G418 Disulfate BioChemica

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Name G418 Disulfate
Brand BioChemica
Product Type Biochemical
Cas Number 108321-43-6
Molecular Weight 692.7 g/mol
Purity ≥98%
Form Powder
Solubility Soluble in water
Storage Condition Store at -20°C
Applications Antibiotic selection in cell culture
Appearance White to off-white crystalline powder
Melting Point 240-245°C
Boiling Point N/A
Density N/A
Usage Laboratory research
Shelf Life 2 years
Packaging Available in various sizes
FAQ
What is G418 Disulfate BioChemica and how is it used in research studies?

G418 Disulfate BioChemica is a popular antibiotic commonly used in biotechnology and molecular biology research. It is often used in genetic selection experiments as a selective agent to kill cells that have been modified or transfected with a gene of interest. G418 disrupts protein synthesis in the ribosome, leading to cell death in cells that do not contain the gene resistant to G418. This antibiotic is essential for maintaining healthy and growing populations of transformed cells in various laboratory settings.

What are the advantages of using G418 Disulfate BioChemica in research studies?

G418 Disulfate BioChemica offers several advantages for researchers conducting genetic selection experiments. Firstly, it has a broad spectrum of activity, making it effective against a wide range of organisms. This versatility allows researchers to use G418 in various cell lines and species, making it a valuable tool for many different research applications. Additionally, G418 is stable in solution and retains its activity even after prolonged storage, providing consistent and reliable results for researchers. Lastly, G418 is relatively easy to use in laboratory settings, requiring simple preparation for use in cell culture experiments.

How should G418 Disulfate BioChemica be stored and handled in the laboratory?

Proper storage and handling of G418 Disulfate BioChemica are essential to maintain its stability and effectiveness in research settings. It is recommended to store the antibiotic in a cool, dry place away from direct light and heat. Additionally, G418 should be kept in its original packaging or a tightly sealed container to prevent contamination or degradation. When handling G418, researchers should wear appropriate personal protective equipment, such as gloves and goggles, to prevent skin or eye contact with the antibiotic. Proper disposal of any unused or expired G418 is also crucial to prevent environmental contamination.

Are there any safety precautions or potential hazards associated with using G418 Disulfate BioChemica in the laboratory?

While G418 Disulfate BioChemica is generally considered safe for use in research settings, there are some safety precautions that researchers should be aware of when handling the antibiotic. G418 can be irritating to the skin, eyes, and respiratory tract, so researchers should take precautions to avoid contact with the antibiotic. In the event of accidental exposure, researchers should immediately rinse the affected area with water and seek medical attention if necessary. Additionally, researchers should be aware of proper disposal procedures for G418 to minimize environmental impact. Overall, by following proper handling and storage protocols, researchers can minimize the risks associated with using G418 Disulfate BioChemica in the laboratory.

What are some potential applications of G418 Disulfate BioChemica in research studies?

G418 Disulfate BioChemica has a wide range of applications in biotechnology and molecular biology research. One common use of G418 is in the selection of genetically modified cells in culture. By introducing a gene resistant to G418 into cells, researchers can selectively kill off non-transfected cells, allowing for the isolation and study of the genetically modified population. G418 is also used in gene knockout experiments to inactivate specific genes of interest for functional studies. Additionally, G418 has applications in protein expression studies, where it can be used to select for cells that have successfully incorporated a gene encoding a protein of interest. Overall, G418 Disulfate BioChemica is a versatile tool with many potential applications in research studies.
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