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| Product Name | L-Glutamine for cell culture |
| Supplier | Multiple suppliers available |
| Form | Powder |
| Purity | 99% or higher |
| Storage | Store at room temperature |
| Solubility | Soluble in water |
| Usage | Used as a cell culture supplement |
| Appearance | White crystalline powder |
| Packaging | Available in various sizes |
| Shelf Life | 2 years |
| Applications | Supports cell growth and proliferation |
FAQ
What is L-Glutamine and why is it important for cell culture?
L-Glutamine is an amino acid that plays a crucial role in cell culture. It is essential for cell proliferation, metabolism, and maintaining cellular homeostasis. Without an adequate supply of L-Glutamine, cells may not grow or survive in culture.
How does L-Glutamine benefit cell culture?
L-Glutamine is a key source of energy for rapidly dividing cells, such as cancer cells or immune cells. It also serves as a precursor for the synthesis of nucleotides and proteins, which are essential for cell growth and function. In addition, L-Glutamine helps to buffer the pH of the cell culture medium, preventing harmful changes in acidity that can affect cell viability.
What are some common applications of L-Glutamine in cell culture?
L-Glutamine is commonly used in a variety of cell culture applications, including cell line maintenance, primary cell culture, stem cell culture, and bioproduction of recombinant proteins. It is also used in the production of viral vectors for gene therapy and vaccine development.
How should I add L-Glutamine to my cell culture media?
L-Glutamine is typically added to cell culture media at a final concentration of 2-4 mM. It is important to avoid excessive L-Glutamine concentrations, as this can lead to the accumulation of toxic ammonia in the culture medium. L-Glutamine should be added to the media right before use, as it is unstable in solution and can degrade over time.
Are there any alternatives to using L-Glutamine in cell culture?
While L-Glutamine is the most commonly used amino acid supplement in cell culture, some researchers have explored alternative strategies to provide cells with glutamine, such as using L-Glutamine analogs or replacing L-Glutamine with other amino acids. However, L-Glutamine remains the preferred choice for most cell culture applications due to its availability, stability, and proven track record of supporting cell growth and viability.
L-Glutamine is an amino acid that plays a crucial role in cell culture. It is essential for cell proliferation, metabolism, and maintaining cellular homeostasis. Without an adequate supply of L-Glutamine, cells may not grow or survive in culture.
How does L-Glutamine benefit cell culture?
L-Glutamine is a key source of energy for rapidly dividing cells, such as cancer cells or immune cells. It also serves as a precursor for the synthesis of nucleotides and proteins, which are essential for cell growth and function. In addition, L-Glutamine helps to buffer the pH of the cell culture medium, preventing harmful changes in acidity that can affect cell viability.
What are some common applications of L-Glutamine in cell culture?
L-Glutamine is commonly used in a variety of cell culture applications, including cell line maintenance, primary cell culture, stem cell culture, and bioproduction of recombinant proteins. It is also used in the production of viral vectors for gene therapy and vaccine development.
How should I add L-Glutamine to my cell culture media?
L-Glutamine is typically added to cell culture media at a final concentration of 2-4 mM. It is important to avoid excessive L-Glutamine concentrations, as this can lead to the accumulation of toxic ammonia in the culture medium. L-Glutamine should be added to the media right before use, as it is unstable in solution and can degrade over time.
Are there any alternatives to using L-Glutamine in cell culture?
While L-Glutamine is the most commonly used amino acid supplement in cell culture, some researchers have explored alternative strategies to provide cells with glutamine, such as using L-Glutamine analogs or replacing L-Glutamine with other amino acids. However, L-Glutamine remains the preferred choice for most cell culture applications due to its availability, stability, and proven track record of supporting cell growth and viability.
