Dimethyl Sulfoxide for cell culture

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Product Name Dimethyl Sulfoxide for cell culture
0 Chemical Formula: C2H6OS
1 Synonyms: DMSO
2 CAS Number: 67-68-5
3 Molecular Weight: 78.13 g/mol
4 Appearance: Clear, colorless liquid
5 Purity: ≥99.9%
6 Solubility: Soluble in water and most organic solvents
7 Storage: Store in a cool, dry place
8 Usage: Cryoprotectant for cell culture
9 Applications: Cell cryopreservation, drug delivery, and as a solvent
FAQ
What is Dimethyl Sulfoxide (DMSO) and how is it used in cell culture?

Dimethyl Sulfoxide, commonly known as DMSO, is a highly versatile solvent used in cell culture applications. It is widely utilized to dissolve various compounds and chemicals that are used in cell culture experiments. DMSO also has the ability to penetrate cell membranes, making it an effective carrier of other substances into cells. This property makes DMSO an invaluable tool in molecular biology and biotechnology research.

What are the benefits of using Dimethyl Sulfoxide in cell culture?

There are several benefits to using DMSO in cell culture. Firstly, DMSO is known for its ability to enhance cell membrane permeability, allowing for the efficient delivery of various compounds into cells. This property is particularly useful in experiments involving the introduction of nucleic acids or proteins into cells. Additionally, DMSO has antioxidant properties that can help protect cells from oxidative stress. It is also a cryoprotectant, meaning that it can be used to preserve cells at low temperatures without causing damage.

How should Dimethyl Sulfoxide be stored and handled in the laboratory?

When working with Dimethyl Sulfoxide in the laboratory, it is important to store it properly to maintain its stability and efficacy. DMSO should be kept in a tightly sealed container at room temperature, away from direct sunlight and moisture. It is advisable to wear appropriate personal protective equipment, such as gloves and goggles, when handling DMSO to prevent skin and eye irritation. Additionally, DMSO should be used in a well-ventilated area to prevent inhalation of fumes.

Are there any potential risks or side effects associated with using Dimethyl Sulfoxide in cell culture?

While Dimethyl Sulfoxide is widely used in cell culture and biotechnology research, there are some potential risks and side effects associated with its use. DMSO is known to have a strong odor that can be unpleasant to some individuals. It may also cause skin irritation or allergic reactions in some people. In addition, prolonged or repeated exposure to DMSO can lead to dryness and cracking of the skin. It is important to follow proper safety protocols when working with DMSO to minimize these risks.

How can Dimethyl Sulfoxide be used effectively in cell culture experiments?

Dimethyl Sulfoxide can be used in a variety of ways in cell culture experiments to enhance the efficiency and success of the study. It can be used as a solvent to dissolve compounds that are insoluble in water or other solvents. DMSO can also be used as a cryoprotectant to preserve cells for long-term storage. Additionally, DMSO can be added to cell culture media to improve cell viability and growth. Overall, incorporating DMSO into cell culture experiments can help researchers achieve more reliable and reproducible results.
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