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| Product Name | Puromycin Dihydrochloride BioChemica |
| CAS Number | 58-58-2 |
| Synonyms | Puromycin hydrochloride, CL-50182A |
| Molecular Formula | C22H29N7O5 · 2HCl |
| Molecular Weight | 544.46 g/mol |
| Appearance | White to off-white crystalline powder |
| Solubility | Soluble in water |
| Storage Conditions | Store at -20°C |
| Usage | Antibiotic protein synthesis inhibitor |
| Purity | ≥98% |
| Handling | Avoid exposure - obtain special instructions before use |
FAQ
What is Puromycin Dihydrochloride BioChemica and how does it work?
Puromycin Dihydrochloride BioChemica is a selective antibiotic compound that is used in molecular biology to inhibit protein synthesis in both prokaryotic and eukaryotic cells. It works by binding to the ribosome and causing premature chain termination during translation, ultimately leading to the inhibition of protein synthesis.
How is Puromycin Dihydrochloride BioChemica typically used in research?
Puromycin Dihydrochloride BioChemica is commonly used in research to select for cells that have been transfected with a plasmid containing a puromycin resistance gene. The cells are treated with puromycin, which kills off those that have not been successfully transfected. This allows researchers to easily identify and isolate the cells that have taken up the plasmid.
What are the benefits of using Puromycin Dihydrochloride BioChemica in research?
One of the main benefits of using Puromycin Dihydrochloride BioChemica in research is its high selectivity for actively translating ribosomes, which allows for the efficient inhibition of protein synthesis in cells. Additionally, it is relatively stable and easy to use, making it a popular choice for many researchers.
Are there any considerations or precautions to take when using Puromycin Dihydrochloride BioChemica?
Yes, there are several precautions that should be taken when using Puromycin Dihydrochloride BioChemica. It is important to handle the compound with care as it can be toxic if ingested or absorbed through the skin. Additionally, researchers should be aware of the proper dosages and treatment times to ensure the optimal results while minimizing potential negative effects on the cells.
What are some potential applications of Puromycin Dihydrochloride BioChemica outside of research?
While Puromycin Dihydrochloride BioChemica is primarily used in research settings, it also has potential applications in biotechnology and pharmaceutical industries. For example, it could be used to study protein synthesis in cell-free systems or to produce proteins of interest in recombinant protein expression systems. Overall, Puromycin Dihydrochloride BioChemica is a versatile compound with a wide range of potential applications.
Puromycin Dihydrochloride BioChemica is a selective antibiotic compound that is used in molecular biology to inhibit protein synthesis in both prokaryotic and eukaryotic cells. It works by binding to the ribosome and causing premature chain termination during translation, ultimately leading to the inhibition of protein synthesis.
How is Puromycin Dihydrochloride BioChemica typically used in research?
Puromycin Dihydrochloride BioChemica is commonly used in research to select for cells that have been transfected with a plasmid containing a puromycin resistance gene. The cells are treated with puromycin, which kills off those that have not been successfully transfected. This allows researchers to easily identify and isolate the cells that have taken up the plasmid.
What are the benefits of using Puromycin Dihydrochloride BioChemica in research?
One of the main benefits of using Puromycin Dihydrochloride BioChemica in research is its high selectivity for actively translating ribosomes, which allows for the efficient inhibition of protein synthesis in cells. Additionally, it is relatively stable and easy to use, making it a popular choice for many researchers.
Are there any considerations or precautions to take when using Puromycin Dihydrochloride BioChemica?
Yes, there are several precautions that should be taken when using Puromycin Dihydrochloride BioChemica. It is important to handle the compound with care as it can be toxic if ingested or absorbed through the skin. Additionally, researchers should be aware of the proper dosages and treatment times to ensure the optimal results while minimizing potential negative effects on the cells.
What are some potential applications of Puromycin Dihydrochloride BioChemica outside of research?
While Puromycin Dihydrochloride BioChemica is primarily used in research settings, it also has potential applications in biotechnology and pharmaceutical industries. For example, it could be used to study protein synthesis in cell-free systems or to produce proteins of interest in recombinant protein expression systems. Overall, Puromycin Dihydrochloride BioChemica is a versatile compound with a wide range of potential applications.
