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Product Name | Ethidium Bromide - Solution 0.07% |
Packaging | Dropper bottle |
Concentration | 0.07% |
Volume | Varies based on bottle size |
Storage Conditions | Store at room temperature |
Appearance | Dark red liquid |
Solubility | Soluble in water |
Applications | Commonly used in DNA/RNA analysis and visualization |
Hazardous | Yes, handle with caution |
Shelf Life | 1-2 years when stored properly |
Manufacturer | Varies |
Country Of Origin | Varies |
Chemical Formula | C21H20BrN3 |
Shipping Restrictions | May have restrictions due to hazardous nature |
Usage | For laboratory research purposes only |
Additional Information | Refer to MSDS before use |
FAQ
What is Ethidium Bromide, and what is its role in molecular biology research?
Ethidium Bromide is a nucleic acid binding dye commonly used in molecular biology research for staining DNA and RNA in agarose gels. It is a fluorescent intercalating agent that binds to double-stranded DNA by intercalating between the bases, allowing for the visualization of nucleic acids under UV light.
How is Ethidium Bromide Solution 0.07% typically used in the laboratory setting?
Ethidium Bromide Solution 0.07% is typically used in the laboratory setting for staining DNA or RNA in agarose gels during electrophoresis. After running the gel, the gel is then stained with Ethidium Bromide Solution and visualized under UV light to visualize the bands of DNA or RNA.
What are the advantages of using Ethidium Bromide Solution 0.07% in research applications?
One of the main advantages of using Ethidium Bromide Solution 0.07% is its high sensitivity and ability to detect even small amounts of DNA or RNA. It is also relatively inexpensive and easy to use, making it a popular choice for researchers in molecular biology.
Are there any safety considerations when working with Ethidium Bromide Solution 0.07%?
Yes, Ethidium Bromide is a mutagen and potential carcinogen, so it is important to handle it with caution and follow proper safety protocols when working with it in the laboratory. This includes wearing appropriate personal protective equipment such as gloves and a lab coat, as well as disposing of any waste containing Ethidium Bromide properly.
Can Ethidium Bromide Solution 0.07% be stored long-term, and what are the recommended storage conditions?
Ethidium Bromide Solution 0.07% should be stored in a dark container at room temperature, away from light and heat sources, to prevent degradation. It is recommended to check the expiration date on the bottle and discard any expired solution to ensure optimal performance when using it in the laboratory.
Ethidium Bromide is a nucleic acid binding dye commonly used in molecular biology research for staining DNA and RNA in agarose gels. It is a fluorescent intercalating agent that binds to double-stranded DNA by intercalating between the bases, allowing for the visualization of nucleic acids under UV light.
How is Ethidium Bromide Solution 0.07% typically used in the laboratory setting?
Ethidium Bromide Solution 0.07% is typically used in the laboratory setting for staining DNA or RNA in agarose gels during electrophoresis. After running the gel, the gel is then stained with Ethidium Bromide Solution and visualized under UV light to visualize the bands of DNA or RNA.
What are the advantages of using Ethidium Bromide Solution 0.07% in research applications?
One of the main advantages of using Ethidium Bromide Solution 0.07% is its high sensitivity and ability to detect even small amounts of DNA or RNA. It is also relatively inexpensive and easy to use, making it a popular choice for researchers in molecular biology.
Are there any safety considerations when working with Ethidium Bromide Solution 0.07%?
Yes, Ethidium Bromide is a mutagen and potential carcinogen, so it is important to handle it with caution and follow proper safety protocols when working with it in the laboratory. This includes wearing appropriate personal protective equipment such as gloves and a lab coat, as well as disposing of any waste containing Ethidium Bromide properly.
Can Ethidium Bromide Solution 0.07% be stored long-term, and what are the recommended storage conditions?
Ethidium Bromide Solution 0.07% should be stored in a dark container at room temperature, away from light and heat sources, to prevent degradation. It is recommended to check the expiration date on the bottle and discard any expired solution to ensure optimal performance when using it in the laboratory.