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| Name | Agarose Low EEO (Agarose Standard) |
| Type | Agarose gel |
| Purity | High purity |
| Gel Strength | Standard |
| EOE | Low EEO (Electroendosmosis) |
| Molecular Weight | Medium |
| Gel Temperature | Standard |
| Gel Concentration | Standard |
| Gel Resolution | Good resolution |
| Applications | Electrophoresis, DNA/RNA analysis |
| Storage Conditions | Room temperature |
| Shelf Life | 2 years |
| Packaging | Available in various pack sizes |
| Brand | Multiple brands available |
| Color | Off-white |
| Solubility | Soluble in water |
| Melting Temperature | Standard |
| Shipping Information | Shipped at ambient temperature |
| Safety Information | Avoid inhalation, ingestion, and contact with eyes |
| Regulatory Compliance | Manufactured in accordance with ISO guidelines |
FAQ
What is Agarose low EEO (Agarose Standard)?
Agarose low EEO (Agarose Standard) is a high-quality, standard agarose powder used for gel electrophoresis and other molecular biology applications. This agarose has a low electroendosmosis (EEO) value, making it ideal for separating and analyzing nucleic acids and proteins in a gel matrix.
What are the benefits of using Agarose low EEO (Agarose Standard)?
Agarose low EEO offers several advantages for researchers, including:
- High gel strength, allowing for easy handling and minimal breakage during gel casting and handling.
- Low EEO value, reducing the risk of artifacts in electrophoresis and providing clear, sharp bands for accurate analysis.
- High purity and low DNA binding, ensuring minimal interference with sample migration and detection.
- Wide range of resolution, suitable for separating a variety of DNA fragments or PCR products.
How is Agarose low EEO (Agarose Standard) used in gel electrophoresis?
Agarose low EEO is typically mixed with a buffer solution, heated to dissolve the agarose, and then poured into a gel casting tray with a comb to create wells for sample loading. Once the gel has solidified, samples containing nucleic acids or proteins are loaded into the wells, and an electric current is applied to separate the molecules based on their size and charge. The agarose gel matrix acts as a sieve, allowing smaller molecules to migrate faster through the gel than larger ones.
What concentration of Agarose low EEO (Agarose Standard) should be used for gel electrophoresis?
The optimal agarose concentration for gel electrophoresis depends on the size range of the nucleic acids or proteins being analyzed. Typically, agarose concentrations ranging from 0.5% to 2% are used for DNA fragment separation, with lower percentages providing better resolution for smaller fragments and higher percentages for larger fragments. It is recommended to perform a test run with a range of concentrations to determine the optimal agarose concentration for your specific application.
How should Agarose low EEO (Agarose Standard) gels be stained and visualized after electrophoresis?
After gel electrophoresis is complete, Agarose low EEO gels can be stained with a dye that binds to nucleic acids or proteins to visualize the bands. Common stains include ethidium bromide, SYBR Safe, or GelRed, which fluoresce under UV light and allow for the detection of DNA or protein bands. The stained gel can be photographed or viewed under UV light using a gel documentation system for analysis and documentation of results.
Agarose low EEO (Agarose Standard) is a high-quality, standard agarose powder used for gel electrophoresis and other molecular biology applications. This agarose has a low electroendosmosis (EEO) value, making it ideal for separating and analyzing nucleic acids and proteins in a gel matrix.
What are the benefits of using Agarose low EEO (Agarose Standard)?
Agarose low EEO offers several advantages for researchers, including:
- High gel strength, allowing for easy handling and minimal breakage during gel casting and handling.
- Low EEO value, reducing the risk of artifacts in electrophoresis and providing clear, sharp bands for accurate analysis.
- High purity and low DNA binding, ensuring minimal interference with sample migration and detection.
- Wide range of resolution, suitable for separating a variety of DNA fragments or PCR products.
How is Agarose low EEO (Agarose Standard) used in gel electrophoresis?
Agarose low EEO is typically mixed with a buffer solution, heated to dissolve the agarose, and then poured into a gel casting tray with a comb to create wells for sample loading. Once the gel has solidified, samples containing nucleic acids or proteins are loaded into the wells, and an electric current is applied to separate the molecules based on their size and charge. The agarose gel matrix acts as a sieve, allowing smaller molecules to migrate faster through the gel than larger ones.
What concentration of Agarose low EEO (Agarose Standard) should be used for gel electrophoresis?
The optimal agarose concentration for gel electrophoresis depends on the size range of the nucleic acids or proteins being analyzed. Typically, agarose concentrations ranging from 0.5% to 2% are used for DNA fragment separation, with lower percentages providing better resolution for smaller fragments and higher percentages for larger fragments. It is recommended to perform a test run with a range of concentrations to determine the optimal agarose concentration for your specific application.
How should Agarose low EEO (Agarose Standard) gels be stained and visualized after electrophoresis?
After gel electrophoresis is complete, Agarose low EEO gels can be stained with a dye that binds to nucleic acids or proteins to visualize the bands. Common stains include ethidium bromide, SYBR Safe, or GelRed, which fluoresce under UV light and allow for the detection of DNA or protein bands. The stained gel can be photographed or viewed under UV light using a gel documentation system for analysis and documentation of results.
