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| Product Name | Hygromycin B |
| Product Type | Antibiotic |
| Target Species | Bacteria, fungi, and higher eukaryotic cells |
| Mode Of Action | Inhibits protein synthesis by binding to the 30S subunit of the ribosome |
| Selectivity | Broad-spectrum antibiotic |
| Effective Against | Gram-positive and Gram-negative bacteria, and certain eukaryotic cells |
| Mechanism Of Resistance | Expression of aminoglycoside-modifying enzymes |
| Solubility | Soluble in water |
| Storage Condition | Store at -20°C |
| Shelf Life | 2 years |
| Recommended Concentration | 50-200 µg/mL for selection in cell culture |
FAQ
What is Hygromycin B and how does it work?
Hygromycin B is an antibiotic that is commonly used in molecular biology research to select for cells that have been successfully transfected with a plasmid that has a hygromycin resistance gene. This antibiotic works by inhibiting protein synthesis in cells, which ultimately leads to cell death.
How is Hygromycin B typically used in research applications?
In research applications, Hygromycin B is most commonly used as a selective agent in cell culture media. After introducing a plasmid with a hygromycin resistance gene into cells, researchers can then add Hygromycin B to the cell culture media. Only cells that have successfully taken up the plasmid and expressed the resistance gene will survive in the presence of the antibiotic.
What are the benefits of using Hygromycin B in cell culture experiments?
One of the main benefits of using Hygromycin B in cell culture experiments is its high selectivity for cells that have been transfected with a hygromycin resistance gene. This allows researchers to easily select for and maintain cells that have incorporated the gene of interest. Additionally, Hygromycin B is stable in cell culture media and has a broad spectrum of activity against a wide range of cell types.
Are there any considerations or precautions to keep in mind when using Hygromycin B in research?
While Hygromycin B is generally considered safe to use in research applications, there are some precautions that researchers should be aware of. It is important to use proper protective equipment when handling Hygromycin B, as it can be toxic if it comes into contact with skin or eyes. Additionally, researchers should follow proper disposal procedures for any unused Hygromycin B and contaminated materials.
How can researchers obtain Hygromycin B for their experiments?
Researchers can typically purchase Hygromycin B from scientific suppliers that specialize in molecular biology reagents. It is important to ensure that the Hygromycin B purchased is of high quality and purity to ensure reliable results in experiments. Researchers should also follow the manufacturer's guidelines for storage and handling of the antibiotic to maintain its stability and efficacy.
Hygromycin B is an antibiotic that is commonly used in molecular biology research to select for cells that have been successfully transfected with a plasmid that has a hygromycin resistance gene. This antibiotic works by inhibiting protein synthesis in cells, which ultimately leads to cell death.
How is Hygromycin B typically used in research applications?
In research applications, Hygromycin B is most commonly used as a selective agent in cell culture media. After introducing a plasmid with a hygromycin resistance gene into cells, researchers can then add Hygromycin B to the cell culture media. Only cells that have successfully taken up the plasmid and expressed the resistance gene will survive in the presence of the antibiotic.
What are the benefits of using Hygromycin B in cell culture experiments?
One of the main benefits of using Hygromycin B in cell culture experiments is its high selectivity for cells that have been transfected with a hygromycin resistance gene. This allows researchers to easily select for and maintain cells that have incorporated the gene of interest. Additionally, Hygromycin B is stable in cell culture media and has a broad spectrum of activity against a wide range of cell types.
Are there any considerations or precautions to keep in mind when using Hygromycin B in research?
While Hygromycin B is generally considered safe to use in research applications, there are some precautions that researchers should be aware of. It is important to use proper protective equipment when handling Hygromycin B, as it can be toxic if it comes into contact with skin or eyes. Additionally, researchers should follow proper disposal procedures for any unused Hygromycin B and contaminated materials.
How can researchers obtain Hygromycin B for their experiments?
Researchers can typically purchase Hygromycin B from scientific suppliers that specialize in molecular biology reagents. It is important to ensure that the Hygromycin B purchased is of high quality and purity to ensure reliable results in experiments. Researchers should also follow the manufacturer's guidelines for storage and handling of the antibiotic to maintain its stability and efficacy.
