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| Product Name | alpha-Amanitine BioChemica |
| Product Type | Biochemical |
| CAS Number | 23109-05-9 |
| Molecular Formula | C39H54N10O14S |
| Molecular Weight | 918.97 g/mol |
| Appearance | White to off-white powder |
| Solubility | Soluble in water and organic solvents |
| Storage | Store at -20°C in a dry and dark place |
| Usage | Inhibitor of RNA polymerase II |
| Applications | Research in molecular biology and biochemistry |
| Synonyms | Amatoxins, RNA polymerase inhibitor |
| Purity | Minimum 95% |
FAQ
What is alpha-Amanitine and how is it used in the field of biochemistry?
Alpha-Amanitine is a cyclic peptide found in the deadly Amanita mushroom species. It is a potent inhibitor of RNA polymerase II, which is an essential enzyme involved in transcription. In biochemistry, alpha-Amanitine is commonly used to study transcription processes and to elucidate the mechanisms underlying gene expression.
How does alpha-Amanitine inhibit RNA polymerase II activity?
Alpha-Amanitine exerts its inhibitory effect on RNA polymerase II by binding tightly to the enzyme's active site. This binding prevents the enzyme from catalyzing the formation of phosphodiester bonds essential for RNA synthesis, effectively halting the transcription process.
What are the applications of alpha-Amanitine in research and drug development?
Alpha-Amanitine is widely used in research to study transcriptional regulation, RNA processing, and gene expression. It is also utilized in drug development to screen potential inhibitors of RNA polymerase II as well as to investigate the effects of transcriptional dysregulation in diseases such as cancer.
What are the safety considerations when working with alpha-Amanitine in a laboratory setting?
Alpha-Amanitine is a highly toxic compound and should be handled with extreme caution. It is essential to use appropriate personal protective equipment, such as gloves, lab coats, and safety goggles, when working with alpha-Amanitine. Additionally, proper ventilation and containment measures should be in place to minimize exposure and ensure the safety of laboratory personnel.
What are the recommended storage conditions for alpha-Amanitine to maintain its stability and potency?
Alpha-Amanitine should be stored in a cool, dry place away from light and moisture. It is recommended to store the compound at a temperature of -20°C or below in a tightly sealed container to prevent degradation. Proper storage conditions are crucial to maintaining the stability and potency of alpha-Amanitine for long-term use in biochemistry research.
Alpha-Amanitine is a cyclic peptide found in the deadly Amanita mushroom species. It is a potent inhibitor of RNA polymerase II, which is an essential enzyme involved in transcription. In biochemistry, alpha-Amanitine is commonly used to study transcription processes and to elucidate the mechanisms underlying gene expression.
How does alpha-Amanitine inhibit RNA polymerase II activity?
Alpha-Amanitine exerts its inhibitory effect on RNA polymerase II by binding tightly to the enzyme's active site. This binding prevents the enzyme from catalyzing the formation of phosphodiester bonds essential for RNA synthesis, effectively halting the transcription process.
What are the applications of alpha-Amanitine in research and drug development?
Alpha-Amanitine is widely used in research to study transcriptional regulation, RNA processing, and gene expression. It is also utilized in drug development to screen potential inhibitors of RNA polymerase II as well as to investigate the effects of transcriptional dysregulation in diseases such as cancer.
What are the safety considerations when working with alpha-Amanitine in a laboratory setting?
Alpha-Amanitine is a highly toxic compound and should be handled with extreme caution. It is essential to use appropriate personal protective equipment, such as gloves, lab coats, and safety goggles, when working with alpha-Amanitine. Additionally, proper ventilation and containment measures should be in place to minimize exposure and ensure the safety of laboratory personnel.
What are the recommended storage conditions for alpha-Amanitine to maintain its stability and potency?
Alpha-Amanitine should be stored in a cool, dry place away from light and moisture. It is recommended to store the compound at a temperature of -20°C or below in a tightly sealed container to prevent degradation. Proper storage conditions are crucial to maintaining the stability and potency of alpha-Amanitine for long-term use in biochemistry research.
