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| Product Name | Magnesium Chloride 100 mmol/L (100 mM) |
| Concentration | 100 mmol/L (100 mM) |
| Appearance | Clear colorless solution |
| Storage Conditions | Store at room temperature |
| Solubility | Soluble in water |
| PH Range | 6.0 - 7.0 |
| Purity | ≥ 99% |
| Application | Used as a cofactor in various enzymatic reactions |
| Stability | Stable for at least 1 year when stored properly |
| Hazards | Irritant to eyes and skin, harmful if ingested |
| Handling Precautions | Use appropriate protective equipment when handling |
FAQ
What is Magnesium Chloride 100 mmol/L (100 mM) used for in molecular biology?
Magnesium Chloride 100 mmol/L (100 mM) is commonly used as a cofactor in various enzymatic reactions in molecular biology, particularly in PCR (polymerase chain reaction) and other DNA manipulation techniques. It helps to stabilize DNA polymerases and optimize their activity, leading to more efficient and reliable amplification of target DNA sequences.
How does Magnesium Chloride 100 mmol/L (100 mM) benefit molecular biology research?
Magnesium Chloride 100 mmol/L (100 mM) plays a crucial role in many molecular biology experiments by facilitating the binding of primers to the template DNA and enhancing the fidelity of DNA synthesis. It also helps to maintain the stability of secondary structures in DNA molecules, thereby promoting successful PCR amplification and other molecular biology processes.
What are the advantages of using Magnesium Chloride 100 mmol/L (100 mM) over other magnesium sources in molecular biology applications?
Magnesium Chloride 100 mmol/L (100 mM) offers several advantages over other magnesium sources commonly used in molecular biology. It provides a standardized concentration of magnesium ions, ensuring reproducibility and consistency in experimental results. Additionally, magnesium chloride is less prone to form insoluble precipitates compared to magnesium sulfate, making it easier to handle and less likely to cause pipetting errors.
Can Magnesium Chloride 100 mmol/L (100 mM) be used in combination with other buffer components in molecular biology experiments?
Yes, Magnesium Chloride 100 mmol/L (100 mM) can be used in conjunction with various buffer components such as Tris-HCl, KCl, and dNTPs to create a suitable reaction environment for DNA amplification and other molecular biology techniques. It is essential to optimize the concentration of magnesium chloride along with other reagents to achieve optimal results in specific experimental conditions.
How should Magnesium Chloride 100 mmol/L (100 mM) be stored and handled to maintain its stability and effectiveness?
Magnesium Chloride 100 mmol/L (100 mM) should be stored at room temperature in a dry and cool environment to prevent moisture absorption and degradation. It is crucial to handle the solution with proper pipetting techniques to avoid contamination and ensure accurate dispensing of the desired magnesium chloride concentration in molecular biology experiments.
Magnesium Chloride 100 mmol/L (100 mM) is commonly used as a cofactor in various enzymatic reactions in molecular biology, particularly in PCR (polymerase chain reaction) and other DNA manipulation techniques. It helps to stabilize DNA polymerases and optimize their activity, leading to more efficient and reliable amplification of target DNA sequences.
How does Magnesium Chloride 100 mmol/L (100 mM) benefit molecular biology research?
Magnesium Chloride 100 mmol/L (100 mM) plays a crucial role in many molecular biology experiments by facilitating the binding of primers to the template DNA and enhancing the fidelity of DNA synthesis. It also helps to maintain the stability of secondary structures in DNA molecules, thereby promoting successful PCR amplification and other molecular biology processes.
What are the advantages of using Magnesium Chloride 100 mmol/L (100 mM) over other magnesium sources in molecular biology applications?
Magnesium Chloride 100 mmol/L (100 mM) offers several advantages over other magnesium sources commonly used in molecular biology. It provides a standardized concentration of magnesium ions, ensuring reproducibility and consistency in experimental results. Additionally, magnesium chloride is less prone to form insoluble precipitates compared to magnesium sulfate, making it easier to handle and less likely to cause pipetting errors.
Can Magnesium Chloride 100 mmol/L (100 mM) be used in combination with other buffer components in molecular biology experiments?
Yes, Magnesium Chloride 100 mmol/L (100 mM) can be used in conjunction with various buffer components such as Tris-HCl, KCl, and dNTPs to create a suitable reaction environment for DNA amplification and other molecular biology techniques. It is essential to optimize the concentration of magnesium chloride along with other reagents to achieve optimal results in specific experimental conditions.
How should Magnesium Chloride 100 mmol/L (100 mM) be stored and handled to maintain its stability and effectiveness?
Magnesium Chloride 100 mmol/L (100 mM) should be stored at room temperature in a dry and cool environment to prevent moisture absorption and degradation. It is crucial to handle the solution with proper pipetting techniques to avoid contamination and ensure accurate dispensing of the desired magnesium chloride concentration in molecular biology experiments.
