D(-)-Sorbitol for molecular biology

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Name D(-)-Sorbitol
Product Type molecular biology reagent
Synonyms N/A
Molecular Formula C6H14O6
Molecular Weight 182.17 g/mol
Purity ≥ 98%
Solubility Soluble in water
Appearance White crystalline powder
Storage Conditions Store at room temperature
Shelf Life 2 years
Applications Used as a cryoprotectant and stabilizer in molecular biology experiments
FAQ
What is D(-)Sorbitol and how is it used in molecular biology?
D(-)Sorbitol is a sugar alcohol that is commonly used as a carbon source in bacterial growth media for molecular biology applications. It is also used in various biochemical procedures as a cryoprotectant and stabilizer for enzymes and proteins.

What are the benefits of using D(-)Sorbitol in molecular biology experiments?
D(-)Sorbitol is non-toxic to most organisms, making it a safe and reliable carbon source for bacterial growth. It can also help maintain the stability and activity of enzymes and proteins during various experimental procedures. Additionally, D(-)Sorbitol has a low osmotic pressure, making it suitable for use in cell culture applications.

How can D(-)Sorbitol be incorporated into experimental protocols?
D(-)Sorbitol can be added directly to growth media to support the growth of bacteria or other microorganisms. It can also be used as a cryoprotectant to preserve the viability of cells during freezing and thawing processes. Furthermore, D(-)Sorbitol can be included in enzyme or protein solutions to enhance their stability and activity.

Are there any specific considerations to keep in mind when using D(-)Sorbitol in molecular biology experiments?
It is important to ensure that D(-)Sorbitol is properly dissolved in the experimental solution to avoid any precipitation or uneven distribution. Additionally, the concentration of D(-)Sorbitol should be optimized based on the specific experimental requirements to achieve the desired results. Finally, researchers should be aware of any potential interactions between D(-)Sorbitol and other components in the experimental system.

In summary, D(-)Sorbitol is a versatile and effective reagent for molecular biology experiments, offering benefits such as non-toxicity, stability enhancement, and cryoprotection. By carefully considering its incorporation into experimental protocols and addressing any specific considerations, researchers can leverage the unique properties of D(-)Sorbitol to achieve successful outcomes in their studies.
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