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| Name | 2-Propanol |
| Category | Molecular Biology |
| Formula | C3H8O |
| Molar Mass | 60.10 g/mol |
| Boiling Point | 82.6°C |
| Melting Point | -89.5°C |
| Density | 0.786 g/cm³ |
| Solubility | Miscible in water |
| Purity | ≥ 99.5% |
| Storage Condition | Store at room temperature |
| Usage | Commonly used as a solvent in molecular biology |
| Appearance | Colorless liquid |
| Odor | Characteristic alcohol-like odor |
| Flash Point | 11.7°C |
| Autoignition Temp | 399°C |
| Risk Phrases | Highly flammable, Causes serious eye irritation |
| Safety Phrases | Keep away from heat/sparks/open flames/hot surfaces. - No smoking. Wear protective gloves/eye protection |
FAQ
What is 2-Propanol and how is it used in molecular biology?
2-Propanol, also known as isopropanol or isopropyl alcohol, is a common solvent that is widely used in molecular biology laboratories for various applications. It is commonly used for DNA and RNA precipitation, as well as for general cleaning and sterilization purposes.
How is 2-Propanol different from other solvents used in molecular biology?
2-Propanol is preferred over other solvents such as ethanol or methanol in molecular biology applications due to its lower toxicity and volatility. It is less flammable compared to ethanol, making it a safer option for laboratory use. Additionally, 2-Propanol is less likely to denature proteins or nucleic acids during precipitation procedures, making it a more reliable solvent for molecular biology experiments.
What are the benefits of using 2-Propanol in DNA and RNA precipitation?
2-Propanol is commonly used in DNA and RNA precipitation steps in molecular biology applications because of its ability to efficiently precipitate nucleic acids while leaving behind impurities such as salts and proteins. It is an essential reagent for purifying nucleic acids for downstream applications such as PCR, sequencing, and cloning.
How should 2-Propanol be stored and handled in the laboratory?
2-Propanol should be stored in a cool, dry place away from heat sources and direct sunlight. It should be handled with care, as it is flammable and may irritate the skin and eyes upon contact. It is important to wear appropriate personal protective equipment, such as gloves and safety goggles, when working with 2-Propanol to minimize the risk of exposure.
What are some common troubleshooting tips when using 2-Propanol in molecular biology experiments?
If you are experiencing issues with DNA or RNA precipitation using 2-Propanol, there are a few troubleshooting tips to consider. First, make sure that the 2-Propanol is cold enough to promote nucleic acid precipitation - storing it in the refrigerator or freezer can help achieve this. Additionally, ensure that the nucleic acid solution is properly mixed with the 2-Propanol to facilitate precipitation. If you are still encountering problems, consider adjusting the pH of the solution or increasing the concentration of salts to optimize precipitation efficiency.
2-Propanol, also known as isopropanol or isopropyl alcohol, is a common solvent that is widely used in molecular biology laboratories for various applications. It is commonly used for DNA and RNA precipitation, as well as for general cleaning and sterilization purposes.
How is 2-Propanol different from other solvents used in molecular biology?
2-Propanol is preferred over other solvents such as ethanol or methanol in molecular biology applications due to its lower toxicity and volatility. It is less flammable compared to ethanol, making it a safer option for laboratory use. Additionally, 2-Propanol is less likely to denature proteins or nucleic acids during precipitation procedures, making it a more reliable solvent for molecular biology experiments.
What are the benefits of using 2-Propanol in DNA and RNA precipitation?
2-Propanol is commonly used in DNA and RNA precipitation steps in molecular biology applications because of its ability to efficiently precipitate nucleic acids while leaving behind impurities such as salts and proteins. It is an essential reagent for purifying nucleic acids for downstream applications such as PCR, sequencing, and cloning.
How should 2-Propanol be stored and handled in the laboratory?
2-Propanol should be stored in a cool, dry place away from heat sources and direct sunlight. It should be handled with care, as it is flammable and may irritate the skin and eyes upon contact. It is important to wear appropriate personal protective equipment, such as gloves and safety goggles, when working with 2-Propanol to minimize the risk of exposure.
What are some common troubleshooting tips when using 2-Propanol in molecular biology experiments?
If you are experiencing issues with DNA or RNA precipitation using 2-Propanol, there are a few troubleshooting tips to consider. First, make sure that the 2-Propanol is cold enough to promote nucleic acid precipitation - storing it in the refrigerator or freezer can help achieve this. Additionally, ensure that the nucleic acid solution is properly mixed with the 2-Propanol to facilitate precipitation. If you are still encountering problems, consider adjusting the pH of the solution or increasing the concentration of salts to optimize precipitation efficiency.
