Bouin Liquor for clinical diagnosis

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Name Bouin Liquor for Clinical Diagnosis
Manufacturer Sigma-Aldrich
Volume 500 mL
Composition Picric acid, formalin, acetic acid
Purpose Fixation solution for histology and cytology samples
Stability Store at room temperature, protect from light
Color Yellow
Odor Pungent
PH 2.7
Shelf Life 2 years
Storage Conditions Store tightly capped in a well-ventilated area
FAQ
What is Bouin Liquor and how is it used in clinical diagnosis?

Bouin Liquor is a solution commonly used in histology laboratories for preserving tissue samples for clinical diagnosis. It is a mixture of formaldehyde, acetic acid, and picric acid that fixes tissue samples by cross-linking proteins and nucleic acids, preventing their degradation.

How does Bouin Liquor differ from other tissue fixatives?

Bouin Liquor is known for its ability to preserve tissue morphology and antigenicity better than other fixatives such as formalin or alcohol-based fixatives. This makes it a preferred choice for certain types of tissue samples that require excellent preservation of cellular structures.

What are the advantages of using Bouin Liquor for clinical diagnosis?

One of the main advantages of using Bouin Liquor is its ability to provide superior preservation of tissue morphology and antigenicity, which is crucial for accurate diagnosis in pathology. Additionally, Bouin Liquor is cost-effective and easy to prepare, making it a practical choice for laboratories.

Are there any limitations or precautions to consider when using Bouin Liquor?

While Bouin Liquor is an effective fixative, it is important to note that it contains toxic ingredients such as picric acid. Therefore, proper safety measures should be taken when handling and disposing of Bouin Liquor to prevent exposure. Additionally, Bouin-fixed samples may not be suitable for certain downstream applications such as immunohistochemistry.

In what clinical scenarios is Bouin Liquor particularly beneficial?

Bouin Liquor is particularly beneficial for preserving tissues that require excellent structural preservation, such as gastrointestinal biopsies or endocrine organs. Its superior antigenicity preservation also makes it a preferred fixative for certain immunohistochemistry studies where accurate antigen detection is crucial.
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