safer option for laboratory personnel. Its compatibility with a wide range of analytes and ability to provide effective separations make it a versatile choice for HPLC applications.
How can 2-Propanol be optimized for HPLC methods?
Optimizing 2-Propanol for HPLC methods involves several considerations to ensure optimal performance. The purity and quality of the solvent are crucial, as impurities can negatively impact chromatographic results. Additionally, the selection of the appropriate column, mobile phase composition, and gradient conditions can all affect the performance of 2-Propanol in HPLC analyses. Regular maintenance and calibration of the HPLC system are also essential to ensure reliable results when using 2-Propanol as a solvent.
In conclusion, 2-Propanol is a versatile and effective solvent for HPLC applications, offering several advantages over other solvents. By understanding its properties and optimizing its use in HPLC methods, researchers can achieve accurate and reliable results in their analyses.
2-Propanol, also known as isopropanol or IPA, is a common solvent used in various analytical techniques, including high-performance liquid chromatography (HPLC). In HPLC, 2-Propanol is typically used as a mobile phase solvent due to its ability to dissolve a wide range of compounds, making it suitable for separating and analyzing complex mixtures.
Why is 2-Propanol a preferred solvent for HPLC applications?
2-Propanol is a popular choice for HPLC applications due to its favorable characteristics as a solvent. It is miscible in water and many organic solvents, which allows for excellent chromatographic performance and effective separation of analytes. Additionally, 2-Propanol has a low UV cut-off, making it suitable for UV detection in HPLC systems. Its volatility, low viscosity, and low UV absorption make it an ideal choice for a wide range of HPLC separations.
What are the advantages of using 2-Propanol in HPLC over other solvents?
There are several advantages to using 2-Propanol in HPLC over other solvents. Firstly, 2-Propanol has a lower UV cut-off compared to other common solvents like methanol or acetonitrile, allowing for more sensitive UV detection in HPLC analyses. Additionally, 2-Propanol is less toxic than some other solvents, making it a safer option for laboratory personnel. Its compatibility with a wide range of analytes and ability to provide effective separations make it a versatile choice for HPLC applications.
How can 2-Propanol be optimized for HPLC methods?
Optimizing 2-Propanol for HPLC methods involves several considerations to ensure optimal performance. The purity and quality of the solvent are crucial, as impurities can negatively impact chromatographic results. Additionally, the selection of the appropriate column, mobile phase composition, and gradient conditions can all affect the performance of 2-Propanol in HPLC analyses. Regular maintenance and calibration of the HPLC system are also essential to ensure reliable results when using 2-Propanol as a solvent.
In conclusion, 2-Propanol is a versatile and effective solvent for HPLC applications, offering several advantages over other solvents. By understanding its properties and optimizing its use in HPLC methods, researchers can achieve accurate and reliable results in their analyses.
