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| Name | Hygromycin B - Solution |
| Brand | Thermo Fisher Scientific |
| Quantity | 100 mL |
| Concentration | 50 mg/mL |
| Storage Condition | Store at -20°C |
| Usage | Antibiotic for selection in cell culture |
| Target Organisms | Bacteria, fungi, eukaryotic microorganisms |
| Selection Marker | Hygromycin resistance gene |
| Mode Of Action | Inhibits protein synthesis |
| Purity | ≥ 95% |
| Solubility | Soluble in water |
| Working Solution | Dilute to desired concentration in cell culture media |
| Shelf Life | 2 years |
| Appearance | Clear, colorless solution |
| Shipping Condition | Shipped on blue ice |
| Compatibility | Compatible with most commonly used cell culture media |
| Recommended Storage | Avoid repeated freeze-thaw cycles |
FAQ
What is Hygromycin B - Solution?
Hygromycin B - Solution is an antibiotic solution commonly used in cell culture applications to select for cells that have been transfected with a gene of interest.
How does Hygromycin B - Solution work?
Hygromycin B - Solution works by inhibiting protein synthesis in cells that do not possess the resistance gene for hygromycin. This allows only cells that have been successfully transfected with the gene of interest, which also contains the hygromycin resistance gene, to survive and proliferate in the culture.
What concentration of Hygromycin B - Solution should be used for selection in cell culture?
The optimal concentration of Hygromycin B - Solution for selection in cell culture can vary depending on the cell line and experimental conditions. It is recommended to perform a dose-response curve to determine the appropriate concentration for your specific application.
Is Hygromycin B - Solution compatible with all cell types?
While Hygromycin B - Solution is commonly used for selection in a wide range of cell lines, some cell types may be more sensitive to the antibiotic than others. It is important to optimize the concentration of Hygromycin B - Solution for each specific cell type to ensure effective selection without causing excessive cell death.
Can Hygromycin B - Solution be used in combination with other antibiotics or selection reagents?
Hygromycin B - Solution can be used in combination with other antibiotics or selection reagents, depending on the experimental requirements. However, it is important to ensure that the combination of reagents does not interfere with the selection process or compromise the viability of the cells.
Hygromycin B - Solution is an antibiotic solution commonly used in cell culture applications to select for cells that have been transfected with a gene of interest.
How does Hygromycin B - Solution work?
Hygromycin B - Solution works by inhibiting protein synthesis in cells that do not possess the resistance gene for hygromycin. This allows only cells that have been successfully transfected with the gene of interest, which also contains the hygromycin resistance gene, to survive and proliferate in the culture.
What concentration of Hygromycin B - Solution should be used for selection in cell culture?
The optimal concentration of Hygromycin B - Solution for selection in cell culture can vary depending on the cell line and experimental conditions. It is recommended to perform a dose-response curve to determine the appropriate concentration for your specific application.
Is Hygromycin B - Solution compatible with all cell types?
While Hygromycin B - Solution is commonly used for selection in a wide range of cell lines, some cell types may be more sensitive to the antibiotic than others. It is important to optimize the concentration of Hygromycin B - Solution for each specific cell type to ensure effective selection without causing excessive cell death.
Can Hygromycin B - Solution be used in combination with other antibiotics or selection reagents?
Hygromycin B - Solution can be used in combination with other antibiotics or selection reagents, depending on the experimental requirements. However, it is important to ensure that the combination of reagents does not interfere with the selection process or compromise the viability of the cells.
