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Product Name | Sodium Pyruvate for cell culture |
CAS Number | 113-24-6 |
Formula | C3H3NaO3 |
Molecular Weight | 110.047 g/mol |
Appearance | White crystalline powder |
Solubility | Soluble in water |
Storage | Store at room temperature |
Shelf Life | 2 years |
Usage | Used as a cell culture supplement |
Sterility | Non-sterile |
Purity | ≥ 98% |
PH Range | 7.0 - 9.0 |
Melting Point | 145-148°C |
Boiling Point | 165-170°C |
Density | 1.27 g/cm3 |
FAQ
What is Sodium Pyruvate and how is it used in cell culture?
Sodium Pyruvate is a cell culture supplement that provides a source of pyruvate, an important metabolic intermediate in the glycolytic pathway. It is commonly added to cell culture media to support cell growth and viability.
What are the benefits of using Sodium Pyruvate in cell culture?
Sodium Pyruvate can enhance the growth and proliferation of many different types of cells in culture. It can also help to minimize cell stress and improve overall cell health. Additionally, Sodium Pyruvate is known to have antioxidant properties, which can protect cells from oxidative damage.
How should Sodium Pyruvate be added to cell culture media?
Sodium Pyruvate is typically added to cell culture media at a concentration of 1-2 mM. It is important to dissolve Sodium Pyruvate in sterile water before adding it to the media. Some cell types may require higher or lower concentrations of Sodium Pyruvate, so it is recommended to optimize the concentration for your specific cells.
Are there any cell types that benefit more from the addition of Sodium Pyruvate to media?
Sodium Pyruvate is particularly beneficial for cells that have high energy demands or are sensitive to oxidative stress. For example, fast-growing cells or cells that are under stress conditions may benefit from the addition of Sodium Pyruvate to their media. Additionally, Sodium Pyruvate has been shown to improve the culture of stem cells and other types of difficult-to-culture cells.
Are there any potential downsides or limitations to using Sodium Pyruvate in cell culture?
While Sodium Pyruvate is generally well-tolerated by most cells, some cell types may not respond well to its addition. In some cases, Sodium Pyruvate can interfere with specific metabolic pathways or signaling pathways in certain cells. It is always important to perform a viability assay and monitor cell growth when adding Sodium Pyruvate to cell culture media to ensure compatibility with your specific cell type.
Sodium Pyruvate is a cell culture supplement that provides a source of pyruvate, an important metabolic intermediate in the glycolytic pathway. It is commonly added to cell culture media to support cell growth and viability.
What are the benefits of using Sodium Pyruvate in cell culture?
Sodium Pyruvate can enhance the growth and proliferation of many different types of cells in culture. It can also help to minimize cell stress and improve overall cell health. Additionally, Sodium Pyruvate is known to have antioxidant properties, which can protect cells from oxidative damage.
How should Sodium Pyruvate be added to cell culture media?
Sodium Pyruvate is typically added to cell culture media at a concentration of 1-2 mM. It is important to dissolve Sodium Pyruvate in sterile water before adding it to the media. Some cell types may require higher or lower concentrations of Sodium Pyruvate, so it is recommended to optimize the concentration for your specific cells.
Are there any cell types that benefit more from the addition of Sodium Pyruvate to media?
Sodium Pyruvate is particularly beneficial for cells that have high energy demands or are sensitive to oxidative stress. For example, fast-growing cells or cells that are under stress conditions may benefit from the addition of Sodium Pyruvate to their media. Additionally, Sodium Pyruvate has been shown to improve the culture of stem cells and other types of difficult-to-culture cells.
Are there any potential downsides or limitations to using Sodium Pyruvate in cell culture?
While Sodium Pyruvate is generally well-tolerated by most cells, some cell types may not respond well to its addition. In some cases, Sodium Pyruvate can interfere with specific metabolic pathways or signaling pathways in certain cells. It is always important to perform a viability assay and monitor cell growth when adding Sodium Pyruvate to cell culture media to ensure compatibility with your specific cell type.