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| Name | n-Hexane |
| Type | Solvent |
| Purity | ≥99% |
| Form | Liquid |
| Molecular Weight | 86.18 g/mol |
| Boiling Point | 68.7°C |
| Melting Point | -95°C |
| Density | 0.659 g/mL |
| Flash Point | -22°C |
| UV Absorbance | λmax: 210 nm |
| IR Spectra | Clear spectrum |
| HPLC Compatibility | Suitable |
| Solubility | Insoluble in water |
| Odor | Mild hydrocarbon odor |
| Storage Conditions | Keep away from heat, sparks, and open flames |
| CAS Number | 110-54-3 |
FAQ
What is n-Hexane and how is it used in UV, IR, and HPLC?
n-Hexane is a straight-chain alkane with the chemical formula C6H14. It is a common solvent used in various analytical techniques such as UV (ultraviolet), IR (infrared), and HPLC (high-performance liquid chromatography). In UV spectroscopy, n-Hexane is often used as a solvent for preparing samples or as a carrier solvent in mobile phases. In IR spectroscopy, n-Hexane can be used to dissolve nonpolar compounds for analysis. In HPLC, n-Hexane is utilized as a mobile phase solvent in reversed-phase chromatography.
How does n-Hexane perform in UV spectroscopy applications?
n-Hexane is commonly used in UV spectroscopy due to its UV-transparent nature in the range of 200-220 nm. This makes it ideal for analyzing compounds that absorb in this region. Additionally, n-Hexane has a low UV absorption which minimizes interference with the sample being analyzed. Its low viscosity and volatility make it a suitable solvent for UV spectroscopy applications.
What are the advantages of using n-Hexane in IR spectroscopy?
n-Hexane is a nonpolar solvent that is commonly used in IR spectroscopy for dissolving nonpolar compounds. Being nonpolar, n-Hexane interacts minimally with most organic compounds, ensuring that the IR spectra obtained are not distorted by solvent peaks. Its compatibility with a wide range of compounds makes it a versatile choice for IR spectroscopy applications. Additionally, n-Hexane is relatively easy to evaporate, leaving minimal residue on the sample surface.
How is n-Hexane used in HPLC applications?
In HPLC, n-Hexane is often used as a mobile phase solvent in reversed-phase chromatography. It is particularly useful for the separation of nonpolar compounds due to its low polarity. n-Hexane can be combined with other solvents such as methanol or acetonitrile to optimize the chromatographic separation of compounds with varying polarities. Its volatility and compatibility with common stationary phases make n-Hexane a popular choice for HPLC applications.
What precautions should be taken when handling n-Hexane in the laboratory?
n-Hexane is a highly flammable liquid that should be handled with care in the laboratory. It should be stored in a cool, well-ventilated area away from sources of ignition. When working with n-Hexane, proper personal protective equipment such as gloves, safety goggles, and lab coats should be worn to prevent skin contact and inhalation of vapors. Additionally, n-Hexane should only be used in a fume hood to minimize exposure to its vapors. Spills should be cleaned up promptly using absorbent materials and proper disposal procedures should be followed.
n-Hexane is a straight-chain alkane with the chemical formula C6H14. It is a common solvent used in various analytical techniques such as UV (ultraviolet), IR (infrared), and HPLC (high-performance liquid chromatography). In UV spectroscopy, n-Hexane is often used as a solvent for preparing samples or as a carrier solvent in mobile phases. In IR spectroscopy, n-Hexane can be used to dissolve nonpolar compounds for analysis. In HPLC, n-Hexane is utilized as a mobile phase solvent in reversed-phase chromatography.
How does n-Hexane perform in UV spectroscopy applications?
n-Hexane is commonly used in UV spectroscopy due to its UV-transparent nature in the range of 200-220 nm. This makes it ideal for analyzing compounds that absorb in this region. Additionally, n-Hexane has a low UV absorption which minimizes interference with the sample being analyzed. Its low viscosity and volatility make it a suitable solvent for UV spectroscopy applications.
What are the advantages of using n-Hexane in IR spectroscopy?
n-Hexane is a nonpolar solvent that is commonly used in IR spectroscopy for dissolving nonpolar compounds. Being nonpolar, n-Hexane interacts minimally with most organic compounds, ensuring that the IR spectra obtained are not distorted by solvent peaks. Its compatibility with a wide range of compounds makes it a versatile choice for IR spectroscopy applications. Additionally, n-Hexane is relatively easy to evaporate, leaving minimal residue on the sample surface.
How is n-Hexane used in HPLC applications?
In HPLC, n-Hexane is often used as a mobile phase solvent in reversed-phase chromatography. It is particularly useful for the separation of nonpolar compounds due to its low polarity. n-Hexane can be combined with other solvents such as methanol or acetonitrile to optimize the chromatographic separation of compounds with varying polarities. Its volatility and compatibility with common stationary phases make n-Hexane a popular choice for HPLC applications.
What precautions should be taken when handling n-Hexane in the laboratory?
n-Hexane is a highly flammable liquid that should be handled with care in the laboratory. It should be stored in a cool, well-ventilated area away from sources of ignition. When working with n-Hexane, proper personal protective equipment such as gloves, safety goggles, and lab coats should be worn to prevent skin contact and inhalation of vapors. Additionally, n-Hexane should only be used in a fume hood to minimize exposure to its vapors. Spills should be cleaned up promptly using absorbent materials and proper disposal procedures should be followed.
