Isooctane for UV, IR, HPLC, ACS

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Name Isooctane for UV, IR, HPLC, ACS
Formula C8H18
Molecular Weight 114.23 g/mol
Appearance Colorless liquid
Boiling Point 99.2 °C
Melting Point -107.4 °C
Density 0.692 g/mL
Solubility Insoluble in water
Purity ≥99.5%
Cas Number 540-84-1
Storage Condition Store in a cool, dry place
Applications Commonly used as a standard for octane rating in fuels
FAQ
What is Isooctane and how is it used for UV, IR, HPLC, and ACS applications?
Isooctane is a type of isomer of octane, primarily used as a reference standard in various analytical techniques such as UV spectroscopy, IR spectroscopy, HPLC (High-Performance Liquid Chromatography), and ACS (American Chemical Society) applications. It is commonly utilized as a non-polar solvent due to its chemical properties, making it suitable for a wide range of analytical methods.

How does Isooctane perform in UV spectroscopy?
Isooctane is often used in UV spectroscopy as a reference standard to calibrate and validate the instrument's performance. Its low absorbance in the UV range makes it an ideal blank solvent for measuring the absorbance of other compounds. By using Isooctane as a reference, researchers can accurately determine the absorption spectrum of samples and assess the purity of compounds.

In what way is Isooctane utilized in IR spectroscopy?
In IR spectroscopy, Isooctane serves as a reference standard to facilitate the identification of functional groups and chemical bonds in organic compounds. Its low absorption in the IR region allows for clear and accurate spectral measurements, making it an essential tool for chemists and researchers. By comparing the IR spectra of unknown samples to that of Isooctane, scientists can deduce valuable information about the molecular structure of the compounds under investigation.

How is Isooctane beneficial for HPLC applications?
In HPLC, Isooctane functions as a solvent for separating and analyzing a variety of compounds based on their interactions with the stationary phase. Its non-polar nature makes it suitable for resolving compounds with similar properties, enabling researchers to achieve high resolution and efficiency in their chromatographic separations. Additionally, Isooctane's compatibility with a wide range of detectors and columns makes it a versatile choice for HPLC analyses.

Why is Isooctane commonly used in ACS methods?
Isooctane is widely employed in ACS (American Chemical Society) methods due to its reliability and reproducibility in chemical analyses. As a well-established reference standard, Isooctane ensures the accuracy of measurements and the consistency of results across different laboratories. Its chemical stability and inertness make it a preferred choice for various ACS applications, guaranteeing the quality and integrity of experimental data.
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