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| Product Name | Formamide deionized for molecular biology |
| Product Type | Chemical reagent |
| Purity | Deionized |
| Application | Molecular biology |
| Storage Condition | Store at room temperature |
| Appearance | Clear colorless liquid |
| Molecular Formula | CH3NO |
| Molecular Weight | 45.04 g/mol |
| Boiling Point | 210-212°C |
| Melting Point | -2 to 2 °C |
| Flash Point | 83 °C |
| Density | 1.133 g/mL |
| Solubility | Miscible with water, ethanol, acetone |
| Cas Number | 75-12-7 |
| Manufacturer | Various |
| Shelf Life | 2 years |
| Packaging Size | Available in various sizes |
FAQ
What is formamide deionized and how is it used in molecular biology
applications?
Formamide deionized is a purified form of formamide that is commonly used in molecular biology laboratories for various applications. It is often used in nucleic acid hybridization experiments, such as Southern and Northern blotting, as well as in situ hybridization techniques. Formamide deionized helps to denature DNA and RNA molecules, allowing for better binding of probes during hybridization experiments.
What are the advantages of using formamide deionized over regular formamide?
Formamide deionized is specially treated to remove impurities and contaminants that may interfere with molecular biology experiments. This ensures that the formamide is of high purity and quality, resulting in more reliable and consistent results. Additionally, formamide deionized is free of nuclease activity, which is important for preserving the integrity of nucleic acids during hybridization experiments.
How should formamide deionized be stored and handled in the laboratory?
Formamide deionized should be stored in a cool, dark place away from light and heat sources to prevent degradation. It is important to tightly seal the container after each use to avoid contamination. When handling formamide deionized, it is recommended to wear appropriate personal protective equipment, such as gloves and goggles, to prevent skin and eye irritation.
Can formamide deionized be used in combination with other reagents commonly used in molecular biology experiments?
Yes, formamide deionized can be used in conjunction with other reagents commonly used in molecular biology experiments, such as hybridization buffers, DNA probes, and blocking agents. It is important to optimize the concentration of formamide deionized in the hybridization buffer to achieve the desired denaturation and hybridization conditions for specific applications.
What are some tips for troubleshooting common issues encountered when using formamide deionized in molecular biology experiments?
If you encounter issues such as high background signal or weak signal intensity in your hybridization experiments, it may be helpful to adjust the formamide deionized concentration in the hybridization buffer or optimize the hybridization conditions. Additionally, it is important to ensure that all reagents used are of high quality and stored properly to prevent contamination. If problems persist, consulting with colleagues or referring to literature may provide solutions to troubleshoot the issue.
Formamide deionized is a purified form of formamide that is commonly used in molecular biology laboratories for various applications. It is often used in nucleic acid hybridization experiments, such as Southern and Northern blotting, as well as in situ hybridization techniques. Formamide deionized helps to denature DNA and RNA molecules, allowing for better binding of probes during hybridization experiments.
What are the advantages of using formamide deionized over regular formamide?
Formamide deionized is specially treated to remove impurities and contaminants that may interfere with molecular biology experiments. This ensures that the formamide is of high purity and quality, resulting in more reliable and consistent results. Additionally, formamide deionized is free of nuclease activity, which is important for preserving the integrity of nucleic acids during hybridization experiments.
How should formamide deionized be stored and handled in the laboratory?
Formamide deionized should be stored in a cool, dark place away from light and heat sources to prevent degradation. It is important to tightly seal the container after each use to avoid contamination. When handling formamide deionized, it is recommended to wear appropriate personal protective equipment, such as gloves and goggles, to prevent skin and eye irritation.
Can formamide deionized be used in combination with other reagents commonly used in molecular biology experiments?
Yes, formamide deionized can be used in conjunction with other reagents commonly used in molecular biology experiments, such as hybridization buffers, DNA probes, and blocking agents. It is important to optimize the concentration of formamide deionized in the hybridization buffer to achieve the desired denaturation and hybridization conditions for specific applications.
What are some tips for troubleshooting common issues encountered when using formamide deionized in molecular biology experiments?
If you encounter issues such as high background signal or weak signal intensity in your hybridization experiments, it may be helpful to adjust the formamide deionized concentration in the hybridization buffer or optimize the hybridization conditions. Additionally, it is important to ensure that all reagents used are of high quality and stored properly to prevent contamination. If problems persist, consulting with colleagues or referring to literature may provide solutions to troubleshoot the issue.
