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| Product Name | Ficoll® 400 BioChemica |
| Brand | BioChemica |
| Type | Cell Separation and Isolation Reagent |
| Grade | Research Grade |
| Form | Powder |
| Molecular Weight | ≈400 kDa |
| Density | 1.26-1.29 g/mL at 20°C |
| Solubility | Soluble in water |
| Storage Conditions | Store at room temperature |
| Shelf Life | 2 years |
| CAS Number | 400 DCC |
| Function | Used for density gradient centrifugation |
| Applications | Cell isolation, purification, and separation |
| Appearance | White to off-white powder |
| Purity | ≥95% |
| Specific Gravity | 1.26-1.29 |
| Packaging | Available in various sizes |
| Stability | Stable under recommended storage conditions |
| Country Of Origin | Germany |
| UN Number | Not applicable |
| UN Proper Shipping Name | Not applicable |
FAQ
What is Ficoll® 400 BioChemica used for?
Ficoll® 400 BioChemica is commonly used in biochemistry and molecular biology applications for density gradient centrifugation, cell separation, and purification of subcellular organelles.
How does Ficoll® 400 BioChemica work in density gradient centrifugation?
Ficoll® 400 BioChemica forms a density gradient when dissolved in an appropriate buffer solution. During centrifugation, particles or cells are separated based on their buoyant density, allowing for efficient purification and isolation of different components.
What are the advantages of using Ficoll® 400 BioChemica for cell separation?
Ficoll® 400 BioChemica offers several advantages for cell separation, including high purity and yield of isolated cells, minimal impact on cell viability and functionality, and compatibility with various cell types and applications.
Can Ficoll® 400 BioChemica be used for isolating subcellular organelles?
Yes, Ficoll® 400 BioChemica is commonly used for isolating subcellular organelles due to its ability to form stable density gradients that facilitate the purification of organelles such as mitochondria, lysosomes, and peroxisomes.
Are there any specific storage or handling requirements for Ficoll® 400 BioChemica?
Ficoll® 400 BioChemica should be stored in a cool, dry place away from direct sunlight and moisture. It is important to follow the manufacturer's instructions for handling and preparation to ensure optimal performance and results.
Ficoll® 400 BioChemica is commonly used in biochemistry and molecular biology applications for density gradient centrifugation, cell separation, and purification of subcellular organelles.
How does Ficoll® 400 BioChemica work in density gradient centrifugation?
Ficoll® 400 BioChemica forms a density gradient when dissolved in an appropriate buffer solution. During centrifugation, particles or cells are separated based on their buoyant density, allowing for efficient purification and isolation of different components.
What are the advantages of using Ficoll® 400 BioChemica for cell separation?
Ficoll® 400 BioChemica offers several advantages for cell separation, including high purity and yield of isolated cells, minimal impact on cell viability and functionality, and compatibility with various cell types and applications.
Can Ficoll® 400 BioChemica be used for isolating subcellular organelles?
Yes, Ficoll® 400 BioChemica is commonly used for isolating subcellular organelles due to its ability to form stable density gradients that facilitate the purification of organelles such as mitochondria, lysosomes, and peroxisomes.
Are there any specific storage or handling requirements for Ficoll® 400 BioChemica?
Ficoll® 400 BioChemica should be stored in a cool, dry place away from direct sunlight and moisture. It is important to follow the manufacturer's instructions for handling and preparation to ensure optimal performance and results.
