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| Name | Formamide deionized for molecular biology |
| Brand | Sigma-Aldrich |
| Catalog Number | F9037 |
| Volume | 100 mL |
| Concentration | ≥99% |
| Purity Grade | Molecular biology grade |
| Appearance | Clear, colorless liquid |
| Storage Condition | Store at room temperature |
| Usage | Commonly used in molecular biology applications such as nucleic acid denaturation |
| Specificity | Deionized formamide eliminates the need for additional purification steps |
| Packaging | Glass bottle |
| Solubility | Soluble in water |
| Boiling Point | 210-212°C |
| Flash Point | 168°C |
| UN Number | 1993 |
| Hazard Statements | Causes skin irritation. Causes serious eye damage. May cause respiratory irritation. |
| Safety Precautions | Wear protective gloves/protective clothing/eye protection/face protection. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
FAQ
What is Formamide deionized, and how is it used in molecular biology?
Formamide deionized is a chemical reagent commonly used in molecular biology research. It is often employed in procedures such as hybridization, RNA sequencing, and DNA sequencing due to its ability to denature nucleic acids. By disrupting the hydrogen bonds that hold the molecules together, formamide deionized helps to separate the two strands of DNA or RNA, allowing for analysis and manipulation.
Why is deionized formamide preferred over regular formamide for molecular biology applications?
Deionized formamide is preferred over regular formamide for molecular biology applications because it lacks impurities that can interfere with experimental results. Regular formamide may contain trace amounts of salts and other contaminants that could affect the outcome of experiments, while deionized formamide has been purified to ensure high quality and reliability.
What are some common protocols that require the use of deionized formamide in molecular biology?
Deionized formamide is commonly used in molecular biology protocols that involve nucleic acid analysis and manipulation. Some examples of procedures that require the use of deionized formamide include in situ hybridization, Northern blotting, and fluorescent in situ hybridization (FISH). By incorporating deionized formamide into these protocols, researchers can achieve accurate and reproducible results.
How should deionized formamide be stored to maintain its stability and effectiveness?
Deionized formamide should be stored at room temperature in a tightly sealed container to prevent exposure to moisture and contaminants. It is important to avoid extreme temperatures, as well as exposure to light, which can degrade the quality of the reagent. By following proper storage guidelines, researchers can ensure that their deionized formamide remains stable and effective for molecular biology applications.
What are some tips for working with deionized formamide in the laboratory?
When working with deionized formamide in the laboratory, it is important to handle the reagent with care to avoid exposure to skin or eyes. Protective gloves, goggles, and a lab coat should be worn at all times when handling deionized formamide. Additionally, it is recommended to work in a well-ventilated area to avoid inhaling any fumes that may be released during use. By following these safety guidelines, researchers can safely and effectively incorporate deionized formamide into their molecular biology experiments.
Formamide deionized is a chemical reagent commonly used in molecular biology research. It is often employed in procedures such as hybridization, RNA sequencing, and DNA sequencing due to its ability to denature nucleic acids. By disrupting the hydrogen bonds that hold the molecules together, formamide deionized helps to separate the two strands of DNA or RNA, allowing for analysis and manipulation.
Why is deionized formamide preferred over regular formamide for molecular biology applications?
Deionized formamide is preferred over regular formamide for molecular biology applications because it lacks impurities that can interfere with experimental results. Regular formamide may contain trace amounts of salts and other contaminants that could affect the outcome of experiments, while deionized formamide has been purified to ensure high quality and reliability.
What are some common protocols that require the use of deionized formamide in molecular biology?
Deionized formamide is commonly used in molecular biology protocols that involve nucleic acid analysis and manipulation. Some examples of procedures that require the use of deionized formamide include in situ hybridization, Northern blotting, and fluorescent in situ hybridization (FISH). By incorporating deionized formamide into these protocols, researchers can achieve accurate and reproducible results.
How should deionized formamide be stored to maintain its stability and effectiveness?
Deionized formamide should be stored at room temperature in a tightly sealed container to prevent exposure to moisture and contaminants. It is important to avoid extreme temperatures, as well as exposure to light, which can degrade the quality of the reagent. By following proper storage guidelines, researchers can ensure that their deionized formamide remains stable and effective for molecular biology applications.
What are some tips for working with deionized formamide in the laboratory?
When working with deionized formamide in the laboratory, it is important to handle the reagent with care to avoid exposure to skin or eyes. Protective gloves, goggles, and a lab coat should be worn at all times when handling deionized formamide. Additionally, it is recommended to work in a well-ventilated area to avoid inhaling any fumes that may be released during use. By following these safety guidelines, researchers can safely and effectively incorporate deionized formamide into their molecular biology experiments.
