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| Name | Immersion Oil for microscopy, fluorescence-free |
| Type | Microscopy consumable |
| Attributes | N/A |
FAQ
What is immersion oil and why is it important for microscopy?
Immersion oil is a type of oil used in microscopy to improve the resolution and clarity of images. It is essential for achieving high-quality results when using high-powered lenses. By placing a drop of immersion oil on the specimen and using an immersion lens, light can pass through the oil and directly into the lens, minimizing the refraction and reflection of light. This results in sharper and clearer images with increased magnification.
How does fluorescence-free immersion oil differ from regular immersion oil?
Fluorescence-free immersion oil is specially designed to reduce interference from fluorescence when using fluorescence microscopy techniques. Regular immersion oil may contain fluorescent impurities that can lead to unwanted background fluorescence in the images. Fluorescence-free immersion oil eliminates these impurities, allowing for more accurate and reliable results in fluorescence microscopy.
Can fluorescence-free immersion oil be used with other microscopy techniques?
Yes, fluorescence-free immersion oil can be used with a wide range of microscopy techniques, not just fluorescence microscopy. It is compatible with brightfield, phase contrast, differential interference contrast (DIC), and other common microscopy techniques. Its high optical clarity and low autofluorescence make it suitable for various applications where precise imaging is required.
What are the advantages of using fluorescence-free immersion oil over regular immersion oil?
The main advantage of using fluorescence-free immersion oil is the elimination of unwanted fluorescence signals that can compromise the quality of microscopy images. By using fluorescence-free immersion oil, researchers can obtain clearer, more detailed images without the interference of background fluorescence. This is especially beneficial for studies that require high sensitivity and specificity, such as live-cell imaging or immunofluorescence staining.
How should fluorescence-free immersion oil be stored and handled to ensure optimal performance?
Fluorescence-free immersion oil should be stored in a cool, dry place away from direct sunlight and extreme temperatures. It is important to keep the cap tightly closed when not in use to prevent contamination. Before each use, the oil should be inspected for any signs of cloudiness or impurities, and discarded if necessary. When handling the oil, it is recommended to use clean, lint-free applicators to avoid introducing any particles that could affect the quality of the images.
Immersion oil is a type of oil used in microscopy to improve the resolution and clarity of images. It is essential for achieving high-quality results when using high-powered lenses. By placing a drop of immersion oil on the specimen and using an immersion lens, light can pass through the oil and directly into the lens, minimizing the refraction and reflection of light. This results in sharper and clearer images with increased magnification.
How does fluorescence-free immersion oil differ from regular immersion oil?
Fluorescence-free immersion oil is specially designed to reduce interference from fluorescence when using fluorescence microscopy techniques. Regular immersion oil may contain fluorescent impurities that can lead to unwanted background fluorescence in the images. Fluorescence-free immersion oil eliminates these impurities, allowing for more accurate and reliable results in fluorescence microscopy.
Can fluorescence-free immersion oil be used with other microscopy techniques?
Yes, fluorescence-free immersion oil can be used with a wide range of microscopy techniques, not just fluorescence microscopy. It is compatible with brightfield, phase contrast, differential interference contrast (DIC), and other common microscopy techniques. Its high optical clarity and low autofluorescence make it suitable for various applications where precise imaging is required.
What are the advantages of using fluorescence-free immersion oil over regular immersion oil?
The main advantage of using fluorescence-free immersion oil is the elimination of unwanted fluorescence signals that can compromise the quality of microscopy images. By using fluorescence-free immersion oil, researchers can obtain clearer, more detailed images without the interference of background fluorescence. This is especially beneficial for studies that require high sensitivity and specificity, such as live-cell imaging or immunofluorescence staining.
How should fluorescence-free immersion oil be stored and handled to ensure optimal performance?
Fluorescence-free immersion oil should be stored in a cool, dry place away from direct sunlight and extreme temperatures. It is important to keep the cap tightly closed when not in use to prevent contamination. Before each use, the oil should be inspected for any signs of cloudiness or impurities, and discarded if necessary. When handling the oil, it is recommended to use clean, lint-free applicators to avoid introducing any particles that could affect the quality of the images.
