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| Name | 2-Nitrophenyl-ß-D-Galactopyranoside BioChemica |
| Cas Number | 3630-20-8 |
| Molecular Formula | C12H15NO8 |
| Molecular Weight | 301.25 g/mol |
| Appearance | Yellow powder |
| Solubility | Soluble in water |
| Storage Temperature | Room temperature |
| Purity | ≥98% |
| Usage | Substrate for detecting beta-galactosidase activity |
| Applications | Enzyme assays, substrate specificity studies |
| Format | Solid |
| Melting Point | ≥100°C |
| Boiling Point | Not available |
| Flash Point | Not available |
| Specific Gravity | Not available |
| Product Code | B1526 |
FAQ
What is 2-Nitrophenyl-β-D-Galactopyranoside BioChemica and how is it used in scientific
research?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica is a substrate commonly used in enzymatic assays to detect the presence of β-galactosidase. This compound is cleaved by the enzyme β-galactosidase, resulting in the release of the yellow-colored product 2-nitrophenol. This reaction is often used to quantitatively measure the activity of β-galactosidase in various biological samples.
How is 2-Nitrophenyl-β-D-Galactopyranoside BioChemica different from other substrates used in β-galactosidase assays?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica offers several advantages over other substrates commonly used in β-galactosidase assays. Firstly, it provides a clear and distinct yellow color upon cleavage by the enzyme, making it easy to detect and measure the enzyme activity. Secondly, this substrate is highly specific to β-galactosidase, resulting in minimal background interference from other enzymes present in the sample. Lastly, 2-Nitrophenyl-β-D-Galactopyranoside BioChemica is stable and can be stored for extended periods without significant degradation, ensuring reliable and reproducible results.
What are the applications of using 2-Nitrophenyl-β-D-Galactopyranoside BioChemica in scientific research?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica is commonly used in a wide range of applications in scientific research. Some of the key applications include measuring β-galactosidase activity in bacterial cultures, studying gene expression and promoter activity in molecular biology, detecting lactose metabolism in dairy microbiology, and assessing the functionality of transgenic cells and organisms. Additionally, this substrate is also utilized in high-throughput screening assays to identify potential inhibitors or activators of β-galactosidase for drug discovery and development.
How should 2-Nitrophenyl-β-D-Galactopyranoside BioChemica be stored and handled in the laboratory?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica should be stored in a cool, dry place away from direct sunlight and moisture. It is recommended to store the substrate at -20°C or below to maintain its stability and integrity. When handling the compound in the laboratory, it is important to wear appropriate personal protective equipment, such as gloves and safety glasses, to prevent skin contact and eye exposure. Additionally, ensure that the substrate is properly sealed and stored in a secure location to avoid contamination and accidental spills.
What are the key features and benefits of using 2-Nitrophenyl-β-D-Galactopyranoside BioChemica in enzyme assays?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica offers several key features and benefits that make it an ideal substrate for enzyme assays, particularly those involving β-galactosidase. Some of the key features include its high specificity for β-galactosidase, resulting in accurate and reliable measurements of enzyme activity. The clear and distinct yellow color produced upon cleavage by the enzyme enables easy detection and quantification of the reaction product. Additionally, the stability and long shelf life of the substrate ensure consistent and reproducible results, making it a valuable tool for research applications.
2-Nitrophenyl-β-D-Galactopyranoside BioChemica is a substrate commonly used in enzymatic assays to detect the presence of β-galactosidase. This compound is cleaved by the enzyme β-galactosidase, resulting in the release of the yellow-colored product 2-nitrophenol. This reaction is often used to quantitatively measure the activity of β-galactosidase in various biological samples.
How is 2-Nitrophenyl-β-D-Galactopyranoside BioChemica different from other substrates used in β-galactosidase assays?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica offers several advantages over other substrates commonly used in β-galactosidase assays. Firstly, it provides a clear and distinct yellow color upon cleavage by the enzyme, making it easy to detect and measure the enzyme activity. Secondly, this substrate is highly specific to β-galactosidase, resulting in minimal background interference from other enzymes present in the sample. Lastly, 2-Nitrophenyl-β-D-Galactopyranoside BioChemica is stable and can be stored for extended periods without significant degradation, ensuring reliable and reproducible results.
What are the applications of using 2-Nitrophenyl-β-D-Galactopyranoside BioChemica in scientific research?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica is commonly used in a wide range of applications in scientific research. Some of the key applications include measuring β-galactosidase activity in bacterial cultures, studying gene expression and promoter activity in molecular biology, detecting lactose metabolism in dairy microbiology, and assessing the functionality of transgenic cells and organisms. Additionally, this substrate is also utilized in high-throughput screening assays to identify potential inhibitors or activators of β-galactosidase for drug discovery and development.
How should 2-Nitrophenyl-β-D-Galactopyranoside BioChemica be stored and handled in the laboratory?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica should be stored in a cool, dry place away from direct sunlight and moisture. It is recommended to store the substrate at -20°C or below to maintain its stability and integrity. When handling the compound in the laboratory, it is important to wear appropriate personal protective equipment, such as gloves and safety glasses, to prevent skin contact and eye exposure. Additionally, ensure that the substrate is properly sealed and stored in a secure location to avoid contamination and accidental spills.
What are the key features and benefits of using 2-Nitrophenyl-β-D-Galactopyranoside BioChemica in enzyme assays?
2-Nitrophenyl-β-D-Galactopyranoside BioChemica offers several key features and benefits that make it an ideal substrate for enzyme assays, particularly those involving β-galactosidase. Some of the key features include its high specificity for β-galactosidase, resulting in accurate and reliable measurements of enzyme activity. The clear and distinct yellow color produced upon cleavage by the enzyme enables easy detection and quantification of the reaction product. Additionally, the stability and long shelf life of the substrate ensure consistent and reproducible results, making it a valuable tool for research applications.
