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Product Name | Phenol equilibrated, stabilized : Chloroform : Isoamyl Alcohol 25 : 24 : 1 |
Composition | Phenol, chloroform, isoamyl alcohol |
Ratio | 25 : 24 : 1 |
Application | commonly used in molecular biology for DNA extraction and purification |
Stability | stabilized for long-term storage |
Appearance | clear liquid |
Purity | high purity |
Storage Conditions | store at room temperature |
Manufacturing Method | specifically formulated for DNA extraction |
Packaging | available in various sizes |
Usage Instructions | handle with care and use in a well-ventilated area |
FAQ
What is the composition of Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 :
24 : 1?
Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1 contains a mixture of chloroform, isoamyl alcohol, and phenol. The ratio of the components is 25 parts chloroform, 24 parts isoamyl alcohol, and 1 part phenol.
What are the main applications of Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1?
Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1 is commonly used for the extraction and purification of nucleic acids, particularly DNA and RNA. It is also used in various molecular biology techniques such as DNA extraction, phenol-chloroform extraction, and RNA extraction from biological samples.
How is Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1 different from regular chloroform for nucleic acid extraction?
Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1 contains phenol which helps in denaturing proteins and removing contaminants during nucleic acid extraction. This makes it more efficient in separating nucleic acids from proteins, lipids, and other impurities compared to regular chloroform.
What precautions should be taken when using Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1?
When using Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1, it is important to work in a well-ventilated area to avoid inhaling harmful vapors. Additionally, proper personal protective equipment such as gloves, lab coats, and safety goggles should be worn to prevent skin contact and eye irritation.
Can Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1 be stored for long periods of time?
Yes, Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1 can be stored for long periods of time if kept in a cool, dry place away from direct sunlight. It is essential to store the reagent at the recommended temperature and conditions to ensure its stability and effectiveness.
Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1 contains a mixture of chloroform, isoamyl alcohol, and phenol. The ratio of the components is 25 parts chloroform, 24 parts isoamyl alcohol, and 1 part phenol.
What are the main applications of Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1?
Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1 is commonly used for the extraction and purification of nucleic acids, particularly DNA and RNA. It is also used in various molecular biology techniques such as DNA extraction, phenol-chloroform extraction, and RNA extraction from biological samples.
How is Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1 different from regular chloroform for nucleic acid extraction?
Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1 contains phenol which helps in denaturing proteins and removing contaminants during nucleic acid extraction. This makes it more efficient in separating nucleic acids from proteins, lipids, and other impurities compared to regular chloroform.
What precautions should be taken when using Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1?
When using Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1, it is important to work in a well-ventilated area to avoid inhaling harmful vapors. Additionally, proper personal protective equipment such as gloves, lab coats, and safety goggles should be worn to prevent skin contact and eye irritation.
Can Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1 be stored for long periods of time?
Yes, Phenol equilibrated, stabilized Chloroform : Isoamyl Alcohol 25 : 24 : 1 can be stored for long periods of time if kept in a cool, dry place away from direct sunlight. It is essential to store the reagent at the recommended temperature and conditions to ensure its stability and effectiveness.