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| Name | Phenol stabilized Chloroform Isoamyl Alcohol |
| Ratio | 25:24:1 |
| Stabilization | Phenol |
| Components | Chloroform, Isoamyl Alcohol |
| Ratio Chloroform | 25 |
| Ratio Isoamyl Alcohol | 1 |
| Stabilization Percentage | 4% |
| Used For | DNA extraction |
| Density | 1.062-1.066 g/mL |
| Appearance | Clear liquid |
| Miscible With Water | N/A |
FAQ
What is the composition of the Phenol stabilized Chloroform Isoamyl Alcohol (25:24:1)
solution?
The composition of the solution is as follows:
- Phenol: 25%
- Chloroform: 24%
- Isoamyl Alcohol: 1%
What is the purpose of using a Phenol stabilized Chloroform Isoamyl Alcohol solution in molecular biology applications?
This solution is commonly used in molecular biology for the extraction of DNA and RNA from samples. The combination of Phenol, Chloroform, and Isoamyl Alcohol helps to separate nucleic acids from other components in the sample, allowing for purification of the genetic material.
How does the Phenol stabilized Chloroform Isoamyl Alcohol solution work in DNA and RNA extraction procedures?
The Phenol stabilized Chloroform Isoamyl Alcohol solution works based on the principle of phase separation. When added to a sample, the solution forms distinct phases with different components of the sample. DNA and RNA migrate into the aqueous phase, while proteins and other contaminants remain in the organic phase. This enables the selective extraction and purification of nucleic acids.
What are the advantages of using Phenol stabilized Chloroform Isoamyl Alcohol solution over other extraction methods?
One of the main advantages of using this solution is its ability to effectively remove contaminants such as proteins, lipids, and polysaccharides from nucleic acid samples. Additionally, the use of Phenol stabilized Chloroform Isoamyl Alcohol solution ensures high yields of pure DNA and RNA, making it a reliable choice for molecular biology applications.
How should Phenol stabilized Chloroform Isoamyl Alcohol solution be stored and handled to maintain its stability and effectiveness?
It is important to store the solution in a cool, dark place away from direct sunlight to prevent degradation of the components. Additionally, proper handling practices should be followed to avoid inhalation or skin contact with the solution. It is recommended to use the solution in a fume hood and wear appropriate personal protective equipment when working with it.
The composition of the solution is as follows:
- Phenol: 25%
- Chloroform: 24%
- Isoamyl Alcohol: 1%
What is the purpose of using a Phenol stabilized Chloroform Isoamyl Alcohol solution in molecular biology applications?
This solution is commonly used in molecular biology for the extraction of DNA and RNA from samples. The combination of Phenol, Chloroform, and Isoamyl Alcohol helps to separate nucleic acids from other components in the sample, allowing for purification of the genetic material.
How does the Phenol stabilized Chloroform Isoamyl Alcohol solution work in DNA and RNA extraction procedures?
The Phenol stabilized Chloroform Isoamyl Alcohol solution works based on the principle of phase separation. When added to a sample, the solution forms distinct phases with different components of the sample. DNA and RNA migrate into the aqueous phase, while proteins and other contaminants remain in the organic phase. This enables the selective extraction and purification of nucleic acids.
What are the advantages of using Phenol stabilized Chloroform Isoamyl Alcohol solution over other extraction methods?
One of the main advantages of using this solution is its ability to effectively remove contaminants such as proteins, lipids, and polysaccharides from nucleic acid samples. Additionally, the use of Phenol stabilized Chloroform Isoamyl Alcohol solution ensures high yields of pure DNA and RNA, making it a reliable choice for molecular biology applications.
How should Phenol stabilized Chloroform Isoamyl Alcohol solution be stored and handled to maintain its stability and effectiveness?
It is important to store the solution in a cool, dark place away from direct sunlight to prevent degradation of the components. Additionally, proper handling practices should be followed to avoid inhalation or skin contact with the solution. It is recommended to use the solution in a fume hood and wear appropriate personal protective equipment when working with it.
