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| Name | Penicillin - Streptomycin (100X) for cell culture |
| Concentration | 100X |
| Components | Penicillin, Streptomycin |
| Application | Cell culture |
| Formulation | Liquid |
| Storage Conditions | Store at -20°C |
| Shelf Life | 2 years |
| Sterility | Sterile filtered |
| Endotoxin Level | Less than 0.1 EU/ml |
| Usage Recommendation | Dilute 1:100 before adding to cell culture |
| Supplier | Multiple suppliers available |
| Packaging Size | Various sizes available |
| Appearance | Clear, colorless solution |
| Suitable For | Research and cell culture applications |
FAQ
What is Penicillin-Streptomycin (100X) and how is it used in cell
culture?
Penicillin-Streptomycin (100X) is a commonly used antibiotic solution in cell culture. It contains a mixture of penicillin and streptomycin, two antibiotics that work together to prevent bacterial contamination in cell culture experiments. Penicillin inhibits the synthesis of the bacterial cell wall, while streptomycin interferes with protein synthesis in bacteria. By adding this antibiotic solution to cell culture media, researchers can protect their cells from bacterial infections and ensure the purity of their experiments.
Why is it important to use Penicillin-Streptomycin in cell culture?
Maintaining a sterile environment is crucial in cell culture experiments to prevent contamination and ensure the validity of results. Bacterial contamination can negatively impact cell growth, viability, and overall experimental outcomes. By using Penicillin-Streptomycin (100X) in cell culture, researchers can minimize the risk of bacterial contamination and maintain the integrity of their cell culture experiments. This antibiotic solution provides an effective way to protect cells from unwanted microbial growth and ensure the success of cell culture studies.
How should Penicillin-Streptomycin be added to cell culture media?
Penicillin-Streptomycin (100X) should be added to cell culture media at the recommended concentration of 1% (v/v), which is equivalent to a final concentration of 100 units/mL of penicillin and 100 μg/mL of streptomycin. This concentration is effective in preventing bacterial contamination without causing harm to the cultured cells. Researchers should carefully calculate and measure the appropriate volume of Penicillin-Streptomycin solution to achieve the desired concentration in their cell culture media. It is important to handle the antibiotic solution with care to avoid contamination and ensure its effectiveness in protecting cells.
Are there any considerations or precautions to keep in mind when using Penicillin-Streptomycin in cell culture?
While Penicillin-Streptomycin (100X) is a commonly used antibiotic solution in cell culture, researchers should be aware of potential interactions or sensitivities that certain cell types may have to these antibiotics. Some cells may be sensitive to penicillin or streptomycin, leading to decreased cell viability or altered growth patterns. It is recommended to perform a sensitivity test with a small number of cells before adding Penicillin-Streptomycin to a large cell culture batch. Additionally, researchers should regularly monitor their cell cultures for signs of contamination or adverse effects when using antibiotics. If any issues arise, adjustments to the antibiotic concentration or alternative antibacterial agents may be necessary.
Can Penicillin-Streptomycin (100X) be used for long-term cell culture experiments?
Penicillin-Streptomycin (100X) is suitable for short to medium-term cell culture experiments, typically ranging from a few days to a few weeks. However, for long-term cell culture studies or experiments requiring extended passages, the continuous presence of antibiotics in the media may affect cell behavior and gene expression. In such cases, researchers may consider using alternative methods for preventing bacterial contamination, such as periodic antibiotic treatment or implementing strict aseptic techniques. It is essential to weigh the benefits of bacterial protection against the potential impact on cell physiology when using antibiotics in long-term cell culture experiments.
Penicillin-Streptomycin (100X) is a commonly used antibiotic solution in cell culture. It contains a mixture of penicillin and streptomycin, two antibiotics that work together to prevent bacterial contamination in cell culture experiments. Penicillin inhibits the synthesis of the bacterial cell wall, while streptomycin interferes with protein synthesis in bacteria. By adding this antibiotic solution to cell culture media, researchers can protect their cells from bacterial infections and ensure the purity of their experiments.
Why is it important to use Penicillin-Streptomycin in cell culture?
Maintaining a sterile environment is crucial in cell culture experiments to prevent contamination and ensure the validity of results. Bacterial contamination can negatively impact cell growth, viability, and overall experimental outcomes. By using Penicillin-Streptomycin (100X) in cell culture, researchers can minimize the risk of bacterial contamination and maintain the integrity of their cell culture experiments. This antibiotic solution provides an effective way to protect cells from unwanted microbial growth and ensure the success of cell culture studies.
How should Penicillin-Streptomycin be added to cell culture media?
Penicillin-Streptomycin (100X) should be added to cell culture media at the recommended concentration of 1% (v/v), which is equivalent to a final concentration of 100 units/mL of penicillin and 100 μg/mL of streptomycin. This concentration is effective in preventing bacterial contamination without causing harm to the cultured cells. Researchers should carefully calculate and measure the appropriate volume of Penicillin-Streptomycin solution to achieve the desired concentration in their cell culture media. It is important to handle the antibiotic solution with care to avoid contamination and ensure its effectiveness in protecting cells.
Are there any considerations or precautions to keep in mind when using Penicillin-Streptomycin in cell culture?
While Penicillin-Streptomycin (100X) is a commonly used antibiotic solution in cell culture, researchers should be aware of potential interactions or sensitivities that certain cell types may have to these antibiotics. Some cells may be sensitive to penicillin or streptomycin, leading to decreased cell viability or altered growth patterns. It is recommended to perform a sensitivity test with a small number of cells before adding Penicillin-Streptomycin to a large cell culture batch. Additionally, researchers should regularly monitor their cell cultures for signs of contamination or adverse effects when using antibiotics. If any issues arise, adjustments to the antibiotic concentration or alternative antibacterial agents may be necessary.
Can Penicillin-Streptomycin (100X) be used for long-term cell culture experiments?
Penicillin-Streptomycin (100X) is suitable for short to medium-term cell culture experiments, typically ranging from a few days to a few weeks. However, for long-term cell culture studies or experiments requiring extended passages, the continuous presence of antibiotics in the media may affect cell behavior and gene expression. In such cases, researchers may consider using alternative methods for preventing bacterial contamination, such as periodic antibiotic treatment or implementing strict aseptic techniques. It is essential to weigh the benefits of bacterial protection against the potential impact on cell physiology when using antibiotics in long-term cell culture experiments.
